##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926852_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 641962 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.893568466669368 31.0 28.0 31.0 16.0 34.0 2 28.028478321146736 31.0 28.0 31.0 16.0 34.0 3 27.520764468924952 31.0 27.0 31.0 10.0 34.0 4 31.075942189724625 35.0 32.0 37.0 16.0 37.0 5 31.44188441060374 35.0 32.0 37.0 17.0 37.0 6 30.670475822556476 35.0 31.0 37.0 11.0 37.0 7 30.741782223869947 35.0 32.0 37.0 11.0 37.0 8 30.64854150245653 35.0 31.0 37.0 10.0 37.0 9 32.65430975665226 37.0 32.0 39.0 16.0 39.0 10 32.79399559475483 37.0 33.0 39.0 15.0 39.0 11 32.98208928254321 37.0 33.0 39.0 16.0 39.0 12 32.84755172424536 37.0 33.0 39.0 11.0 39.0 13 32.96480321265122 38.0 33.0 39.0 12.0 39.0 14 33.94428174876395 39.0 33.0 40.0 11.0 41.0 15 33.53787295821248 39.0 33.0 40.0 10.0 41.0 16 33.61318426947389 39.0 33.0 40.0 10.0 41.0 17 33.830658200952705 39.0 33.0 40.0 10.0 41.0 18 33.862393724239126 39.0 33.0 41.0 10.0 41.0 19 33.22021085360193 39.0 32.0 40.0 8.0 41.0 20 33.513602051211755 39.0 33.0 40.0 2.0 41.0 21 33.50543645885582 39.0 33.0 40.0 2.0 41.0 22 33.528179238023434 39.0 33.0 41.0 2.0 41.0 23 33.503264990762695 39.0 34.0 41.0 2.0 41.0 24 32.18329433829417 38.0 31.0 40.0 2.0 41.0 25 32.765668372894346 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.7539853345551855 1101 2 -0.8640611219043919 1101 3 -1.5476028317916715 1101 4 -1.1925683745704347 1101 5 -1.120476168744407 1101 6 -2.728670002277102 1101 7 -1.194191910015384 1101 8 -0.6194807389197763 1101 9 -0.7616687180788126 1101 10 -9.11749823697794 1101 11 -4.5696428169247625 1101 12 -2.2312818420158678 1101 13 -1.5061782713886664 1101 14 -1.2831930208018463 1101 15 -2.188124301554897 1101 16 -1.1049135951194842 1101 17 -0.8847494776470626 1101 18 -0.7861329351758783 1101 19 -1.5914166880886604 1101 20 -0.9257946510556607 1101 21 -0.9689224595860821 1101 22 -0.6868467134157683 1101 23 -0.7604972624042574 1101 24 -0.926303123565134 1101 25 -0.5870524662472434 1102 1 -0.4716434944917758 1102 2 -0.4818459931172079 1102 3 -1.1961499656904415 1102 4 -1.1318289199141987 1102 5 -0.8387047990224694 1102 6 -1.668834241658967 1102 7 -0.8729362282278359 1102 8 -0.19407378004432374 1102 9 -0.4854241167879749 1102 10 -0.4007254383841641 1102 11 -0.7509984658212971 1102 12 -0.6347607405444009 1102 13 -0.6606657377562541 1102 14 -0.9722942334557203 1102 15 -0.44326150021122146 1102 16 -0.6948942379500522 1102 17 -0.8528581366104078 1102 18 -1.0488167339260173 1102 19 -0.665518520818992 1102 20 -0.8225934389409844 1102 21 -0.9022550739901902 1102 22 -1.0186447733852475 1102 23 -1.3248649731358952 1102 24 -4.245981115164444 1102 25 -2.2660706964951167 1103 1 -0.4855325168057938 1103 2 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1.002521616111732 2215 5 1.0708190105013422 2215 6 1.8572639882088993 2215 7 1.2653833928143001 2215 8 -0.13786335870301158 2215 9 0.6905768804695711 2215 10 0.5207205850513859 2215 11 0.4288320003966035 2215 12 0.35088872342966937 2215 13 0.46062571076031844 2215 14 0.7740832285238355 2215 15 0.8039486718157107 2215 16 0.8814607857348165 2215 17 0.43971229470348305 2215 18 -0.6514794115625193 2215 19 0.819512643479186 2215 20 -2.9021646181980287 2215 21 -1.540155846864785 2215 22 -0.5057452470825794 2215 23 -0.36658524239543056 2215 24 0.021024619066416506 2215 25 0.14101481833908736 2216 1 -0.2164578336753742 2216 2 -0.9204511496913454 2216 3 -0.1444426325546786 2216 4 -0.09835289721561224 2216 5 -0.23010941118180028 2216 6 -1.227527865639562 2216 7 -8.990471653606786 2216 8 -10.52694392174584 2216 9 -5.767182927856279 2216 10 -3.1686115503184844 2216 11 -2.102943980435981 2216 12 -1.6785092999306173 2216 13 -1.4307789013990089 2216 14 -1.6075220425153987 2216 15 -2.216698228453833 2216 16 -1.5670687141154431 2216 17 -1.7847261599236504 2216 18 -1.5878016637338277 2216 19 -0.772892268171006 2216 20 -1.7086515903453794 2216 21 -4.326145664175357 2216 22 -2.984589212341639 2216 23 -2.9127188153214547 2216 24 -2.8791970041740704 2216 25 -2.2750019531493777 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 43507.0 3 4.0 4 281.0 5 1725.0 6 2341.0 7 1847.0 8 1448.0 9 1328.0 10 1267.0 11 1245.0 12 1244.0 13 1215.0 14 1213.0 15 1309.0 16 1337.0 17 1505.0 18 1723.0 19 2194.0 20 2897.0 21 3573.0 22 4435.0 23 5218.0 24 6009.0 25 6973.0 26 7823.0 27 8702.0 28 9960.0 29 11553.0 30 13698.0 31 16627.0 32 21333.0 33 27729.0 34 37129.0 35 51540.0 36 73177.0 37 113617.0 38 153236.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.791495552194405 18.892286463967235 12.774934324821704 25.54128365901666 2 13.714640407487236 19.76768486489353 43.149853867292165 23.367820860327075 3 18.9769455595794 24.733381369916 29.217939480144363 27.071733590360243 4 11.997642935978899 16.75136715948843 38.84400975250828 32.406980152024396 5 12.45023846584119 37.970524032776005 36.92971918524301 12.6495183161398 6 30.943470307967008 37.585498783151714 19.239913870200773 12.231117038680507 7 28.265975755644885 30.892187270620354 22.49739971326568 18.344437260469082 8 24.03530662951933 39.11058777229972 20.739209130249417 16.11489646793153 9 25.907702854293905 15.772466658357223 20.476286925090363 37.84354356225851 10 14.325472389043503 29.670987225316253 35.04358476512222 20.95995562051803 11 34.06776597866188 22.685929554292553 24.85261491674145 18.393689550304117 12 23.261390140048515 24.842447366904054 32.33003401111476 19.566128481932665 13 29.030850868255172 22.423200002492745 25.64196042139899 22.90398870785309 14 19.920511146667998 23.169396012470184 27.718271062910237 29.19182177795158 15 22.54896309325539 32.33207812906533 23.046111229689917 22.07284754798936 16 21.049704130948932 28.57138405641299 29.437490457093535 20.941421355544545 17 21.087219692165792 29.391950317758813 28.4189648806009 21.101865109474495 18 20.796305759475526 28.193822990540053 30.77972903917341 20.230142210811014 19 23.818998546291954 27.027751333344757 27.140557831248337 22.012692289114952 20 23.034928884921175 28.207345364237895 28.41409245505549 20.343633295785445 21 22.260410029025156 27.32244097984442 27.176146711806524 23.2410022793239 22 22.377088513719844 29.12717108830183 27.520238562641392 20.975501835336935 23 21.65217973865883 28.585806809453402 28.0646513243893 21.69736212749847 24 23.312310899363407 27.404986180657907 28.510689346798497 20.772013573180192 25 22.157583386953792 27.992839526561212 28.471295008078123 21.378282078406873 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 188.0 1 188.0 2 236.5 3 285.0 4 285.0 5 285.0 6 409.5 7 534.0 8 534.0 9 534.0 10 803.5 11 1073.0 12 1073.0 13 1073.0 14 1925.0 15 2777.0 16 2777.0 17 2777.0 18 5031.0 19 7285.0 20 7285.0 21 7285.0 22 11747.5 23 16210.0 24 16210.0 25 16210.0 26 24090.0 27 31970.0 28 31970.0 29 31970.0 30 42694.5 31 53419.0 32 53419.0 33 53419.0 34 60164.0 35 66909.0 36 66909.0 37 66909.0 38 71934.5 39 76960.0 40 76960.0 41 76960.0 42 80086.5 43 83213.0 44 83213.0 45 83213.0 46 84521.5 47 85830.0 48 85830.0 49 85830.0 50 80967.5 51 76105.0 52 76105.0 53 76105.0 54 65905.5 55 55706.0 56 55706.0 57 55706.0 58 47203.5 59 38701.0 60 38701.0 61 38701.0 62 31240.5 63 23780.0 64 23780.0 65 23780.0 66 18036.0 67 12292.0 68 12292.0 69 12292.0 70 8866.0 71 5440.0 72 5440.0 73 5440.0 74 3803.5 75 2167.0 76 2167.0 77 2167.0 78 1466.5 79 766.0 80 766.0 81 766.0 82 500.5 83 235.0 84 235.0 85 235.0 86 160.5 87 86.0 88 86.0 89 86.0 90 53.5 91 21.0 92 21.0 93 21.0 94 13.0 95 5.0 96 5.0 97 5.0 98 5.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2039061502082678 2 0.1190101594798446 3 0.12212560868088765 4 0.07570541558534617 5 0.023521641467875047 6 0.021808144407301368 7 0.25671301416594755 8 0.2537533374249566 9 0.01900424012636262 10 0.034893031051682184 11 0.01651188076552818 12 0.017602287985893247 13 0.015577246005215262 14 0.01729074306578894 15 0.015733018465267413 16 0.019315785046466923 17 0.01916001258641477 18 0.015265701085110956 19 0.024144731308083655 20 0.017913832905997552 21 0.016356108305476024 22 0.01838115028615401 23 0.01853692274620616 24 0.016823425685632484 25 0.016356108305476024 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 641962.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.47716334743399 #Duplication Level Percentage of deduplicated Percentage of total 1 94.08273172009972 80.41925027411773 2 4.439456200903172 7.589442457167582 3 0.6956239022023138 1.7837987375077986 4 0.26356747811503267 0.9011600151963953 5 0.1341023453786383 0.5731344040601938 6 0.07663581119018935 0.39303670508201544 7 0.051535298029316265 0.30835637614673916 8 0.04023650009372638 0.27514415128323944 9 0.03139393653982929 0.24151181775605682 >10 0.15223718657802462 2.501851748572704 >50 0.016604886745780107 0.9760648481729554 >100 0.014050052041836961 2.5115631461094 >500 9.123410412221857E-4 0.5117331354602243 >1k 9.123410412221857E-4 1.0139521833669363 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1568 0.2442512173617753 No Hit GTCCTACAGTGTGCATTTCTCATTT 1375 0.21418713257170982 No Hit CTGTAGGACGTGGAATATGGCAAGA 1291 0.20110224592732903 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1244 0.19378094030487786 No Hit CTTTAGGACGTGAAATATGGCGAGG 1023 0.15935522663335214 No Hit CTGTAGGACCTGGAATATGGCGAGA 921 0.14346643570803255 No Hit GTATCAACGCAGAGTACTTTTTTTT 682 0.10623681775556808 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTTAAC 50 0.0014589903 13.346485 1 CCAACGA 65 5.5285087E-5 13.1319475 19 TGGACTG 80 1.9549734E-6 13.08561 5 ATAAGAC 90 5.1896313E-7 12.705963 3 TCTAGGA 130 1.8735591E-10 12.4616165 2 TCCAACG 100 1.4688158E-7 12.32944 18 GATATAC 250 0.0 12.202499 1 GTCTAGG 55 0.0029859834 12.133168 1 TAGGACC 325 0.0 12.011453 4 CTTATAC 80 2.7709253E-5 11.91184 3 TAAGACA 145 9.276846E-11 11.819511 4 ACTGTTC 255 0.0 11.560414 8 GTCCTAC 865 0.0 11.461885 1 GGACGTG 690 0.0 11.446859 6 CTAGGGC 75 2.0114594E-4 11.435367 3 AGGACGT 725 0.0 11.420169 5 TAGGACG 725 0.0 11.294199 4 TTAGGAC 465 0.0 11.271418 3 CCCTTTA 60 0.0057187662 11.122069 1 GTTTTAG 60 0.0057187662 11.122069 1 >>END_MODULE