##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926851_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 602596 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.91511062137817 31.0 30.0 33.0 26.0 34.0 2 30.258556313019003 31.0 30.0 34.0 26.0 34.0 3 30.34033415422605 31.0 30.0 34.0 26.0 34.0 4 33.95059044533983 37.0 35.0 37.0 30.0 37.0 5 33.92716015373484 37.0 35.0 37.0 30.0 37.0 6 33.88130521941732 37.0 35.0 37.0 30.0 37.0 7 33.87718139516359 37.0 35.0 37.0 30.0 37.0 8 33.9203844698604 37.0 35.0 37.0 30.0 37.0 9 35.47767990494461 39.0 35.0 39.0 30.0 39.0 10 35.37128357971178 39.0 35.0 39.0 30.0 39.0 11 35.41843789205372 39.0 35.0 39.0 30.0 39.0 12 35.30275010122868 39.0 35.0 39.0 30.0 39.0 13 35.3323686184442 39.0 35.0 39.0 30.0 39.0 14 36.425140558516816 40.0 37.0 41.0 30.0 41.0 15 36.40661239039091 40.0 36.0 41.0 30.0 41.0 16 36.38053189865183 40.0 36.0 41.0 30.0 41.0 17 36.36250157651229 40.0 36.0 41.0 30.0 41.0 18 36.340651116170704 40.0 36.0 41.0 30.0 41.0 19 36.40815737243526 40.0 37.0 41.0 30.0 41.0 20 36.36853712935366 40.0 37.0 41.0 30.0 41.0 21 36.31881227223546 40.0 37.0 41.0 30.0 41.0 22 36.23284588679646 40.0 37.0 41.0 29.0 41.0 23 36.16987500746769 40.0 37.0 41.0 29.0 41.0 24 36.10240525990879 40.0 37.0 41.0 29.0 41.0 25 35.985837941174516 40.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.23110702484570922 1101 2 -0.138460714896965 1101 3 0.1500026720238381 1101 4 0.062742272694706 1101 5 0.008767682387464504 1101 6 -0.009327997443158154 1101 7 0.13861516098589277 1101 8 0.07431602675885074 1101 9 0.10715640121379266 1101 10 0.11468976221554072 1101 11 0.2927404561141316 1101 12 0.23702438558888161 1101 13 0.23715573694743597 1101 14 0.282075304574505 1101 15 0.3479045243097403 1101 16 0.2900912098419255 1101 17 0.338222364682359 1101 18 0.15100347696452587 1101 19 0.1685144851480942 1101 20 0.2572213464730524 1101 21 0.06921868180741342 1101 22 0.010525325249190587 1101 23 0.25960789847238175 1101 24 0.208396528291658 1101 25 0.08311649582376646 1102 1 -0.5022963404669696 1102 2 -0.5944751416650469 1102 3 -0.22029925452105914 1102 4 -0.1277874825144636 1102 5 -0.34334008985528897 1102 6 -0.4823760092988181 1102 7 -0.31153392752090525 1102 8 -0.19096331332363548 1102 9 -0.07868643985964496 1102 10 -0.2168312976519715 1102 11 -0.2320867976220029 1102 12 -0.22235569190142712 1102 13 -0.186818052043229 1102 14 -0.4482084099611754 1102 15 -0.3003269467563783 1102 16 -0.4949267343436219 1102 17 -0.3557087510710346 1102 18 -0.45159084160279406 1102 19 -0.593122453091766 1102 20 -0.520949239346578 1102 21 -0.5205022459337627 1102 22 -0.3763370312419596 1102 23 -0.3264259901486781 1102 24 -0.5848971671392746 1102 25 -0.47401029189919797 1103 1 -0.5575943319724068 1103 2 -0.5705570369324349 1103 3 -0.4695556014547826 1103 4 -0.29910545290974966 1103 5 -0.7276256526327387 1103 6 -0.9518900212881718 1103 7 -0.6731437533369018 1103 8 -0.7063414021983832 1103 9 -0.5935721268738732 1103 10 -0.4951393543564677 1103 11 -0.4829390316113731 1103 12 -0.4611049406729464 1103 13 -0.5069575406523299 1103 14 -0.7995358748743868 1103 15 -0.488134066634764 1103 16 -0.5650674855215954 1103 17 -0.5363520551632703 1103 18 -0.44199784330474046 1103 19 -0.47340819767913445 1103 20 -0.6158110890604789 1103 21 -0.3558203375356612 1103 22 -0.547713079805412 1103 23 -0.7649010089609547 1103 24 -0.6379023335711054 1103 25 -0.6493886837904768 1104 1 -0.6645329790517636 1104 2 -0.48937835681424247 1104 3 -0.28293534648543783 1104 4 -0.2936432287864861 1104 5 -0.5084975435652481 1104 6 -0.4309801750269173 1104 7 -0.6560023992363 1104 8 -0.4588598759221867 1104 9 -0.49982443148013544 1104 10 -0.4909824592203549 1104 11 -0.4074708585311413 1104 12 -0.47853145138844866 1104 13 -0.5915949738494817 1104 14 -0.4473674554778384 1104 15 -0.4156428302841775 1104 16 -0.47393290634884977 1104 17 -0.5470183875083876 1104 18 -0.6244144964510454 1104 19 -0.5128188084551013 1104 20 -0.40536304902055775 1104 21 -0.45391667130655833 1104 22 -0.41479568181035376 1104 23 -0.49930220465214603 1104 24 -0.5185593737437699 1104 25 -0.6359691151636042 1105 1 0.26342206810858215 1105 2 0.06775324001256067 1105 3 0.22597688280499284 1105 4 0.3159317357314322 1105 5 -0.13918767313833058 1105 6 -0.12081597820350254 1105 7 -0.38043414186001456 1105 8 -0.3627934911779107 1105 9 -0.31016150524176567 1105 10 -0.2285057008926401 1105 11 -0.03419690782161666 1105 12 0.05507437468526177 1105 13 0.062015252728116366 1105 14 0.02384430305539098 1105 15 0.06090151688172796 1105 16 0.03618099900162974 1105 17 0.007465653411465212 1105 18 0.20572363584937392 1105 19 0.012409135228118373 1105 20 0.3076539799623248 1105 21 -0.10036157192045181 1105 22 -0.22291195182720713 1105 23 -0.19350345275110215 1105 24 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2210 1 0.2905435952301083 2210 2 0.25728399580837547 2210 3 0.2629013715367101 2210 4 0.311695171253902 2210 5 0.15308176370942306 2210 6 0.25970516135378574 2210 7 0.33542229086388886 2210 8 0.28611816375784116 2210 9 0.19487204798455338 2210 10 0.22121114039046574 2210 11 -0.2500977442043819 2210 12 0.08535311580737925 2210 13 0.12458911650679738 2210 14 -0.11292807468083765 2210 15 -0.10516475159418093 2210 16 0.13508160995043994 2210 17 0.062078738145025625 2210 18 0.14307918163865452 2210 19 -0.05516298656087315 2210 20 -0.10819425516302061 2210 21 -0.006550303410392644 2210 22 0.06807205602427047 2210 23 -0.10579484094610336 2210 24 -0.03083604164957876 2210 25 0.0036934715188507994 2211 1 0.11852696321347622 2211 2 0.19305669158107364 2211 3 0.23852160715694737 2211 4 0.20077348033220943 2211 5 0.3214532120808684 2211 6 0.2605090421576648 2211 7 0.2343966498382457 2211 8 0.1570952947349724 2211 9 0.09258514569765453 2211 10 0.24678286596218868 2211 11 0.21716490305826852 2211 12 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2212 24 0.3558160459180755 2212 25 0.2964964866006028 2213 1 0.21265227338717452 2213 2 0.10377526064641529 2213 3 0.06135762866232142 2213 4 0.17533820324371163 2213 5 0.24992302184100623 2213 6 0.10264200328256123 2213 7 0.1572275017820033 2213 8 0.028602738903707348 2213 9 0.11508824743462753 2213 10 0.07945966496963308 2213 11 0.02149286004751616 2213 12 0.04820562402279194 2213 13 -0.08710652764852256 2213 14 -0.07472850724739999 2213 15 -0.20188864670517148 2213 16 -0.5293586522720872 2213 17 -0.2523787093769343 2213 18 -0.22870800567272198 2213 19 -0.046538900315816534 2213 20 -0.2382157158296394 2213 21 -0.1315607822515119 2213 22 -0.19441453105909545 2213 23 -0.08603803389090814 2213 24 -0.1848121331256678 2213 25 0.08610326965445836 2214 1 0.48334638387026274 2214 2 0.43524697628829756 2214 3 0.5490217684993155 2214 4 0.629994817239897 2214 5 0.4990876847395427 2214 6 0.5707967280116648 2214 7 0.3764156634158269 2214 8 0.454290916151308 2214 9 0.35878516059079146 2214 10 0.279235021744455 2214 11 0.3644124022515527 2214 12 0.4947957701677481 2214 13 0.3403313685510838 2214 14 0.42864894801912357 2214 15 0.34093213835556213 2214 16 0.3366351256375424 2214 17 0.5890594756775585 2214 18 0.4576414540905702 2214 19 0.39296259079994655 2214 20 0.43173244642873243 2214 21 0.4680951291246842 2214 22 0.345695311866308 2214 23 0.3485295035040892 2214 24 0.35687470133706256 2214 25 0.4968295445193931 2215 1 0.7670638269432182 2215 2 0.4492143420413939 2215 3 0.260638323279597 2215 4 0.43942219067231747 2215 5 0.46449817898340484 2215 6 0.3273765762062055 2215 7 0.2341136094365126 2215 8 0.3914162260291931 2215 9 0.11385882750546017 2215 10 0.40873980121289577 2215 11 0.11279299405727983 2215 12 0.3184337198078637 2215 13 0.41848618636825563 2215 14 0.4371479086295622 2215 15 0.312621100909773 2215 16 0.329242102330511 2215 17 0.41011211733567876 2215 18 0.4287424984592434 2215 19 0.34672292125975446 2215 20 0.39460550876434297 2215 21 0.35215364134696614 2215 22 0.5098805616309932 2215 23 0.38092915764732993 2215 24 0.5057788658852118 2215 25 0.416742448366044 2216 1 -0.28913759667553407 2216 2 -0.13082902857719247 2216 3 -2.0853127281803943 2216 4 -1.5717911360801011 2216 5 -0.6849143949280148 2216 6 -0.3418659642654376 2216 7 -0.3358433172754687 2216 8 -0.27174392216036125 2216 9 -0.4195313950762056 2216 10 -0.48654532888905067 2216 11 -0.31091792927306017 2216 12 -0.34071940345151575 2216 13 -0.24595446545963284 2216 14 -0.15694334013899436 2216 15 -0.18606668019150163 2216 16 -0.308956153726605 2216 17 -0.16189936358858148 2216 18 -0.23445134719447935 2216 19 -0.11401457747658839 2216 20 -0.3123801737777967 2216 21 0.012475273731418213 2216 22 -0.32297406949079743 2216 23 -0.47842159132836315 2216 24 -0.5331337764122424 2216 25 -0.4324782482200007 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13356.0 3 11.0 4 206.0 5 1385.0 6 1900.0 7 1432.0 8 1110.0 9 961.0 10 892.0 11 800.0 12 735.0 13 710.0 14 674.0 15 725.0 16 720.0 17 778.0 18 841.0 19 1028.0 20 1357.0 21 1702.0 22 2176.0 23 2707.0 24 3280.0 25 3935.0 26 4689.0 27 5117.0 28 5904.0 29 7252.0 30 9476.0 31 12509.0 32 15963.0 33 21273.0 34 28875.0 35 40722.0 36 59802.0 37 103872.0 38 243721.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.154693195373426 19.098741680705217 10.62261386178092 28.12395126214044 2 14.240193513123772 20.66476941402804 39.932682419512126 25.162354653336056 3 18.77638951589346 24.282669888732507 29.46729691690788 27.473643678466154 4 12.184614567637356 17.26463501251253 37.526468811608446 33.02428160824167 5 13.143583539528993 38.37645602348556 35.19996415431567 13.279996282669773 6 32.23883828206964 37.52085796599444 17.43476161135073 12.805542140585189 7 28.26688089017836 31.67592276003094 21.167794770334915 18.88940157945579 8 25.320846340617415 37.945919571419324 19.535239574672183 17.19799451329108 9 26.16029063485926 15.78011859734492 19.525577427669063 38.53401334012676 10 14.713164812405404 29.8403852792013 33.865603674889144 21.580846233504154 11 34.86119777491603 22.65260942872695 23.695949444392813 18.790243351964207 12 23.004339564715938 24.980957359420504 31.599332885271203 20.415370190592355 13 29.26514369542345 21.809377458122718 25.42010043246365 23.505378413990183 14 21.018926476323234 22.188700536844813 27.10858868726093 29.68378429957102 15 23.151614723356236 30.662816555478113 23.186464867735403 22.99910385343025 16 22.391564455611 28.299532188392497 27.3916225379319 21.917280818064597 17 21.87568453823125 28.561590186459917 27.490225623801024 22.07249965150781 18 21.70101262209713 27.994968419864787 28.889932525050032 21.414086432988046 19 23.46156782178382 27.03594791849902 27.092868498524712 22.409615761192445 20 23.2424260527853 27.75128113217567 27.67428044182465 21.332012373214383 21 23.00011783782528 26.851755036737185 27.045440588989965 23.10268653644757 22 22.97160950288419 27.851495861240366 26.9198600720881 22.257034563787347 23 22.566068420746937 27.704677270803774 27.069258789070606 22.659995519378686 24 22.98604703648879 27.233171146174218 27.700980424695814 22.079801392641173 25 23.005139728591793 27.252842450805808 27.477384032487993 22.2646337881144 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 158.0 1 158.0 2 172.0 3 186.0 4 186.0 5 186.0 6 311.0 7 436.0 8 436.0 9 436.0 10 707.5 11 979.0 12 979.0 13 979.0 14 1551.0 15 2123.0 16 2123.0 17 2123.0 18 3954.5 19 5786.0 20 5786.0 21 5786.0 22 9601.5 23 13417.0 24 13417.0 25 13417.0 26 19722.0 27 26027.0 28 26027.0 29 26027.0 30 34864.5 31 43702.0 32 43702.0 33 43702.0 34 49590.0 35 55478.0 36 55478.0 37 55478.0 38 60841.0 39 66204.0 40 66204.0 41 66204.0 42 70401.0 43 74598.0 44 74598.0 45 74598.0 46 77725.5 47 80853.0 48 80853.0 49 80853.0 50 78479.0 51 76105.0 52 76105.0 53 76105.0 54 67958.0 55 59811.0 56 59811.0 57 59811.0 58 51479.5 59 43148.0 60 43148.0 61 43148.0 62 35323.5 63 27499.0 64 27499.0 65 27499.0 66 21321.5 67 15144.0 68 15144.0 69 15144.0 70 11007.0 71 6870.0 72 6870.0 73 6870.0 74 4789.0 75 2708.0 76 2708.0 77 2708.0 78 1821.5 79 935.0 80 935.0 81 935.0 82 620.0 83 305.0 84 305.0 85 305.0 86 201.5 87 98.0 88 98.0 89 98.0 90 61.0 91 24.0 92 24.0 93 24.0 94 13.0 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11317698756712624 2 0.11168344960802927 3 0.013939687618238421 4 0.0 5 0.0021573326075845175 6 0.34948788242869183 7 0.018254352833407456 8 0.008131484443972412 9 0.008795279092459956 10 0.0033189732424377194 11 0.0019913839454626316 12 1.6594866212188598E-4 13 3.3189732424377197E-4 14 1.6594866212188598E-4 15 0.0026551785939501757 16 4.978459863656579E-4 17 0.0 18 3.3189732424377197E-4 19 3.3189732424377197E-4 20 6.637946484875439E-4 21 0.012114252334897676 22 0.0 23 1.6594866212188598E-4 24 0.0 25 0.005808203174266009 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 602596.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.41798758410621 #Duplication Level Percentage of deduplicated Percentage of total 1 90.81927502470784 74.85141881383898 2 6.98125097725639 11.50761312730093 3 1.182946299220183 2.924881602053803 4 0.39925437964812277 1.3162297001895604 5 0.17259119909265402 0.7112309651972182 6 0.10624901593556985 0.525409804572078 7 0.06289281476745394 0.36284494586434574 8 0.041277708660037694 0.27216205438746727 9 0.032744056333680716 0.24288293055263147 >10 0.1712745534432366 2.686565311386053 >50 0.014517141419911374 0.8430145357255692 >100 0.013307317281928386 2.2269260186094106 >500 0.0022178862135103393 1.337052962101104 >1k 2.0162601941003084E-4 0.19176722822081457 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1154 0.1915047560886564 No Hit GTACATGGGAAGCAGTGGTATCAAC 919 0.1525068204900132 No Hit GTCCTACAGTGGACATTTCTAAATT 873 0.14487318203240646 No Hit CCCATGTACTCTGCGTTGATACCAC 833 0.138235235547531 No Hit CTGTAGGACGTGGAATATGGCAAGA 802 0.13309082702175254 No Hit GGTATCAACGCAGAGTACTTTTTTT 719 0.11931708806563603 No Hit CTTTAGGACGTGAAATATGGCGAGG 694 0.11516837151258888 No Hit GATATACACTGTTCTACAAATCCCG 690 0.11450457686410132 No Hit GTCCTAAAGTGTGTATTTCTCATTT 680 0.11284509024288246 No Hit TATCAACGCAGAGTACTTTTTTTTT 678 0.11251319291863868 No Hit GTCCTACAGTGTGCATTTCTCATTT 629 0.10438170847466628 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 3.3189732424377197E-4 13 0.0 0.0 0.0 0.0 4.978459863656579E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATTAAG 40 0.005166405 14.294383 1 TAGGACC 200 0.0 14.282478 4 GCCTAAG 50 0.0014629863 13.341424 1 TATAACT 65 5.3266238E-5 13.183826 4 CCAACGA 65 5.499782E-5 13.138965 19 CTAGGAC 80 1.9414492E-6 13.093363 3 TGCCGGT 80 2.0209918E-6 13.046639 10 CTCCTAT 90 5.187103E-7 12.706119 1 TCCTATA 105 1.9021172E-8 12.705059 2 TCCAACG 115 5.3860276E-9 12.377286 18 AGGACGT 330 0.0 12.118466 5 TGTAGGA 515 0.0 12.026633 2 TGTAGAA 280 0.0 11.910992 2 GGCGAGG 235 0.0 11.710141 19 ATTTAGA 155 2.3646862E-11 11.681431 1 GACGTGG 195 0.0 11.678111 7 TCTAGGA 90 7.192655E-6 11.646304 2 CCTACAC 90 7.24625E-6 11.638545 3 TAGGACT 90 7.2529747E-6 11.637575 4 GTAGGAC 520 0.0 11.536809 3 >>END_MODULE