##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926850_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 629915 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.195689894668327 31.0 28.0 31.0 16.0 34.0 2 28.344462348094584 31.0 28.0 31.0 16.0 34.0 3 27.810991959232595 31.0 28.0 31.0 16.0 34.0 4 31.44597286935539 35.0 32.0 37.0 18.0 37.0 5 31.81870093584055 35.0 32.0 37.0 19.0 37.0 6 31.03056920378146 35.0 32.0 37.0 13.0 37.0 7 31.121719597088497 35.0 32.0 37.0 15.0 37.0 8 31.004435519077973 35.0 32.0 37.0 11.0 37.0 9 33.06932363890366 37.0 33.0 39.0 17.0 39.0 10 33.23796226475 37.0 33.0 39.0 17.0 39.0 11 33.407655001071575 38.0 34.0 39.0 18.0 39.0 12 33.27204146591207 38.0 33.0 39.0 17.0 39.0 13 33.377311224530295 38.0 34.0 39.0 17.0 39.0 14 34.40274005222927 39.0 34.0 41.0 17.0 41.0 15 33.985745695847854 39.0 33.0 41.0 11.0 41.0 16 34.062090917028485 39.0 33.0 41.0 17.0 41.0 17 34.28357159299271 39.0 34.0 41.0 17.0 41.0 18 34.322138701253344 39.0 34.0 41.0 17.0 41.0 19 33.67951866521673 39.0 33.0 41.0 10.0 41.0 20 33.961086813300206 39.0 34.0 41.0 10.0 41.0 21 33.95429700832652 39.0 34.0 41.0 10.0 41.0 22 33.97625076399197 39.0 34.0 41.0 10.0 41.0 23 33.9630140574522 39.0 34.0 41.0 2.0 41.0 24 32.65104498225951 38.0 32.0 40.0 2.0 41.0 25 33.21566719319273 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.5971662146039165 1101 2 -0.6310541726154604 1101 3 -1.6837759228852 1101 4 -1.0021406014108933 1101 5 -0.7940214149667533 1101 6 -2.646383289356816 1101 7 -1.2277211204721716 1101 8 -0.5047984071938814 1101 9 -0.40726249316202967 1101 10 -8.924823314380351 1101 11 -4.199625281413951 1101 12 -2.132601158991516 1101 13 -1.048396561713787 1101 14 -0.9130844433379366 1101 15 -2.4975075099261836 1101 16 -0.7339314115415689 1101 17 -0.48406914241821397 1101 18 -0.2548716810630438 1101 19 -1.0557982631901055 1101 20 -0.44754108817385685 1101 21 -0.37672707432884067 1101 22 -0.29249358732500497 1101 23 -0.35021678504793385 1101 24 -0.11957251557867465 1101 25 -0.13719592233522349 1102 1 -0.652682366369131 1102 2 -0.9404292436559487 1102 3 -0.8991979602127138 1102 4 -1.0334302420028614 1102 5 -0.8960446480477486 1102 6 -1.6864546708385504 1102 7 -1.0913211113789885 1102 8 -0.3550086870365696 1102 9 -0.5636197642979965 1102 10 -0.43606021451447674 1102 11 -0.8517928687653367 1102 12 -0.7125597850116563 1102 13 -0.7060177852014746 1102 14 -1.0281018340492452 1102 15 -0.6458582339708343 1102 16 -0.7097230867330921 1102 17 -1.1091390462578161 1102 18 -1.2644422555248411 1102 19 -0.7644413330484809 1102 20 -0.87817829292959 1102 21 -0.7990008245845885 1102 22 -0.9127122783614041 1102 23 -1.2886945863164314 1102 24 -5.059011920884547 1102 25 -3.3538205330333533 1103 1 -0.7672893550377218 1103 2 -1.018582420270878 1103 3 -0.9509867954731952 1103 4 -0.9834840633161015 1103 5 -1.249214318290928 1103 6 -0.626908621785919 1103 7 -0.5897041570865973 1103 8 -0.43286787196197096 1103 9 -0.6696419641428975 1103 10 -0.5073485694342068 1103 11 -0.8576426089183471 1103 12 -0.9379796491273069 1103 13 -0.6030246257167704 1103 14 -0.8705269182535602 1103 15 -0.5679747426459016 1103 16 -0.6458169325842178 1103 17 -0.9130489042389911 1103 18 -1.0749275733020838 1103 19 -2.389937588705507 1103 20 -1.2192966187973866 1103 21 -1.0173452115424695 1103 22 -0.8125189846127796 1103 23 -1.0274459318492646 1103 24 0.6563601406453685 1103 25 -0.16449914455921544 1104 1 0.04872136076843958 1104 2 0.23407547577149757 1104 3 -1.2675414930412572 1104 4 -0.49559530095248405 1104 5 -0.04043492568146689 1104 6 -1.8729014433033697 1104 7 -0.39572245287191876 1104 8 0.35773895324970795 1104 9 0.457263269965388 1104 10 0.5518075723679345 1104 11 0.6383499241676276 1104 12 0.7550127351280267 1104 13 0.1985460567236359 1104 14 0.31792036281829894 1104 15 0.6027363101635927 1104 16 0.3094554217203864 1104 17 0.2397320943569241 1104 18 0.3280792671387829 1104 19 -0.47477566904405677 1104 20 0.25225769227250083 1104 21 0.3934169743989244 1104 22 0.5929268323809112 1104 23 0.798014952804678 1104 24 2.2285101203974733 1104 25 1.3989782776017847 1105 1 0.10395958915080428 1105 2 0.05724657281369616 1105 3 0.5231890029877135 1105 4 0.4050790747673254 1105 5 0.415970312214057 1105 6 -0.11550028627967635 1105 7 0.16015153939239823 1105 8 0.38818239023585477 1105 9 -0.4675468330217001 1105 10 0.03823351419720211 1105 11 -0.01676314860237227 1105 12 0.1610861109343773 1105 13 0.22927908249371143 1105 14 -0.07668929147261139 1105 15 0.6856785322082928 1105 16 0.14816778349999282 1105 17 0.0357484178442391 1105 18 0.0294200299222922 1105 19 -0.667032664111062 1105 20 0.1470404341124194 1105 21 0.1888973922056323 1105 22 0.13608221839943013 1105 23 -0.11705897555038547 1105 24 -4.4419345148870875 1105 25 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1107 12 0.5324120112160884 1107 13 0.15739443055738178 1107 14 0.365539096675235 1107 15 -0.99040036335564 1107 16 -0.28550750414891723 1107 17 -0.009462948954798378 1107 18 -0.2200984339137051 1107 19 0.8892031231475386 1107 20 0.8260743071056638 1107 21 0.39846744617162955 1107 22 0.7105866816244983 1107 23 0.22442016778192908 1107 24 -3.4808456067650333 1107 25 -1.3737592779654868 1108 1 0.8234601067491489 1108 2 0.3430384982795367 1108 3 0.8595049967765362 1108 4 1.0217780913304715 1108 5 1.006912341199559 1108 6 -2.114109240731022 1108 7 -0.8913883156800644 1108 8 0.733465775329023 1108 9 0.5716288916159797 1108 10 0.7283215059156305 1108 11 0.7544907333852038 1108 12 0.8577023117625373 1108 13 0.7633370535082022 1108 14 0.648343058423869 1108 15 -0.3756633414977131 1108 16 0.6704180422991683 1108 17 0.6710356849249806 1108 18 0.6389179595289178 1108 19 0.7059551723278616 1108 20 -0.3240452284134605 1108 21 0.378351325953048 1108 22 0.5900094958321489 1108 23 0.6706804136835629 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0.5951184760392074 2215 4 0.7822379607621137 2215 5 0.7432794686957251 2215 6 1.51047313254087 2215 7 1.0429338809405202 2215 8 -0.20815768857266548 2215 9 0.2065799284823413 2215 10 -0.1640172037617873 2215 11 -0.22014924357332433 2215 12 -0.4993493395431443 2215 13 -0.2788288451093095 2215 14 -0.11105114280501027 2215 15 -0.17197190524579042 2215 16 0.050491967030353635 2215 17 -0.2160514886541236 2215 18 -1.1374069252637042 2215 19 0.12895344421726662 2215 20 -3.4271798290278994 2215 21 -2.0160515158288277 2215 22 -1.2107153505580257 2215 23 -1.112196890463899 2215 24 -1.0636202158154227 2215 25 -0.9633443425571642 2216 1 -0.08307211338255627 2216 2 -0.8873981589797921 2216 3 0.12043808708697767 2216 4 -0.07971434360210594 2216 5 -0.2708471445326417 2216 6 -1.1580738456228374 2216 7 -9.872399212512928 2216 8 -10.592969823604811 2216 9 -5.229861583454575 2216 10 -3.1000533764926494 2216 11 -2.0025206730117873 2216 12 -1.5496226725585664 2216 13 -1.3208115824278295 2216 14 -1.3183030296915277 2216 15 -2.1708352213973683 2216 16 -1.3344198771113085 2216 17 -1.5139458923719786 2216 18 -1.296300761073148 2216 19 -0.6445534118678111 2216 20 -1.5314642861117989 2216 21 -4.528090740525471 2216 22 -2.8963273260555944 2216 23 -2.650030428273162 2216 24 -3.1260164424759047 2216 25 -2.57863971995614 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37281.0 3 1.0 4 239.0 5 1559.0 6 2121.0 7 1696.0 8 1347.0 9 1233.0 10 1169.0 11 1144.0 12 1110.0 13 1110.0 14 1145.0 15 1201.0 16 1228.0 17 1398.0 18 1612.0 19 2012.0 20 2684.0 21 3314.0 22 4102.0 23 5008.0 24 5606.0 25 6711.0 26 7444.0 27 8419.0 28 9473.0 29 11067.0 30 13208.0 31 16090.0 32 20756.0 33 26930.0 34 36577.0 35 51139.0 36 73561.0 37 115393.0 38 153827.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.36163382441605 18.334550302174158 12.656157782794129 25.64765809061566 2 14.42859844782344 19.57513020895418 41.737910076750566 24.25836126647181 3 19.10337735492187 24.554764201849764 28.900551687088043 27.44130675614032 4 12.506632858944908 16.42264100584327 37.87910562053374 33.19162051467808 5 12.874899963161038 37.532075303921445 36.385938948946276 13.20708578397124 6 31.90253212594099 36.589037245926 18.83859146028737 12.669839167845637 7 28.65901615332444 30.750382897154303 22.192750905102006 18.397850044419254 8 24.816451545118216 37.82578656801003 20.40253306419261 16.95522882267914 9 26.333388906089983 15.621819452052618 19.863767356562747 38.181024285294654 10 14.921813091443825 29.025629298612532 34.40192215769506 21.65063545224858 11 34.49811057127433 22.50293734717856 24.215648915563175 18.78330316598393 12 23.549081624876152 24.659420013718467 31.488961715316417 20.302536646088967 13 29.061452088202415 22.01678564170308 25.331044863326895 23.590717406767606 14 20.754633099190876 22.251101909129016 27.230175162269614 29.764089829410494 15 23.327442845597773 31.116064836646135 22.771736455450604 22.784755862305484 16 22.075633174183036 27.903804840561254 28.069253401460458 21.95130858379525 17 21.830477149203798 28.71199406798634 27.645470091441293 21.81205869136857 18 21.746855098764904 27.57367475933944 29.35590453916857 21.32356560272709 19 24.077438040675297 26.762871406613232 26.771922358709876 22.387768194001602 20 23.557552614656686 27.534911045306952 27.72512563213007 21.18241070790629 21 22.971265079561658 26.711028125228236 26.828520893072056 23.48918590213805 22 23.185929791858854 28.224091912996396 26.755402000276273 21.834576294868477 23 22.40481668310017 27.86312084101421 27.203399114972587 22.528663360913036 24 23.629337131225757 27.03076896207347 27.53980086468738 21.80009304201339 25 22.762246200549992 27.288878868516488 27.600710035120702 22.348164895812825 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 189.0 1 189.0 2 262.5 3 336.0 4 336.0 5 336.0 6 462.5 7 589.0 8 589.0 9 589.0 10 851.5 11 1114.0 12 1114.0 13 1114.0 14 1870.0 15 2626.0 16 2626.0 17 2626.0 18 4651.0 19 6676.0 20 6676.0 21 6676.0 22 10460.5 23 14245.0 24 14245.0 25 14245.0 26 20664.5 27 27084.0 28 27084.0 29 27084.0 30 36132.5 31 45181.0 32 45181.0 33 45181.0 34 51755.0 35 58329.0 36 58329.0 37 58329.0 38 64013.0 39 69697.0 40 69697.0 41 69697.0 42 74003.5 43 78310.0 44 78310.0 45 78310.0 46 81529.5 47 84749.0 48 84749.0 49 84749.0 50 82047.0 51 79345.0 52 79345.0 53 79345.0 54 70608.0 55 61871.0 56 61871.0 57 61871.0 58 53191.5 59 44512.0 60 44512.0 61 44512.0 62 36402.5 63 28293.0 64 28293.0 65 28293.0 66 21854.0 67 15415.0 68 15415.0 69 15415.0 70 11249.5 71 7084.0 72 7084.0 73 7084.0 74 4950.0 75 2816.0 76 2816.0 77 2816.0 78 1897.0 79 978.0 80 978.0 81 978.0 82 652.5 83 327.0 84 327.0 85 327.0 86 222.0 87 117.0 88 117.0 89 117.0 90 71.0 91 25.0 92 25.0 93 25.0 94 14.0 95 3.0 96 3.0 97 3.0 98 3.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20701205718231824 2 0.11652365795385092 3 0.11969868950572696 4 0.07508949620186851 5 0.023336481906288942 6 0.020320201932006697 7 0.28718160386718844 8 0.27670399974599746 9 0.017462673535318256 10 0.03270282498432328 11 0.015081399871411221 12 0.01698641880253685 13 0.014446393561036013 14 0.015716406181786432 15 0.013652635673067 16 0.017780176690505863 17 0.017780176690505863 18 0.013652635673067 19 0.023336481906288942 20 0.015875157759380233 21 0.014128890405848407 22 0.01619266091456784 23 0.016510164069755445 24 0.014605145138629814 25 0.013811387250660804 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 629915.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.40255675630122 #Duplication Level Percentage of deduplicated Percentage of total 1 92.4393140668789 78.021144520433 2 5.637536899534362 9.516450562573828 3 0.965107417115543 2.4437260064706567 4 0.34513175754870284 1.1652001101962552 5 0.18342236484453156 0.7740658279582783 6 0.0955603230187172 0.48393213523426537 7 0.066336272768762 0.3919265719156841 8 0.03857657930773861 0.2604769539588291 9 0.0321927767190532 0.2445437397755538 >10 0.16801788655453145 2.5504178807337916 >50 0.015437311513918738 0.9166705833840417 >100 0.011672018874789384 1.9756988968546754 >500 0.0013178085825861863 0.8944192609094924 >1k 3.765167378817675E-4 0.3613269496017384 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1253 0.19891572672503435 No Hit GTCCTACAGTGTGCATTTCTCATTT 1021 0.16208536072327218 No Hit GTCCTAAAGTGTGTATTTCTCATTT 919 0.14589269980870434 No Hit CTGTAGGACGTGGAATATGGCAAGA 908 0.14414643245517253 No Hit GTATCAACGCAGAGTACTTTTTTTT 857 0.1360501019978886 No Hit GTACATGGGAAGCAGTGGTATCAAC 829 0.13160505782526213 No Hit CCCATGTACTCTGCGTTGATACCAC 735 0.11668240953144472 No Hit CTTTAGGACGTGAAATATGGCGAGG 734 0.11652365795385092 No Hit CTGTAGGACCTGGAATATGGCGAGA 647 0.10271227070319013 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.5875157759380235E-4 0.0 8 0.0 0.0 0.0 1.5875157759380235E-4 0.0 9 0.0 0.0 0.0 1.5875157759380235E-4 0.0 10 0.0 0.0 0.0 1.5875157759380235E-4 0.0 11 0.0 0.0 1.5875157759380235E-4 1.5875157759380235E-4 0.0 12 0.0 0.0 1.5875157759380235E-4 1.5875157759380235E-4 3.175031551876047E-4 13 0.0 0.0 1.5875157759380235E-4 1.5875157759380235E-4 3.175031551876047E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 55 1.1445434E-5 15.518949 18 TGTACCG 50 8.5745094E-5 15.226069 5 CCAACGA 55 1.9857756E-4 13.7946205 19 GTGTAGA 100 9.509677E-9 13.350466 1 TAGGACC 295 0.0 13.233416 4 TAGGACT 90 5.2413816E-7 12.695473 4 TAAGACT 60 4.0084304E-4 12.695471 4 CTACACT 155 1.8189894E-12 12.28594 4 AGGACCT 430 0.0 12.172003 5 CTGTAGG 815 0.0 12.051693 1 GTCCTAT 95 9.90125E-7 12.045534 1 TGTAGGA 850 0.0 11.998453 2 GTAGGAC 825 0.0 11.89896 3 GGACCTG 430 0.0 11.508076 6 CTTAGAC 75 2.0088945E-4 11.436866 3 TAGAGTG 75 2.0373744E-4 11.419553 5 GTCCTAC 735 0.0 11.41731 1 GTGCTAA 85 5.1098705E-5 11.21888 1 GTCTTAG 85 5.1098705E-5 11.21888 1 TCCTACA 785 0.0 11.170653 2 >>END_MODULE