##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926851_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 602596 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.148658471015406 31.0 28.0 31.0 16.0 34.0 2 28.291297984055653 31.0 28.0 33.0 16.0 34.0 3 27.782708481304223 31.0 28.0 31.0 16.0 34.0 4 31.409211146439738 35.0 32.0 37.0 17.0 37.0 5 31.753698995678697 35.0 32.0 37.0 19.0 37.0 6 30.991247867559693 35.0 32.0 37.0 11.0 37.0 7 31.07651229015792 35.0 32.0 37.0 13.0 37.0 8 30.972957006020618 35.0 32.0 37.0 11.0 37.0 9 33.00940099170921 37.0 33.0 39.0 17.0 39.0 10 33.15478861459419 37.0 33.0 39.0 17.0 39.0 11 33.33795942887109 38.0 33.0 39.0 17.0 39.0 12 33.1885127017106 38.0 33.0 39.0 17.0 39.0 13 33.3130123664943 38.0 34.0 39.0 17.0 39.0 14 34.319592230947435 39.0 34.0 41.0 17.0 41.0 15 33.883178779812674 39.0 33.0 41.0 11.0 41.0 16 33.963876295229305 39.0 33.0 41.0 16.0 41.0 17 34.19879156184243 39.0 34.0 41.0 17.0 41.0 18 34.24271319424623 39.0 34.0 41.0 17.0 41.0 19 33.59670824233815 39.0 33.0 41.0 10.0 41.0 20 33.86766092041766 39.0 34.0 41.0 10.0 41.0 21 33.86056661511195 39.0 34.0 41.0 10.0 41.0 22 33.883777854482936 39.0 34.0 41.0 2.0 41.0 23 33.872858432515315 39.0 34.0 41.0 2.0 41.0 24 32.56380892007249 38.0 32.0 40.0 2.0 41.0 25 33.12338780874749 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.6218690682179471 1101 2 -0.6174469243794789 1101 3 -1.9265838837419622 1101 4 -1.2242130944542247 1101 5 -0.8269076227077399 1101 6 -3.2207935706523507 1101 7 -1.2868264783115748 1101 8 -0.7235202438863837 1101 9 -0.7291111706759921 1101 10 -9.461838228239273 1101 11 -4.429729217667415 1101 12 -2.183141581690961 1101 13 -1.484052027049735 1101 14 -1.2904774653292819 1101 15 -2.530180897541971 1101 16 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0.11212835977904234 1103 3 0.2553953176329209 1103 4 0.009231555699471983 1103 5 0.17419719464665917 1103 6 0.5071269927293436 1103 7 0.35470538477124336 1103 8 0.5499546196398626 1103 9 0.4306313893436524 1103 10 0.33275092250440963 1103 11 0.3395242915682104 1103 12 0.2486929412208454 1103 13 0.40167954492248015 1103 14 0.37291525089299427 1103 15 0.7379602785749313 1103 16 0.5071323019329128 1103 17 0.40233605224849356 1103 18 0.28978502256804717 1103 19 -1.5336138500681358 1103 20 -0.32446066527722195 1103 21 0.08505085785948552 1103 22 0.20260453519752986 1103 23 0.19410860151292297 1103 24 1.6246910180969323 1103 25 0.8694905669916508 1104 1 -0.6084204181733739 1104 2 -0.3029146349553926 1104 3 -1.7547547829093695 1104 4 -0.8727578541738303 1104 5 -0.4650157730962263 1104 6 -1.4414907351624144 1104 7 -0.39172892841921936 1104 8 0.3862255577559601 1104 9 0.1518834235427775 1104 10 0.31056770446284787 1104 11 0.2959789098762471 1104 12 0.36277279490600023 1104 13 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-1.6179057388136577 2214 25 -0.8706785083117552 2215 1 0.43227104854045706 2215 2 -0.11911196105701904 2215 3 0.1834186864115459 2215 4 0.6536044780524755 2215 5 0.37234936927805506 2215 6 1.2314610992943216 2215 7 0.7165436493838868 2215 8 -0.758929393410348 2215 9 -0.041260208426095346 2215 10 -0.27049753158303247 2215 11 -0.42210887940851194 2215 12 -0.5529555181536523 2215 13 -0.17564361964368658 2215 14 -0.18533927229184144 2215 15 -0.34769109749101546 2215 16 0.09826899651081789 2215 17 -0.42453275611563157 2215 18 -1.2080340676621404 2215 19 0.15905080311213737 2215 20 -3.923910150780781 2215 21 -2.117796881594497 2215 22 -1.4402648811777965 2215 23 -1.1986247833691834 2215 24 -1.2642863555149404 2215 25 -0.9079442865899949 2216 1 -0.20054948867641187 2216 2 -0.8967513149378767 2216 3 0.1196739863288876 2216 4 -0.0654478699670058 2216 5 -0.2988357308926126 2216 6 -1.3106042381064924 2216 7 -9.678229081042346 2216 8 -10.577531979393392 2216 9 -5.528607801733383 2216 10 -2.817775073808928 2216 11 -2.074455114906211 2216 12 -1.6516534084941767 2216 13 -1.4561987952768511 2216 14 -1.322852676944862 2216 15 -2.853695192724011 2216 16 -1.5340569808643068 2216 17 -1.691767522384069 2216 18 -1.3234548201463383 2216 19 -0.8834516102209591 2216 20 -1.8733490880024064 2216 21 -4.822639801856386 2216 22 -2.9865711435541797 2216 23 -2.7779624894440467 2216 24 -3.269580821796662 2216 25 -2.3912491752991656 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36356.0 3 1.0 4 244.0 5 1533.0 6 2034.0 7 1652.0 8 1327.0 9 1111.0 10 1079.0 11 1132.0 12 1123.0 13 1105.0 14 1122.0 15 1199.0 16 1265.0 17 1324.0 18 1644.0 19 1966.0 20 2596.0 21 3304.0 22 4044.0 23 4820.0 24 5647.0 25 6510.0 26 7334.0 27 8063.0 28 8800.0 29 10787.0 30 12941.0 31 15528.0 32 19906.0 33 25483.0 34 34946.0 35 47934.0 36 69055.0 37 108847.0 38 148834.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.73832646157275 18.268016957919546 12.54629914528493 25.447357435222777 2 14.312017468351906 19.765559828108227 41.74158404982602 24.180838653713852 3 19.3863917361305 24.491817227030406 28.958607532671266 27.163183504167833 4 12.284463073706446 16.60093098334493 38.05606160499137 33.05854433795725 5 13.022051439526603 37.66306197143356 36.23888921164236 13.075997377397483 6 31.673768023808773 36.75504218488316 18.901450203911583 12.669739587396489 7 28.28242978706404 31.098937317726023 22.095767914452747 18.522864980757195 8 24.7322153727349 37.903091589332554 20.53886455323738 16.82582848469517 9 26.161935713029017 15.607665572312055 19.8308342434513 38.39956447120763 10 14.605767937861783 29.559478020381842 34.45473847068139 21.380015571074985 11 34.70946540306385 22.803438947071417 23.72408756701134 18.763008082853396 12 22.827423779956483 24.897468709491637 31.92420236382135 20.350905146730533 13 29.022058982176556 21.864300165471413 25.414051958354978 23.699588893997056 14 20.759004149377596 22.257095435684647 27.45128630705394 29.53261410788382 15 23.567135865046133 30.463704758537936 23.061755933857466 22.907403442558465 16 22.105002829974158 28.19517360771081 27.947530121381824 21.752293440933208 17 21.84150337103269 28.452611346985655 27.655248420710194 22.050636861271467 18 21.64444038737625 28.131249844402213 28.97969345161531 21.244616316606226 19 23.97704015151641 27.051023914295623 26.80734940898973 22.164586525198235 20 23.22842029613327 27.93050278921625 27.73498379250422 21.10609312214626 21 23.100823055512677 27.001664699897592 26.71652467755775 23.18098756703197 22 22.748178011322803 27.969912083133476 27.21041126902694 22.071498636516786 23 22.72325155644962 27.435339497720307 27.151352795800115 22.690056150029957 24 23.359230681137312 27.34822997907078 27.475697377789803 21.8168419620021 25 23.003332757956795 27.299569795387253 27.37857342025945 22.318524026396506 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 214.0 1 214.0 2 259.0 3 304.0 4 304.0 5 304.0 6 431.0 7 558.0 8 558.0 9 558.0 10 782.0 11 1006.0 12 1006.0 13 1006.0 14 1723.5 15 2441.0 16 2441.0 17 2441.0 18 4361.5 19 6282.0 20 6282.0 21 6282.0 22 10101.5 23 13921.0 24 13921.0 25 13921.0 26 20379.5 27 26838.0 28 26838.0 29 26838.0 30 35547.0 31 44256.0 32 44256.0 33 44256.0 34 50396.0 35 56536.0 36 56536.0 37 56536.0 38 61765.5 39 66995.0 40 66995.0 41 66995.0 42 70974.5 43 74954.0 44 74954.0 45 74954.0 46 77609.0 47 80264.0 48 80264.0 49 80264.0 50 77313.5 51 74363.0 52 74363.0 53 74363.0 54 66660.0 55 58957.0 56 58957.0 57 58957.0 58 50646.0 59 42335.0 60 42335.0 61 42335.0 62 34642.5 63 26950.0 64 26950.0 65 26950.0 66 20831.0 67 14712.0 68 14712.0 69 14712.0 70 10684.5 71 6657.0 72 6657.0 73 6657.0 74 4645.5 75 2634.0 76 2634.0 77 2634.0 78 1820.0 79 1006.0 80 1006.0 81 1006.0 82 652.0 83 298.0 84 298.0 85 298.0 86 196.0 87 94.0 88 94.0 89 94.0 90 56.0 91 18.0 92 18.0 93 18.0 94 10.0 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22286905322969286 2 0.13640980026419028 3 0.14006067083087176 4 0.07251956534726417 5 0.02339876135918592 6 0.02140737741372329 7 0.3073369222497328 8 0.2887506720920816 9 0.018254352833407456 10 0.03269188643801154 11 0.014271584942482195 12 0.01609702022582294 13 0.012446149659141448 14 0.015931071563701053 15 0.013109944307628994 16 0.01941599346826066 17 0.018254352833407456 18 0.013441841631872765 19 0.025390145304648552 20 0.01609702022582294 21 0.014105636280360307 22 0.01576512290157917 23 0.017092712198554256 24 0.014769430928847851 25 0.015267276915213509 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 602596.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.56581498342587 #Duplication Level Percentage of deduplicated Percentage of total 1 92.91731713238883 79.50545966506289 2 5.392418089950728 9.228132971960054 3 0.8957716681192494 2.299422984650594 4 0.28382961179900595 0.9714444820004532 5 0.14083115459066914 0.6025166258803721 6 0.0745401220624799 0.3826851775944082 7 0.05434446045423654 0.32550196340209153 8 0.03320444964670824 0.2272932636077374 9 0.031032322197904905 0.23897753457227913 >10 0.14877250612340978 2.3390349057295103 >50 0.014163333796041887 0.8141275850407045 >100 0.011640970876927456 1.8646414679398322 >500 0.00213417799381617 1.2007613725591157 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 842 0.139728773506628 No Hit GTACATGGGAAGCAGTGGTATCAAC 842 0.139728773506628 No Hit CTGTAGGACGTGGAATATGGCAAGA 719 0.11931708806563603 No Hit CCCATGTACTCTGCGTTGATACCAC 718 0.11915113940351413 No Hit GTCCTAAAGTGTGTATTTCTCATTT 678 0.11251319291863868 No Hit GTATCAACGCAGAGTACTTTTTTTT 674 0.11184939827015114 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.6594866212188598E-4 0.0 5 0.0 0.0 0.0 1.6594866212188598E-4 0.0 6 0.0 0.0 0.0 1.6594866212188598E-4 0.0 7 0.0 0.0 0.0 1.6594866212188598E-4 0.0 8 0.0 0.0 0.0 1.6594866212188598E-4 0.0 9 0.0 0.0 0.0 1.6594866212188598E-4 0.0 10 0.0 0.0 0.0 1.6594866212188598E-4 0.0 11 0.0 0.0 0.0 1.6594866212188598E-4 0.0 12 0.0 0.0 0.0 1.6594866212188598E-4 1.6594866212188598E-4 13 0.0 0.0 0.0 1.6594866212188598E-4 3.3189732424377197E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCCTAT 40 0.005112385 14.317038 1 AGAACTA 40 0.0052095265 14.276489 5 TAGGACA 195 0.0 13.670937 4 TCAGTAC 50 0.0014593351 13.345845 3 CCGTGCT 50 0.0015191056 13.274962 9 ACTGATC 65 5.3937933E-5 13.166233 8 GTCTTAG 75 1.4055E-5 12.726254 1 GTAGGAC 465 0.0 12.710329 3 TGTAGGA 520 0.0 12.650276 2 GGCGAGG 165 0.0 12.64387 19 CTGTAGG 495 0.0 12.3406105 1 GACATGG 180 0.0 12.16044 7 GTCTAAG 55 0.0029587923 12.147789 1 TAAGGGT 55 0.0030148055 12.117422 4 TAGGACC 200 0.0 11.901039 4 TATACTG 105 2.6444468E-7 11.783768 5 GACGTGG 170 1.8189894E-12 11.756129 7 GGACATG 230 0.0 11.585751 6 GGACAGT 125 1.774788E-8 11.42024 6 ATTTCTA 300 0.0 11.379483 15 >>END_MODULE