##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926853_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 682476 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.055341140201268 31.0 30.0 33.0 26.0 34.0 2 30.350925160738253 31.0 30.0 34.0 26.0 34.0 3 30.415684947162976 31.0 30.0 34.0 26.0 34.0 4 34.00991390173427 37.0 35.0 37.0 30.0 37.0 5 34.01301437706234 37.0 35.0 37.0 30.0 37.0 6 33.977221177008424 37.0 35.0 37.0 30.0 37.0 7 33.97571489693411 37.0 35.0 37.0 30.0 37.0 8 34.00513571173199 37.0 35.0 37.0 30.0 37.0 9 35.57429858339341 39.0 35.0 39.0 30.0 39.0 10 35.460332671038984 39.0 35.0 39.0 30.0 39.0 11 35.507843499258584 39.0 35.0 39.0 30.0 39.0 12 35.40835282119811 39.0 35.0 39.0 30.0 39.0 13 35.438651322537346 39.0 35.0 39.0 30.0 39.0 14 36.54243079610126 40.0 37.0 41.0 30.0 41.0 15 36.54348870876046 40.0 37.0 41.0 30.0 41.0 16 36.531310991155735 40.0 37.0 41.0 30.0 41.0 17 36.50448953516314 40.0 37.0 41.0 30.0 41.0 18 36.481262344756445 40.0 37.0 41.0 30.0 41.0 19 36.53541516478235 40.0 37.0 41.0 30.0 41.0 20 36.50092603989005 40.0 37.0 41.0 30.0 41.0 21 36.4524730539975 40.0 37.0 41.0 30.0 41.0 22 36.36730082816099 40.0 37.0 41.0 30.0 41.0 23 36.29763244421782 40.0 37.0 41.0 30.0 41.0 24 36.23001834496744 40.0 37.0 41.0 30.0 41.0 25 36.120710471870076 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.13668386603790594 1101 2 -0.13373966798043213 1101 3 0.1568451484129305 1101 4 0.020065215575513662 1101 5 0.0021732838966030954 1101 6 -0.04262437775237515 1101 7 0.20667626207102785 1101 8 -0.002961179021959026 1101 9 -0.1629806911531162 1101 10 0.031708418008562944 1101 11 0.0018221239455016303 1101 12 -0.10909828970745394 1101 13 0.17024292408126485 1101 14 -0.059937504152578924 1101 15 -0.019603087898893534 1101 16 0.057075818754235286 1101 17 0.2555009003240798 1101 18 0.0603996198390746 1101 19 0.0958051670779625 1101 20 0.1329236520618906 1101 21 0.09914828200557224 1101 22 0.03260645203447865 1101 23 -0.18269039800092912 1101 24 0.17246647765226442 1101 25 -0.07003169401124865 1102 1 -0.5858552501930134 1102 2 -0.5803161890649164 1102 3 -0.3682675335647936 1102 4 -0.5570376110066775 1102 5 -0.7486377330276923 1102 6 -0.5883463734699674 1102 7 -0.6177813489732742 1102 8 -0.6114819897455703 1102 9 -0.5660898337923683 1102 10 -0.5248525573627276 1102 11 -0.4170918271099424 1102 12 -0.43983284909892006 1102 13 -0.59191353032287 1102 14 -0.5315680441461268 1102 15 -0.6260462482739442 1102 16 -0.6056897538462067 1102 17 -0.7298108449999816 1102 18 -0.58980521204235 1102 19 -0.6586986900187952 1102 20 -0.5882449162659213 1102 21 -0.7278944149312352 1102 22 -0.7304739457011209 1102 23 -0.7246726230911236 1102 24 -0.7866160319538125 1102 25 -0.779710122623186 1103 1 -0.8916804929114583 1103 2 -0.8880831793561796 1103 3 -0.7119568539531436 1103 4 -0.9482997469290098 1103 5 -0.8583464708917674 1103 6 -0.9019386064796748 1103 7 -0.8566163004295788 1103 8 -0.8833266499397396 1103 9 -0.9359927464137243 1103 10 -0.632619547653988 1103 11 -0.8471889144885836 1103 12 -1.0029396452154273 1103 13 -0.6482242099345186 1103 14 -0.8878787237577797 1103 15 -0.9241045007011337 1103 16 -0.9416120839432907 1103 17 -0.8861215246116387 1103 18 -0.9111644353433164 1103 19 -1.0140384958440407 1103 20 -0.9892157900523273 1103 21 -1.019156550853566 1103 22 -0.9819302563807355 1103 23 -1.2062260211493765 1103 24 -1.1429267115654653 1103 25 -1.282622743982408 1104 1 -0.4319295023197327 1104 2 -0.3734261776097334 1104 3 -0.1807968757367746 1104 4 -0.3789376692215498 1104 5 -0.5552460596318909 1104 6 -0.4331267531811491 1104 7 -0.4944540875133896 1104 8 -0.4408260396225643 1104 9 -0.5603171722461013 1104 10 -0.5536219701437091 1104 11 -0.4174035583450362 1104 12 -0.5412149827677766 1104 13 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1105 24 -0.305483780681989 1105 25 -0.31406526489998754 1106 1 0.3912683593062667 1106 2 0.3601783998941066 1106 3 0.5365321116747168 1106 4 0.4997759604690657 1106 5 0.49165211384031693 1106 6 0.5898613312485352 1106 7 0.37869085988253204 1106 8 0.46663796836250526 1106 9 0.22692062975504967 1106 10 0.3497216828742822 1106 11 0.20689318992044292 1106 12 0.17917325542332918 1106 13 0.10277355394218546 1106 14 0.18566803197641946 1106 15 0.19020668840696686 1106 16 0.14447771952317368 1106 17 -0.03713061063162115 1106 18 0.10687362592834404 1106 19 0.11377057507974797 1106 20 0.14440342739887058 1106 21 0.15323534626588753 1106 22 0.13698432748332579 1106 23 0.16312682401087386 1106 24 0.42756117845878094 1106 25 0.30125829803218096 1107 1 0.5240173052689627 1107 2 0.3304467196692471 1107 3 0.2497135302315172 1107 4 0.4288898094677407 1107 5 0.2867228045161525 1107 6 0.45727914135228787 1107 7 0.29448923697543705 1107 8 0.26142239445760396 1107 9 0.20402687467709768 1107 10 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22 0.7509554917711654 1108 23 0.7063971477175102 1108 24 0.5967026734271599 1108 25 0.6148754237534604 1109 1 0.5500143051340558 1109 2 0.28149216572712277 1109 3 0.34266321319820037 1109 4 0.4550869374505666 1109 5 0.4485925014560692 1109 6 0.6123078516963147 1109 7 0.32159408892083263 1109 8 0.31142226044323706 1109 9 0.4570710109679226 1109 10 0.3770546136366306 1109 11 0.33737719500996377 1109 12 0.3442164316173546 1109 13 0.2395566109861278 1109 14 0.39033530523195026 1109 15 0.366270473138961 1109 16 0.2011272905846937 1109 17 0.3410283648343224 1109 18 0.32335936334823145 1109 19 0.39638816087428097 1109 20 0.18671254544962324 1109 21 0.330912147338438 1109 22 0.32412883316349195 1109 23 0.23485094812296836 1109 24 0.19335685273330938 1109 25 0.24680282994322056 1110 1 0.2854217109901178 1110 2 0.1423718838319772 1110 3 0.27632395818187305 1110 4 0.336545943801589 1110 5 0.3236498770317908 1110 6 0.28545992184199775 1110 7 0.3605466508820925 1110 8 0.28334182468555724 1110 9 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23 0.1728887972106179 2209 24 0.23817999338918128 2209 25 0.08970051596563167 2210 1 -0.19804201067782046 2210 2 -0.115698644952694 2210 3 -0.046583485947891745 2210 4 -0.051566885685680575 2210 5 -0.12088459950780361 2210 6 -0.1262617391897578 2210 7 -0.3018640577021401 2210 8 -0.1192543787789262 2210 9 -0.14475580462116255 2210 10 -0.1762962709774527 2210 11 -0.3512424700101491 2210 12 -0.14410235430420926 2210 13 -0.021772173259066108 2210 14 -0.032735972018933523 2210 15 -0.12029028941235964 2210 16 -0.06555460533580515 2210 17 -0.10759592107726235 2210 18 -0.28882624014280367 2210 19 -0.21261232814111963 2210 20 0.047656718653144026 2210 21 -0.042485415228171064 2210 22 -0.1986051711255996 2210 23 -0.24991444174737865 2210 24 -0.043362210094308296 2210 25 0.08450791457414653 2211 1 0.15707557504970637 2211 2 0.2527738309236618 2211 3 0.34749310321418747 2211 4 0.2547752816261024 2211 5 0.3065289574348995 2211 6 0.29511664766081225 2211 7 0.26047750311125384 2211 8 0.2916840582270197 2211 9 0.2818417053281408 2211 10 0.37373217993025065 2211 11 0.34423741332409463 2211 12 0.3584003033853733 2211 13 0.3241576432922102 2211 14 0.23306109634501837 2211 15 0.2603436717717429 2211 16 0.2660977961252442 2211 17 0.47741181348088446 2211 18 0.20138375140710707 2211 19 0.3481848765146083 2211 20 0.15355798207788496 2211 21 0.1116401367535147 2211 22 0.2542276533851151 2211 23 0.1892551324771219 2211 24 0.1954388652477803 2211 25 0.05268208183141354 2212 1 -0.05607677226114305 2212 2 -0.19336855606759684 2212 3 -0.09716629541308563 2212 4 -0.02276054469166411 2212 5 -0.12174886577277988 2212 6 -0.24757498459828753 2212 7 -0.06061042385534421 2212 8 -0.22663629407069408 2212 9 -0.3231731413124308 2212 10 -0.17186426625989526 2212 11 -0.19776550908461843 2212 12 -0.1664854143474841 2212 13 -0.31918030378270146 2212 14 -0.36876658208277746 2212 15 -0.3980847292149079 2212 16 -0.2563698965124317 2212 17 -0.3172797584532674 2212 18 -0.36658654860897855 2212 19 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0.14073725630805 2214 6 0.10886044482818846 2214 7 -0.054468092877584695 2214 8 0.07162241757779242 2214 9 -0.17579004025234468 2214 10 -0.2578793289087855 2214 11 -0.08730528284048944 2214 12 -0.0684676538150839 2214 13 0.035533702942785794 2214 14 -0.24850970640490289 2214 15 -0.18652195936648042 2214 16 -0.07371405128507291 2214 17 -0.19109362692023524 2214 18 -0.26055443995027616 2214 19 -0.14029303881672206 2214 20 -0.3155522345043593 2214 21 -0.23027808923635718 2214 22 -0.0699931157661311 2214 23 -0.1666130056392845 2214 24 -0.30677573983854245 2214 25 -0.1761798276072284 2215 1 0.388138609237739 2215 2 0.23622185757021796 2215 3 0.4099915320729117 2215 4 0.3433358019880899 2215 5 0.3067850534630523 2215 6 0.16925880452355813 2215 7 0.0695806956703251 2215 8 0.17487598884542166 2215 9 0.0466559964291946 2215 10 0.18269868319822535 2215 11 -0.046392300099739714 2215 12 -0.10395200933459137 2215 13 0.10242718828969544 2215 14 -0.06407157082441017 2215 15 0.03771609510004481 2215 16 0.0822365593228298 2215 17 0.170604058327541 2215 18 0.06527735371982857 2215 19 -0.21333316018724702 2215 20 -0.0637324276757667 2215 21 0.11778644142923156 2215 22 0.0788945714343825 2215 23 0.15533733458873655 2215 24 0.1053763338249567 2215 25 0.09352861275147717 2216 1 -0.21873664961989547 2216 2 -0.04792664199678498 2216 3 -1.9692170224660401 2216 4 -1.498378871004114 2216 5 -0.4201060192429935 2216 6 -0.17200404422827376 2216 7 -0.2669794157796517 2216 8 -0.2489012618040789 2216 9 -0.184429511308295 2216 10 -0.2599836467087755 2216 11 -0.14877567296769456 2216 12 -0.1063655038185658 2216 13 -0.5156721377391449 2216 14 -0.6282833257850626 2216 15 -0.44811544976233364 2216 16 -0.46370267031684165 2216 17 -0.275745578330465 2216 18 -0.44061142221300287 2216 19 -0.46085000946621335 2216 20 -0.44109979843520364 2216 21 -0.369890052414668 2216 22 -0.5617771406029703 2216 23 -0.6720658055897388 2216 24 -0.8040404363847671 2216 25 -0.5878449329392552 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14595.0 3 22.0 4 228.0 5 1493.0 6 2029.0 7 1565.0 8 1177.0 9 1012.0 10 913.0 11 826.0 12 773.0 13 768.0 14 772.0 15 770.0 16 827.0 17 778.0 18 951.0 19 1098.0 20 1490.0 21 1952.0 22 2403.0 23 2923.0 24 3677.0 25 4277.0 26 5222.0 27 5639.0 28 6667.0 29 8233.0 30 10461.0 31 13539.0 32 17599.0 33 23367.0 34 32011.0 35 45212.0 36 66952.0 37 116451.0 38 283804.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.601995158805835 19.61725225555637 10.746130712242353 28.034621873395437 2 13.775350052951651 20.850482725440557 41.11042986179766 24.263737359810136 3 18.710761871823582 24.88217570474756 29.34586999466567 27.0611924287632 4 11.93272730469643 17.57556309672428 38.38772352434371 32.10398607423558 5 12.608415965629263 38.918529220841066 35.658760244475346 12.814294569054322 6 31.491392644784128 38.205835475124836 17.862099994706785 12.440671885384258 7 27.83974418158184 31.816536456615946 21.81666835199653 18.52705100980569 8 24.523131615234583 39.091997461977286 19.8272034418636 16.557667480924533 9 26.091671221555146 15.776863706502036 20.03423126915068 38.09723380279213 10 14.562571891186964 30.086965440944823 34.30966144287902 21.040801224989195 11 34.21256617691233 22.683679853938234 24.75342552066009 18.350328448489343 12 23.190514496544186 25.04172326231221 32.11335833065924 19.654403910484373 13 29.18508012050311 22.38817123632911 25.71123797020244 22.71551067296534 14 20.207890094303682 23.183672392875355 27.46646035904559 29.141977153775372 15 22.364225679018134 32.348459094798784 23.162811961503746 22.124503264679333 16 21.22584142892728 28.87819244801969 28.84537049247586 21.050595630577167 17 20.979993377037072 29.54368957645273 28.34965141529201 21.126665631218184 18 20.7837098078749 28.353837227021767 30.529164566458405 20.333288398644925 19 23.199091541814717 27.370233341880656 27.438514231290522 21.992160885014105 20 22.99446278460833 28.261484337109305 28.46002699007873 20.284025888203637 21 22.238228104831855 27.152734221586545 27.382808607419822 23.226229066161775 22 22.42100821127776 29.164688575129382 27.388215849348548 21.02608736424431 23 21.67548748966997 28.65009172483721 28.01607675581266 21.658344029680165 24 22.850980403649075 27.40206952938867 28.714353953410683 21.03259611355158 25 22.205208084532096 28.041773696989768 28.308464199730672 21.444554018747464 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 184.0 1 184.0 2 192.5 3 201.0 4 201.0 5 201.0 6 353.5 7 506.0 8 506.0 9 506.0 10 838.0 11 1170.0 12 1170.0 13 1170.0 14 1988.0 15 2806.0 16 2806.0 17 2806.0 18 5152.5 19 7499.0 20 7499.0 21 7499.0 22 12102.0 23 16705.0 24 16705.0 25 16705.0 26 25082.5 27 33460.0 28 33460.0 29 33460.0 30 44814.0 31 56168.0 32 56168.0 33 56168.0 34 63292.5 35 70417.0 36 70417.0 37 70417.0 38 75582.0 39 80747.0 40 80747.0 41 80747.0 42 84314.5 43 87882.0 44 87882.0 45 87882.0 46 89866.5 47 91851.0 48 91851.0 49 91851.0 50 86958.5 51 82066.0 52 82066.0 53 82066.0 54 71172.0 55 60278.0 56 60278.0 57 60278.0 58 51074.5 59 41871.0 60 41871.0 61 41871.0 62 33717.5 63 25564.0 64 25564.0 65 25564.0 66 19571.5 67 13579.0 68 13579.0 69 13579.0 70 9755.0 71 5931.0 72 5931.0 73 5931.0 74 4136.0 75 2341.0 76 2341.0 77 2341.0 78 1590.0 79 839.0 80 839.0 81 839.0 82 571.5 83 304.0 84 304.0 85 304.0 86 198.5 87 93.0 88 93.0 89 93.0 90 52.5 91 12.0 92 12.0 93 12.0 94 7.0 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12102989702201983 2 0.10579126591997373 3 0.015238631102046079 4 0.0 5 0.005128385467034738 6 0.3457997057771995 7 0.01714345998980184 8 0.008058891448197446 9 0.008644992644429987 10 0.003077031280220843 11 0.0036631324764533845 12 4.3957589717440616E-4 13 5.861011962325415E-4 14 0.0 15 0.0029305059811627074 16 8.791517943488123E-4 17 2.9305059811627075E-4 18 5.861011962325415E-4 19 1.4652529905813538E-4 20 4.3957589717440616E-4 21 0.012894226317115913 22 0.0 23 0.0 24 2.9305059811627075E-4 25 0.005421436065151009 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 682476.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.37705012094384 #Duplication Level Percentage of deduplicated Percentage of total 1 91.58716452249129 74.53093277781902 2 6.368102462084792 10.364347864647597 3 1.0281109284044718 2.5099390365198238 4 0.35635627347498394 1.1599688930994616 5 0.16960580592448904 0.6901010084760107 6 0.10001743585094894 0.48834743341265735 7 0.06768200580532437 0.38554333850941247 8 0.049061981606004286 0.3194015468947708 9 0.040801783995860916 0.29882959391295877 >10 0.1941247096638885 2.9563095515043285 >50 0.019125389054168693 1.098262339626892 >100 0.017140333240906157 2.9902213490987615 >500 0.0016238210417078081 0.9618452344683002 >1k 0.0010825473611385387 1.2459500320100743 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1769 0.2592032540338415 No Hit CTGTAGGACGTGGAATATGGCAAGA 1563 0.2290190424278656 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1317 0.19297381885956427 No Hit CTTTAGGACGTGAAATATGGCGAGG 1298 0.1901898381774597 No Hit GTATCAACGCAGAGTACTTTTTTTT 1298 0.1901898381774597 No Hit GTCCTACAGTGTGCATTTCTCATTT 1241 0.18183789613114598 No Hit CTGTAGGACCTGGAATATGGCGAGA 957 0.14022471119863555 No Hit GGTATCAACGCAGAGTACTTTTTTT 822 0.12044379582578728 No Hit GTACATGGGAAGCAGTGGTATCAAC 808 0.11839244163897339 No Hit GATATACACTGTTCTACAAATCCCG 756 0.11077312608795034 No Hit CTGAAGGACCTGGAATATGGCGAGA 715 0.10476558882656681 No Hit CCCATGTACTCTGCGTTGATACCAC 690 0.10110245635011342 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 1.4652529905813538E-4 4 0.0 0.0 0.0 0.0 1.4652529905813538E-4 5 0.0 0.0 0.0 0.0 1.4652529905813538E-4 6 1.4652529905813538E-4 0.0 0.0 0.0 1.4652529905813538E-4 7 1.4652529905813538E-4 0.0 0.0 0.0 1.4652529905813538E-4 8 1.4652529905813538E-4 0.0 0.0 0.0 1.4652529905813538E-4 9 1.4652529905813538E-4 0.0 0.0 1.4652529905813538E-4 1.4652529905813538E-4 10 1.4652529905813538E-4 0.0 0.0 1.4652529905813538E-4 1.4652529905813538E-4 11 1.4652529905813538E-4 0.0 0.0 1.4652529905813538E-4 1.4652529905813538E-4 12 1.4652529905813538E-4 0.0 0.0 1.4652529905813538E-4 1.4652529905813538E-4 13 1.4652529905813538E-4 0.0 0.0 1.4652529905813538E-4 1.4652529905813538E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCCTA 25 0.006080098 18.972086 11 CCAACGA 50 4.6060886E-6 17.079884 19 GTTTAAG 50 8.434114E-5 15.25591 1 GTATTAC 40 0.0051481095 14.302416 1 TGTCGAG 40 0.0053164423 14.233235 18 AGACTGT 110 1.7826096E-10 13.852687 6 TCCAACG 85 2.7303577E-7 13.3959875 18 GGACGAG 50 0.0014701434 13.33321 6 TAGGACT 115 3.601599E-10 13.250396 4 TAGGACC 390 0.0 12.942494 4 GTGTAGA 210 0.0 12.713258 1 TGTAGAA 405 0.0 12.712322 2 CTTATAG 75 1.4361551E-5 12.700164 3 GGCGAGG 365 0.0 12.478454 19 TCCTAGG 100 1.3719364E-7 12.394514 2 AATGCCG 115 5.3951226E-9 12.376727 18 ATGCCGG 115 5.3951226E-9 12.376727 19 AATCCCG 185 0.0 12.309825 19 GTCTTAC 70 1.05245665E-4 12.259213 1 CTGTACT 135 3.5470293E-10 11.992835 4 >>END_MODULE