FastQCFastQC Report
Thu 26 May 2016
SRR1926851_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926851_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences602596
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT8420.139728773506628No Hit
GTACATGGGAAGCAGTGGTATCAAC8420.139728773506628No Hit
CTGTAGGACGTGGAATATGGCAAGA7190.11931708806563603No Hit
CCCATGTACTCTGCGTTGATACCAC7180.11915113940351413No Hit
GTCCTAAAGTGTGTATTTCTCATTT6780.11251319291863868No Hit
GTATCAACGCAGAGTACTTTTTTTT6740.11184939827015114No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTAT400.00511238514.3170381
AGAACTA400.005209526514.2764895
TAGGACA1950.013.6709374
TCAGTAC500.001459335113.3458453
CCGTGCT500.001519105613.2749629
ACTGATC655.3937933E-513.1662338
GTCTTAG751.4055E-512.7262541
GTAGGAC4650.012.7103293
TGTAGGA5200.012.6502762
GGCGAGG1650.012.6438719
CTGTAGG4950.012.34061051
GACATGG1800.012.160447
GTCTAAG550.002958792312.1477891
TAAGGGT550.003014805512.1174224
TAGGACC2000.011.9010394
TATACTG1052.6444468E-711.7837685
GACGTGG1701.8189894E-1211.7561297
GGACATG2300.011.5857516
GGACAGT1251.774788E-811.420246
ATTTCTA3000.011.37948315
Produced by FastQC (version 0.11.5)