##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926850_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 629915 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.987299873792495 31.0 30.0 33.0 26.0 34.0 2 30.255909130596987 31.0 30.0 34.0 26.0 34.0 3 30.29565100053182 31.0 30.0 34.0 26.0 34.0 4 33.893418953350846 35.0 35.0 37.0 30.0 37.0 5 33.94371304064834 37.0 35.0 37.0 30.0 37.0 6 33.90791138486939 37.0 35.0 37.0 30.0 37.0 7 33.916869736393004 37.0 35.0 37.0 30.0 37.0 8 33.96192184659835 37.0 35.0 37.0 30.0 37.0 9 35.51519014470206 39.0 35.0 39.0 30.0 39.0 10 35.39702658295167 39.0 35.0 39.0 30.0 39.0 11 35.44806680266385 39.0 35.0 39.0 30.0 39.0 12 35.35326353555639 39.0 35.0 39.0 30.0 39.0 13 35.36226951255328 39.0 35.0 39.0 30.0 39.0 14 36.47470690489987 40.0 37.0 41.0 30.0 41.0 15 36.467110641912 40.0 37.0 41.0 30.0 41.0 16 36.44384877324718 40.0 37.0 41.0 30.0 41.0 17 36.419989998650614 40.0 37.0 41.0 30.0 41.0 18 36.39148774040942 40.0 37.0 41.0 30.0 41.0 19 36.46213695498599 40.0 37.0 41.0 30.0 41.0 20 36.42455569402221 40.0 37.0 41.0 30.0 41.0 21 36.37307731995587 40.0 37.0 41.0 30.0 41.0 22 36.29001690704301 40.0 37.0 41.0 30.0 41.0 23 36.21632601223975 40.0 37.0 41.0 30.0 41.0 24 36.140291944151194 40.0 37.0 41.0 29.0 41.0 25 36.0282180929173 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.24417358490122965 1101 2 -0.32681126281304884 1101 3 -0.13213822910739736 1101 4 -0.2156325883535004 1101 5 -0.3384387690289685 1101 6 -0.2138411673620766 1101 7 -0.23946775708111545 1101 8 -0.30554330173982436 1101 9 -0.356664415595624 1101 10 -0.39878401924613627 1101 11 -0.33673551327655105 1101 12 -0.4073646986756714 1101 13 -0.2591033530643685 1101 14 -0.1921415298941298 1101 15 -0.19667087981561338 1101 16 -0.3091326421180369 1101 17 -0.13415277142053128 1101 18 -0.36035420814307173 1101 19 -0.3035714467034438 1101 20 -0.2629716138470073 1101 21 -0.23652929970158 1101 22 -0.2615589609368385 1101 23 -0.22400224078593567 1101 24 -0.13534215865551857 1101 25 -0.3276117537321426 1102 1 -0.7910012008961367 1102 2 -0.5295414908245988 1102 3 -0.3928693209712719 1102 4 -0.5748155659160687 1102 5 -0.6733975087138901 1102 6 -0.7082511334899735 1102 7 -0.6958795873114028 1102 8 -0.5666563072639335 1102 9 -0.7322492208878515 1102 10 -0.41941417678552284 1102 11 -0.6282288411539767 1102 12 -0.5915061885936055 1102 13 -0.6157129600115638 1102 14 -0.5982566950490522 1102 15 -0.6975733781674762 1102 16 -0.6373487870633667 1102 17 -0.44950888317574567 1102 18 -0.5082502730683913 1102 19 -0.5630635924671594 1102 20 -0.5514596449456874 1102 21 -0.4650932588731962 1102 22 -0.6126990186785406 1102 23 -0.46294834094733517 1102 24 -0.43302794373815345 1102 25 -0.47724688978887997 1103 1 -0.9070735182219707 1103 2 -0.9356759630863642 1103 3 -0.7621946447672379 1103 4 -0.9770482823298323 1103 5 -0.9691369441109501 1103 6 -0.9879680218078519 1103 7 -0.8969536986810098 1103 8 -0.8945635262645908 1103 9 -0.8896296854873214 1103 10 -0.7520646160368898 1103 11 -0.8792213524680292 1103 12 -0.8436723517705644 1103 13 -0.8302146209488654 1103 14 -0.8470581580628149 1103 15 -0.8840543902396547 1103 16 -0.8797796585056616 1103 17 -0.8661078391580119 1103 18 -0.832383055456674 1103 19 -0.9388358993759596 1103 20 -0.7141284876138343 1103 21 -0.904357353691303 1103 22 -0.945663126228574 1103 23 -1.1693559708418917 1103 24 -1.0081497650976914 1103 25 -1.177364949443735 1104 1 -0.4346308382089994 1104 2 -0.32172072534383744 1104 3 -0.054916826416768316 1104 4 -0.27789961667152596 1104 5 -0.4759421963327384 1104 6 -0.5908117254064393 1104 7 -0.46287471229843646 1104 8 -0.3127683979875826 1104 9 -0.5075768148442208 1104 10 -0.472901021165427 1104 11 -0.2814805785256169 1104 12 -0.48884049001461705 1104 13 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0.06102164255839426 2211 12 -0.04300490398041035 2211 13 0.10372434769294614 2211 14 0.18825328052743373 2211 15 0.08802626658147261 2211 16 0.13353648587728628 2211 17 0.09697825483294764 2211 18 0.14502786947609536 2211 19 0.0820152288605911 2211 20 -0.014209034357939743 2211 21 -0.06721524954785707 2211 22 0.029540966096412546 2211 23 -0.1768285071540845 2211 24 -0.04456091547666574 2211 25 -0.19244655991290216 2212 1 -0.08928700423102853 2212 2 -0.1996988852456667 2212 3 -0.12152757249067392 2212 4 -0.07550124458211371 2212 5 -0.22131709730668803 2212 6 -0.329522755743362 2212 7 -0.6767740862984724 2212 8 -0.6461638653128645 2212 9 -0.7113048543614369 2212 10 -0.6528565953611789 2212 11 -0.5711616758119149 2212 12 -0.6721357662778757 2212 13 -0.6856989347661226 2212 14 -0.7216009369066185 2212 15 -0.839321858765885 2212 16 -0.607475014272346 2212 17 -0.6918807080160079 2212 18 -0.7753688643104084 2212 19 -0.6589962712890411 2212 20 -0.7441821009220035 2212 21 -0.7298119747840559 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2214 9 0.588327742810506 2214 10 0.4266886024172294 2214 11 0.4961060311515624 2214 12 0.48657502488095616 2214 13 0.5487528758112816 2214 14 0.3727271407134367 2214 15 0.13542902584793381 2214 16 0.2563283506494969 2214 17 0.4418286144353303 2214 18 0.4174656507675678 2214 19 0.29273974096675914 2214 20 0.4163516951671653 2214 21 0.42696644922502003 2214 22 0.6351851460015396 2214 23 0.6045199994773398 2214 24 0.6886333415551036 2214 25 0.6892125688550124 2215 1 0.07981293120831978 2215 2 -0.06379648314410602 2215 3 -0.045403883183254834 2215 4 0.08923973362310278 2215 5 0.07683290603265647 2215 6 0.18652613225503956 2215 7 -0.043652244761481995 2215 8 -0.020112528462092882 2215 9 -0.18662710107218317 2215 10 0.030355408467933387 2215 11 -0.317015323488846 2215 12 -0.15218207185012744 2215 13 0.025915304197347666 2215 14 -0.19072981332862327 2215 15 -0.08344127371279342 2215 16 -0.0679171434381729 2215 17 0.041888966286620644 2215 18 -0.04972492744627033 2215 19 -0.23280245575440972 2215 20 -0.12150572789499847 2215 21 -0.21086898851989133 2215 22 -0.15448089271848175 2215 23 -0.05726477719925782 2215 24 -0.08679138459837077 2215 25 -0.21503490822206572 2216 1 -0.41701501855563095 2216 2 -0.031181199905205403 2216 3 -1.708270043212039 2216 4 -1.4283736638130762 2216 5 -0.6633528997253322 2216 6 -0.3752505973728759 2216 7 -0.28937537424026516 2216 8 -0.2710474097432254 2216 9 -0.45712404260363115 2216 10 -0.13598019505396763 2216 11 -0.18995817282838345 2216 12 0.07631565868383205 2216 13 -0.2627668448374223 2216 14 -0.42783113945978357 2216 15 -0.5338533324457941 2216 16 -0.2798406188642133 2216 17 -0.219295144416499 2216 18 -0.0850384258921153 2216 19 -0.17614080344441163 2216 20 -0.2035064995568021 2216 21 -0.31537193860898327 2216 22 -0.31820585164266646 2216 23 -0.2507117211150529 2216 24 -0.45435731182806194 2216 25 -0.32062685519415624 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13956.0 3 13.0 4 200.0 5 1464.0 6 1996.0 7 1401.0 8 1106.0 9 1010.0 10 885.0 11 793.0 12 796.0 13 728.0 14 725.0 15 695.0 16 774.0 17 764.0 18 903.0 19 1100.0 20 1417.0 21 1758.0 22 2311.0 23 2792.0 24 3310.0 25 3987.0 26 4960.0 27 5193.0 28 6135.0 29 7559.0 30 9823.0 31 12491.0 32 16687.0 33 22052.0 34 29744.0 35 42204.0 36 62287.0 37 108197.0 38 257699.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.98674802007028 19.20551694323963 10.591729988922248 28.21600504776784 2 14.394524483770185 20.45992683632249 40.03550122839971 25.11004745150761 3 18.92914926081703 24.327701538700925 29.003027863649283 27.74012133683276 4 12.404848273179715 17.310907027138583 37.17580943460626 33.10843526507545 5 13.214179118694872 38.4045316280458 35.054390967225714 13.32689828603362 6 32.43864480971807 37.38146704493141 17.348621091180178 12.831267054170336 7 28.62931524510878 31.54213546479687 21.19046938561643 18.638079904477916 8 25.35559432954255 38.04506073562262 19.3534764019363 17.245868532898527 9 26.424401574315603 15.730137284257955 19.51541689356858 38.330044247857856 10 15.150620341485485 29.265353749434432 33.68561426904484 21.898411640035246 11 34.46767098016616 22.695960995930992 24.007474403976612 18.82889361992624 12 23.684026708407817 24.945786250186533 31.13520258320977 20.234984458195882 13 29.37489681098312 21.911473348658227 25.174945071692555 23.538684768666098 14 20.775660208123316 22.48128715779113 26.91236119158934 29.83069144249621 15 23.097610384002337 31.044687025712342 22.913928101960334 22.943774488324983 16 22.24021412480771 28.060859198016235 27.726844818252676 21.972081858923374 17 21.61212227046506 28.95851027519586 27.525459784256608 21.90390767008247 18 21.7353825052031 27.60063691335153 29.306269278455915 21.35771130298946 19 23.5809593357834 26.893152250700492 27.044125000992196 22.48176341252391 20 23.70521596667471 27.39954152326039 27.69021704619058 21.205025463874318 21 22.865666807447184 26.577350425993373 26.939992569329114 23.61699019723033 22 23.3964900026194 28.11633315606074 26.462617972266102 22.024558869053763 23 22.333683645704017 27.878726302320633 27.26769051013313 22.51989954184222 24 23.280601350975925 26.86886325932864 27.924084995594644 21.92645039410079 25 22.73876127999797 27.386183947316617 27.579555341051254 22.295499431634163 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 187.0 1 187.0 2 197.5 3 208.0 4 208.0 5 208.0 6 342.5 7 477.0 8 477.0 9 477.0 10 779.5 11 1082.0 12 1082.0 13 1082.0 14 1771.5 15 2461.0 16 2461.0 17 2461.0 18 4354.5 19 6248.0 20 6248.0 21 6248.0 22 9921.5 23 13595.0 24 13595.0 25 13595.0 26 20222.5 27 26850.0 28 26850.0 29 26850.0 30 35684.5 31 44519.0 32 44519.0 33 44519.0 34 51034.5 35 57550.0 36 57550.0 37 57550.0 38 62809.5 39 68069.0 40 68069.0 41 68069.0 42 72944.0 43 77819.0 44 77819.0 45 77819.0 46 81616.5 47 85414.0 48 85414.0 49 85414.0 50 82918.0 51 80422.0 52 80422.0 53 80422.0 54 71626.5 55 62831.0 56 62831.0 57 62831.0 58 54174.0 59 45517.0 60 45517.0 61 45517.0 62 37280.5 63 29044.0 64 29044.0 65 29044.0 66 22543.5 67 16043.0 68 16043.0 69 16043.0 70 11622.5 71 7202.0 72 7202.0 73 7202.0 74 5060.0 75 2918.0 76 2918.0 77 2918.0 78 1954.0 79 990.0 80 990.0 81 990.0 82 673.0 83 356.0 84 356.0 85 356.0 86 223.0 87 90.0 88 90.0 89 90.0 90 54.5 91 19.0 92 19.0 93 19.0 94 11.5 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1152536453331005 2 0.1017597612376273 3 0.015557654604192629 4 0.0 5 0.004921298905407872 6 0.3390933697403618 7 0.017938928268099664 8 0.008572585190065327 9 0.008572585190065327 10 0.003175031551876047 11 0.005080050483001674 12 1.5875157759380232E-4 13 4.7625473278140696E-4 14 0.0 15 0.0036512862846574535 16 3.1750315518760464E-4 17 0.0 18 3.1750315518760464E-4 19 0.0 20 4.7625473278140696E-4 21 0.014763896716223619 22 0.0 23 1.5875157759380232E-4 24 0.0 25 0.006191311526158291 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 629915.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.30827444450387 #Duplication Level Percentage of deduplicated Percentage of total 1 90.40375045292234 73.5057295263865 2 7.190544014107804 11.693014522087237 3 1.2675569635906185 3.0918860840900404 4 0.40712678301826394 1.3241110482942793 5 0.21350813590712026 0.8679989055235282 6 0.12212568297954368 0.5957897129053927 7 0.07220884832581079 0.4109823799897604 8 0.05239961984414328 0.34084181368602184 9 0.039117221330526586 0.28624983907039714 >10 0.19783519696225824 2.8758965128294447 >50 0.018380105345377838 1.0548604171405291 >100 0.013296130953482569 2.245319119943986 >500 0.0013687193626522052 0.9586684339192462 >1k 7.821253500869742E-4 0.7486516841336204 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1313 0.20844082138066244 No Hit GTCCTACAGTGGACATTTCTAAATT 1225 0.19447068255240785 No Hit CTGTAGGACGTGGAATATGGCAAGA 1127 0.17891302794821523 No Hit GTCCTACAGTGTGCATTTCTCATTT 1044 0.16573664700792964 No Hit GTACATGGGAAGCAGTGGTATCAAC 967 0.15351277553320686 No Hit GTCCTAAAGTGTGTATTTCTCATTT 916 0.14541644507592294 No Hit CCCATGTACTCTGCGTTGATACCAC 906 0.14382892929998492 No Hit CTTTAGGACGTGAAATATGGCGAGG 900 0.1428764198344221 No Hit GGTATCAACGCAGAGTACTTTTTTT 854 0.13557384726510718 No Hit CTGTAGGACCTGGAATATGGCGAGA 779 0.12366747894557203 No Hit TATCAACGCAGAGTACTTTTTTTTT 708 0.11239611693641205 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.5875157759380235E-4 0.0 8 0.0 0.0 0.0 1.5875157759380235E-4 0.0 9 0.0 0.0 0.0 1.5875157759380235E-4 0.0 10 0.0 0.0 0.0 1.5875157759380235E-4 0.0 11 0.0 0.0 0.0 1.5875157759380235E-4 0.0 12 0.0 0.0 0.0 1.5875157759380235E-4 4.76254732781407E-4 13 0.0 0.0 0.0 1.5875157759380235E-4 6.350063103752094E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 265 0.0 15.1064205 4 CCCCTAC 45 6.5396226E-4 14.8408785 1 TCCAACG 40 0.0053318627 14.22678 18 TTATTGT 50 0.0014609827 13.344004 4 GATATAC 165 0.0 13.298969 1 TAGTCCC 50 0.0015172474 13.277273 9 GGCGAGG 230 0.0 13.195854 19 TCAGTAC 60 3.971011E-4 12.70959 3 TAACACT 60 3.9736857E-4 12.708576 4 TGTAGGA 805 0.0 12.679224 2 ACGAAAT 60 4.1453948E-4 12.645023 12 AGGGTGT 100 1.3765566E-7 12.390861 6 ACTGTTC 200 0.0 12.3288965 8 GAATAGG 55 0.0029686554 12.142538 1 GTATAGG 55 0.0029686554 12.142538 1 ATAGGAG 55 0.0029882502 12.131881 3 TAGGAAC 55 0.002990037 12.130914 4 GTCCTAG 95 9.844771E-7 12.051241 1 GGTCTAC 95 9.844771E-7 12.051241 1 CTGTAGG 750 0.0 11.957509 1 >>END_MODULE