FastQCFastQC Report
Thu 26 May 2016
SRR1926851_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926851_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences602596
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT11540.1915047560886564No Hit
GTACATGGGAAGCAGTGGTATCAAC9190.1525068204900132No Hit
GTCCTACAGTGGACATTTCTAAATT8730.14487318203240646No Hit
CCCATGTACTCTGCGTTGATACCAC8330.138235235547531No Hit
CTGTAGGACGTGGAATATGGCAAGA8020.13309082702175254No Hit
GGTATCAACGCAGAGTACTTTTTTT7190.11931708806563603No Hit
CTTTAGGACGTGAAATATGGCGAGG6940.11516837151258888No Hit
GATATACACTGTTCTACAAATCCCG6900.11450457686410132No Hit
GTCCTAAAGTGTGTATTTCTCATTT6800.11284509024288246No Hit
TATCAACGCAGAGTACTTTTTTTTT6780.11251319291863868No Hit
GTCCTACAGTGTGCATTTCTCATTT6290.10438170847466628No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTAAG400.00516640514.2943831
TAGGACC2000.014.2824784
GCCTAAG500.001462986313.3414241
TATAACT655.3266238E-513.1838264
CCAACGA655.499782E-513.13896519
CTAGGAC801.9414492E-613.0933633
TGCCGGT802.0209918E-613.04663910
CTCCTAT905.187103E-712.7061191
TCCTATA1051.9021172E-812.7050592
TCCAACG1155.3860276E-912.37728618
AGGACGT3300.012.1184665
TGTAGGA5150.012.0266332
TGTAGAA2800.011.9109922
GGCGAGG2350.011.71014119
ATTTAGA1552.3646862E-1111.6814311
GACGTGG1950.011.6781117
TCTAGGA907.192655E-611.6463042
CCTACAC907.24625E-611.6385453
TAGGACT907.2529747E-611.6375754
GTAGGAC5200.011.5368093
Produced by FastQC (version 0.11.5)