FastQCFastQC Report
Thu 26 May 2016
SRR1926850_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926850_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629915
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12530.19891572672503435No Hit
GTCCTACAGTGTGCATTTCTCATTT10210.16208536072327218No Hit
GTCCTAAAGTGTGTATTTCTCATTT9190.14589269980870434No Hit
CTGTAGGACGTGGAATATGGCAAGA9080.14414643245517253No Hit
GTATCAACGCAGAGTACTTTTTTTT8570.1360501019978886No Hit
GTACATGGGAAGCAGTGGTATCAAC8290.13160505782526213No Hit
CCCATGTACTCTGCGTTGATACCAC7350.11668240953144472No Hit
CTTTAGGACGTGAAATATGGCGAGG7340.11652365795385092No Hit
CTGTAGGACCTGGAATATGGCGAGA6470.10271227070319013No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG551.1445434E-515.51894918
TGTACCG508.5745094E-515.2260695
CCAACGA551.9857756E-413.794620519
GTGTAGA1009.509677E-913.3504661
TAGGACC2950.013.2334164
TAGGACT905.2413816E-712.6954734
TAAGACT604.0084304E-412.6954714
CTACACT1551.8189894E-1212.285944
AGGACCT4300.012.1720035
CTGTAGG8150.012.0516931
GTCCTAT959.90125E-712.0455341
TGTAGGA8500.011.9984532
GTAGGAC8250.011.898963
GGACCTG4300.011.5080766
CTTAGAC752.0088945E-411.4368663
TAGAGTG752.0373744E-411.4195535
GTCCTAC7350.011.417311
GTGCTAA855.1098705E-511.218881
GTCTTAG855.1098705E-511.218881
TCCTACA7850.011.1706532
Produced by FastQC (version 0.11.5)