FastQCFastQC Report
Thu 26 May 2016
SRR1926849_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926849_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672288
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17440.2594126326812318No Hit
CTGTAGGACGTGGAATATGGCAAGA14960.2225236803274787No Hit
GTCCTACAGTGTGCATTTCTCATTT13720.20407920415060213No Hit
GTCCTAAAGTGTGTATTTCTCATTT13120.19515445761340378No Hit
CTTTAGGACGTGAAATATGGCGAGG10980.16332286163072968No Hit
GTATCAACGCAGAGTACTTTTTTTT9460.14071350373649388No Hit
CTGTAGGACCTGGAATATGGCGAGA9380.1395235375315341No Hit
GTACATGGGAAGCAGTGGTATCAAC7690.11438550145175877No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAC508.452847E-515.2518783
GGCTAGT400.00511875814.3146941
CCAACGT759.848663E-713.90807119
TAGGACC3700.013.8986724
GGACGAG500.001478223613.3234686
GACGAGG500.001478223613.3234687
TCCAACG1301.4551915E-1113.12999618
TCTAGAC801.9196814E-613.1070823
ATTGGAC603.9708155E-412.7098983
CCAACGA604.1496946E-412.643719
TCGAACT604.1496946E-412.643719
CTGTAGG9350.012.4519981
CTATACT1001.3927638E-712.3801134
GTCCTAA4900.012.2697381
CCGTTTC701.1077302E-412.1921413
GTAGGAC9450.012.1046653
GTGTAGC1353.4197E-1012.0172751
TGTAGGA10000.011.9173132
CGGTTTC802.9143088E-511.8534713
GTCCTAT1302.4228939E-911.7453911
Produced by FastQC (version 0.11.5)