FastQCFastQC Report
Thu 26 May 2016
SRR1926852_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926852_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641962
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA16420.2557783794056346No Hit
GTCCTACAGTGGACATTTCTAAATT15880.24736666656281833No Hit
GTCCTACAGTGTGCATTTCTCATTT13640.21247363551113616No Hit
GTCCTAAAGTGTGTATTTCTCATTT12430.19362516784482572No Hit
GTATCAACGCAGAGTACTTTTTTTT12190.18988662880357404No Hit
CTTTAGGACGTGAAATATGGCGAGG11940.18599231730227023No Hit
CTGTAGGACCTGGAATATGGCGAGA11180.17415361033830665No Hit
GGTATCAACGCAGAGTACTTTTTTT7440.11589471027880155No Hit
GTACATGGGAAGCAGTGGTATCAAC7260.11309080599786281No Hit
TATCAACGCAGAGTACTTTTTTTTT6830.10639259021562025No Hit
GATATACACTGTTCTACAAATCCCG6540.10187518887410782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3600.015.3431574
TATGTCG400.005317314.23270616
ATCTAGA500.001460054913.3451941
GTATTAG655.27218E-513.1985441
TGTAGGA9550.013.0756952
CTGTAGG9450.012.8105791
GTAGGAC9000.012.6977853
TCCAACG751.4939187E-512.65129418
GGACGTG7900.012.4165056
AGGACGT8250.012.3514815
GACGTGG4500.012.2295857
TTAGGAC5000.012.1898733
TGGCGAG8100.012.18272718
GGCGAGG3750.012.14524319
ATAGGAC1103.670175E-812.1206133
GGCGAGA4400.012.07623619
CTCTTAA959.949672E-712.0407771
TCTAGAG959.949672E-712.0407772
GTGTAGG1353.4924597E-1012.0036141
TAGGACG8800.011.9041744
Produced by FastQC (version 0.11.5)