##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926849_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 672288 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.195880336998428 31.0 28.0 31.0 16.0 34.0 2 28.355047241658337 31.0 28.0 33.0 16.0 34.0 3 27.844676686182112 31.0 28.0 31.0 16.0 34.0 4 31.44492985149222 35.0 32.0 37.0 17.0 37.0 5 31.797397841401303 35.0 32.0 37.0 19.0 37.0 6 31.067417535341995 35.0 32.0 37.0 12.0 37.0 7 31.127420093769338 35.0 32.0 37.0 13.0 37.0 8 31.030763601313723 35.0 32.0 37.0 11.0 37.0 9 33.05377457280213 37.0 33.0 39.0 17.0 39.0 10 33.190144699890524 37.0 33.0 39.0 17.0 39.0 11 33.36549514493788 38.0 34.0 39.0 17.0 39.0 12 33.235385727545335 38.0 33.0 39.0 17.0 39.0 13 33.341704745585226 38.0 34.0 39.0 17.0 39.0 14 34.355500916273975 39.0 34.0 41.0 17.0 41.0 15 33.96300692560331 39.0 33.0 41.0 11.0 41.0 16 34.04429649197963 39.0 33.0 41.0 16.0 41.0 17 34.261874375267745 39.0 34.0 41.0 17.0 41.0 18 34.29374613260983 39.0 34.0 41.0 17.0 41.0 19 33.689219501166164 39.0 33.0 41.0 10.0 41.0 20 33.96120858917607 39.0 34.0 41.0 10.0 41.0 21 33.950509305535725 39.0 34.0 41.0 10.0 41.0 22 33.97926632633633 39.0 34.0 41.0 8.0 41.0 23 33.9707536055976 39.0 34.0 41.0 2.0 41.0 24 32.704446606216386 39.0 32.0 41.0 2.0 41.0 25 33.254703341425106 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.25504994613882204 1101 2 -0.3185385421143856 1101 3 -1.1318106906807195 1101 4 -0.431575721758918 1101 5 -0.19944882052853785 1101 6 -2.294325438326588 1101 7 -0.7864253999006507 1101 8 -0.2841257558561523 1101 9 -0.17058725568455202 1101 10 -9.341929518423026 1101 11 -4.189055598270343 1101 12 -1.749520128780766 1101 13 -1.008268874841562 1101 14 -0.7369349460337418 1101 15 -2.0608589205920396 1101 16 -0.4441985834374478 1101 17 -0.1486713635285355 1101 18 -0.15011159878426383 1101 19 -1.2677344035398477 1101 20 -0.2643470119825153 1101 21 -0.2749944914009177 1101 22 -0.007978894226049249 1101 23 -0.19340810140212739 1101 24 -0.36513087146579437 1101 25 -0.012974767783845209 1102 1 0.06831502856238458 1102 2 -0.06563127440471916 1102 3 -0.4035107656476775 1102 4 -0.34623288580174005 1102 5 0.0928589974835532 1102 6 -0.7685292463212932 1102 7 0.05691918869007395 1102 8 0.9508384663156804 1102 9 0.681710471407122 1102 10 0.7473118036918223 1102 11 0.4361994834477301 1102 12 0.34153306507725034 1102 13 0.3666803130844727 1102 14 0.14812252523062597 1102 15 0.5504670140947852 1102 16 0.4317310987802685 1102 17 0.39105499685622647 1102 18 0.08243407080141907 1102 19 0.62098562244325 1102 20 0.5228336379626413 1102 21 0.6135416919024905 1102 22 0.5014062745721688 1102 23 0.04467736093157271 1102 24 -3.3332311024947643 1102 25 -1.7868635919873483 1103 1 -0.47532215462855376 1103 2 -0.6860566232276355 1103 3 -0.3053588612077398 1103 4 -0.7335318388207774 1103 5 -0.6723713997955265 1103 6 0.08883547595215191 1103 7 -0.12068033596827377 1103 8 0.0698459857288789 1103 9 -0.25874069338481576 1103 10 -0.07569923980604187 1103 11 -0.43790054318779426 1103 12 -0.4367733253995212 1103 13 -0.34245362262146983 1103 14 -0.22683393593273138 1103 15 0.09257572800877512 1103 16 0.15782161858275856 1103 17 -0.14513448416083463 1103 18 -0.38490667950079427 1103 19 -1.786517399664234 1103 20 -0.7328202641415658 1103 21 -0.4142022792491886 1103 22 -0.44221246864089636 1103 23 -0.4181906702934626 1103 24 1.3227997947126013 1103 25 0.5282715320727291 1104 1 -0.25886083816723726 1104 2 -0.14013587730688926 1104 3 -1.43234298819187 1104 4 -0.8792971845861217 1104 5 -0.4300359574600847 1104 6 -1.987843400726728 1104 7 -0.4027094179973574 1104 8 -0.1037914879085946 1104 9 0.024942290298170633 1104 10 0.21266794856114757 1104 11 0.008031302744051061 1104 12 -0.018569307195505758 1104 13 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0.6974834205026035 2215 2 0.2518339100117366 2215 3 0.5938659449938086 2215 4 1.039954967224169 2215 5 0.8908421308598626 2215 6 1.6234686291899578 2215 7 0.9242495833802487 2215 8 -0.16284526789260667 2215 9 0.42306182795258707 2215 10 0.17331023478482876 2215 11 0.23296313232704335 2215 12 0.12155413525351833 2215 13 0.4027581853810318 2215 14 0.6170702219249193 2215 15 0.49778283786704236 2215 16 0.6970589531689271 2215 17 0.409395309903843 2215 18 -0.5256160992799792 2215 19 0.6464165914092916 2215 20 -3.2021961939826156 2215 21 -1.4389223481553017 2215 22 -0.4137417145887454 2215 23 -0.3424644154974388 2215 24 -0.34865897907175025 2215 25 0.14921742511164382 2216 1 -0.4344740180907607 2216 2 -1.0019395908347413 2216 3 -0.031604071935710465 2216 4 -0.10962030973683312 2216 5 -0.5620091566746623 2216 6 -1.5500708284713944 2216 7 -9.641204543561447 2216 8 -11.257731513400344 2216 9 -5.67909074563142 2216 10 -3.420335255131711 2216 11 -2.2802812467753952 2216 12 -1.8539537503385688 2216 13 -1.5491411879297274 2216 14 -1.7018774747693683 2216 15 -2.878488361158766 2216 16 -1.618623870793769 2216 17 -2.071876037626531 2216 18 -1.8214705945129808 2216 19 -1.0782537284695266 2216 20 -1.887714029380163 2216 21 -4.481539444000145 2216 22 -3.2563016361783355 2216 23 -3.157401746573502 2216 24 -3.3561012850505456 2216 25 -2.8736790078088497 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40880.0 3 5.0 4 282.0 5 1691.0 6 2254.0 7 1749.0 8 1534.0 9 1306.0 10 1268.0 11 1209.0 12 1190.0 13 1185.0 14 1241.0 15 1312.0 16 1373.0 17 1454.0 18 1649.0 19 2088.0 20 2744.0 21 3508.0 22 4308.0 23 5302.0 24 6098.0 25 7088.0 26 7934.0 27 8586.0 28 9822.0 29 11602.0 30 13844.0 31 16827.0 32 21509.0 33 28220.0 34 37975.0 35 53227.0 36 76561.0 37 121839.0 38 171624.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.52040702225204 18.967236945096726 13.165902568116591 25.346453464534648 2 13.502591754051476 19.822449952335557 43.69861177311725 22.97634652049571 3 19.05622998435987 25.041930438668352 29.1320473672451 26.769792209726674 4 11.86045092368354 17.09160624630668 39.17397640145217 31.873966428557605 5 12.33754930243838 38.235631063772175 36.861485998925794 12.565333634863649 6 30.72901478858571 37.7529978873449 19.313982206088017 12.204005117981373 7 27.798615462656283 31.22549458419121 22.608092382060693 18.367797571091813 8 23.615605293662195 39.51151032878768 20.82719812146899 16.045686256081133 9 25.797957015802925 15.887430559068308 20.621167172999044 37.69344525212972 10 14.25878172573726 29.99394389397202 35.09699441558577 20.650279964704954 11 33.726983163510475 22.92630893031684 25.151189206318037 18.195518699854652 12 23.202705296185595 25.021311794906893 32.499111078711564 19.276871830195947 13 28.851735644649946 22.642898905189305 25.815157154950153 22.690208295210592 14 19.697959268687114 23.54688831991145 27.944941762464648 28.810210648936785 15 22.394183692141528 32.707656740243294 23.216881217651846 21.68127834996333 16 20.756496680061474 28.90228534957175 29.646909697374547 20.694308272992227 17 20.791074598042993 29.603852717180267 28.706135098021424 20.898937586755316 18 20.48091499709154 28.441615824403392 31.138970836550488 19.938498341954574 19 23.56743905088095 27.2167982407851 27.48237660648874 21.733386101845213 20 22.852513843471325 28.44643670089473 28.67897131329241 20.02207814234153 21 21.998786035841697 27.595369083784355 27.400777770173974 23.005067110199974 22 22.034569047342753 29.444984988737833 27.838229474442173 20.68221648947724 23 21.312324165254534 28.935611194508375 28.222229826333667 21.529834813903424 24 22.98606895895074 27.693523047809478 28.770188405279633 20.550219587960154 25 21.845003957320447 28.21509970662271 28.76614914039859 21.173747195658255 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 237.0 1 237.0 2 301.5 3 366.0 4 366.0 5 366.0 6 504.5 7 643.0 8 643.0 9 643.0 10 995.5 11 1348.0 12 1348.0 13 1348.0 14 2317.5 15 3287.0 16 3287.0 17 3287.0 18 5738.0 19 8189.0 20 8189.0 21 8189.0 22 13348.0 23 18507.0 24 18507.0 25 18507.0 26 26884.5 27 35262.0 28 35262.0 29 35262.0 30 46987.5 31 58713.0 32 58713.0 33 58713.0 34 65608.5 35 72504.0 36 72504.0 37 72504.0 38 76980.5 39 81457.0 40 81457.0 41 81457.0 42 84089.0 43 86721.0 44 86721.0 45 86721.0 46 88003.0 47 89285.0 48 89285.0 49 89285.0 50 83466.5 51 77648.0 52 77648.0 53 77648.0 54 67211.5 55 56775.0 56 56775.0 57 56775.0 58 47521.0 59 38267.0 60 38267.0 61 38267.0 62 30644.5 63 23022.0 64 23022.0 65 23022.0 66 17431.0 67 11840.0 68 11840.0 69 11840.0 70 8559.0 71 5278.0 72 5278.0 73 5278.0 74 3597.0 75 1916.0 76 1916.0 77 1916.0 78 1314.0 79 712.0 80 712.0 81 712.0 82 464.5 83 217.0 84 217.0 85 217.0 86 138.0 87 59.0 88 59.0 89 59.0 90 41.0 91 23.0 92 23.0 93 23.0 94 15.0 95 7.0 96 7.0 97 7.0 98 6.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23248964729401686 2 0.13803607977533439 3 0.1395235375315341 4 0.06946427721452711 5 0.023948069874815556 6 0.022014374791755914 7 0.301656432957304 8 0.2940703984006854 9 0.0199319339330763 10 0.035550240373173406 11 0.017403255747536768 12 0.01859322195249655 13 0.017403255747536768 14 0.018890713503736493 15 0.01621328954257699 16 0.02037817125993622 17 0.020675662811176162 18 0.016510781093816936 19 0.025286781855395308 20 0.019336950830596413 21 0.016362035318196962 22 0.01874196772811652 23 0.02037817125993622 24 0.017849493074396684 25 0.017254509971916798 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 672288.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.29269952382738 #Duplication Level Percentage of deduplicated Percentage of total 1 93.74981267581525 79.02424790297599 2 4.664347004324969 7.86340801020858 3 0.7630409335007677 1.9295634039588294 4 0.25965312734230683 0.8754745217394866 5 0.13941928073162274 0.5876013769269407 6 0.08474913524630884 0.42862400353327923 7 0.062291335244401974 0.3675493363286062 8 0.03943971507827052 0.26595840419184175 9 0.02670027163489705 0.20255741767124538 >10 0.17414966656214464 2.7523232970157405 >50 0.018728672954506125 1.0885470351422297 >100 0.015194636148197821 2.674780499501434 >500 0.0015901363391232371 0.893579230904226 >1k 8.834090772906871E-4 1.0457855599015793 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1744 0.2594126326812318 No Hit CTGTAGGACGTGGAATATGGCAAGA 1496 0.2225236803274787 No Hit GTCCTACAGTGTGCATTTCTCATTT 1372 0.20407920415060213 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1312 0.19515445761340378 No Hit CTTTAGGACGTGAAATATGGCGAGG 1098 0.16332286163072968 No Hit GTATCAACGCAGAGTACTTTTTTTT 946 0.14071350373649388 No Hit CTGTAGGACCTGGAATATGGCGAGA 938 0.1395235375315341 No Hit GTACATGGGAAGCAGTGGTATCAAC 769 0.11438550145175877 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 1.487457756199724E-4 0.0 0.0 0.0 7 0.0 1.487457756199724E-4 0.0 0.0 0.0 8 0.0 1.487457756199724E-4 0.0 0.0 0.0 9 0.0 1.487457756199724E-4 0.0 0.0 0.0 10 0.0 1.487457756199724E-4 0.0 0.0 0.0 11 0.0 1.487457756199724E-4 0.0 0.0 0.0 12 0.0 1.487457756199724E-4 0.0 0.0 1.487457756199724E-4 13 0.0 1.487457756199724E-4 0.0 0.0 1.487457756199724E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAACAC 50 8.452847E-5 15.251878 3 GGCTAGT 40 0.005118758 14.314694 1 CCAACGT 75 9.848663E-7 13.908071 19 TAGGACC 370 0.0 13.898672 4 GGACGAG 50 0.0014782236 13.323468 6 GACGAGG 50 0.0014782236 13.323468 7 TCCAACG 130 1.4551915E-11 13.129996 18 TCTAGAC 80 1.9196814E-6 13.107082 3 ATTGGAC 60 3.9708155E-4 12.709898 3 CCAACGA 60 4.1496946E-4 12.6437 19 TCGAACT 60 4.1496946E-4 12.6437 19 CTGTAGG 935 0.0 12.451998 1 CTATACT 100 1.3927638E-7 12.380113 4 GTCCTAA 490 0.0 12.269738 1 CCGTTTC 70 1.1077302E-4 12.19214 13 GTAGGAC 945 0.0 12.104665 3 GTGTAGC 135 3.4197E-10 12.017275 1 TGTAGGA 1000 0.0 11.917313 2 CGGTTTC 80 2.9143088E-5 11.85347 13 GTCCTAT 130 2.4228939E-9 11.745391 1 >>END_MODULE