FastQCFastQC Report
Thu 26 May 2016
SRR1926852_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926852_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641962
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15680.2442512173617753No Hit
GTCCTACAGTGTGCATTTCTCATTT13750.21418713257170982No Hit
CTGTAGGACGTGGAATATGGCAAGA12910.20110224592732903No Hit
GTCCTAAAGTGTGTATTTCTCATTT12440.19378094030487786No Hit
CTTTAGGACGTGAAATATGGCGAGG10230.15935522663335214No Hit
CTGTAGGACCTGGAATATGGCGAGA9210.14346643570803255No Hit
GTATCAACGCAGAGTACTTTTTTTT6820.10623681775556808No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAAC500.001458990313.3464851
CCAACGA655.5285087E-513.131947519
TGGACTG801.9549734E-613.085615
ATAAGAC905.1896313E-712.7059633
TCTAGGA1301.8735591E-1012.46161652
TCCAACG1001.4688158E-712.3294418
GATATAC2500.012.2024991
GTCTAGG550.002985983412.1331681
TAGGACC3250.012.0114534
CTTATAC802.7709253E-511.911843
TAAGACA1459.276846E-1111.8195114
ACTGTTC2550.011.5604148
GTCCTAC8650.011.4618851
GGACGTG6900.011.4468596
CTAGGGC752.0114594E-411.4353673
AGGACGT7250.011.4201695
TAGGACG7250.011.2941994
TTAGGAC4650.011.2714183
CCCTTTA600.005718766211.1220691
GTTTTAG600.005718766211.1220691
Produced by FastQC (version 0.11.5)