FastQCFastQC Report
Thu 26 May 2016
SRR1926850_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926850_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629915
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13130.20844082138066244No Hit
GTCCTACAGTGGACATTTCTAAATT12250.19447068255240785No Hit
CTGTAGGACGTGGAATATGGCAAGA11270.17891302794821523No Hit
GTCCTACAGTGTGCATTTCTCATTT10440.16573664700792964No Hit
GTACATGGGAAGCAGTGGTATCAAC9670.15351277553320686No Hit
GTCCTAAAGTGTGTATTTCTCATTT9160.14541644507592294No Hit
CCCATGTACTCTGCGTTGATACCAC9060.14382892929998492No Hit
CTTTAGGACGTGAAATATGGCGAGG9000.1428764198344221No Hit
GGTATCAACGCAGAGTACTTTTTTT8540.13557384726510718No Hit
CTGTAGGACCTGGAATATGGCGAGA7790.12366747894557203No Hit
TATCAACGCAGAGTACTTTTTTTTT7080.11239611693641205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2650.015.10642054
CCCCTAC456.5396226E-414.84087851
TCCAACG400.005331862714.2267818
TTATTGT500.001460982713.3440044
GATATAC1650.013.2989691
TAGTCCC500.001517247413.2772739
GGCGAGG2300.013.19585419
TCAGTAC603.971011E-412.709593
TAACACT603.9736857E-412.7085764
TGTAGGA8050.012.6792242
ACGAAAT604.1453948E-412.64502312
AGGGTGT1001.3765566E-712.3908616
ACTGTTC2000.012.32889658
GAATAGG550.002968655412.1425381
GTATAGG550.002968655412.1425381
ATAGGAG550.002988250212.1318813
TAGGAAC550.00299003712.1309144
GTCCTAG959.844771E-712.0512411
GGTCTAC959.844771E-712.0512411
CTGTAGG7500.011.9575091
Produced by FastQC (version 0.11.5)