FastQCFastQC Report
Thu 26 May 2016
SRR1926849_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926849_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672288
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT18370.27324598981388926No Hit
CTGTAGGACGTGGAATATGGCAAGA17540.26090009043743156No Hit
GTATCAACGCAGAGTACTTTTTTTT15390.22891974867913753No Hit
GTCCTACAGTGTGCATTTCTCATTT14190.2110702556047408No Hit
GTCCTAAAGTGTGTATTTCTCATTT13980.2079465943167214No Hit
CTTTAGGACGTGAAATATGGCGAGG13480.20050930553572277No Hit
CTGTAGGACCTGGAATATGGCGAGA10890.16198414965014993No Hit
GTACATGGGAAGCAGTGGTATCAAC8950.1331274691798753No Hit
GGTATCAACGCAGAGTACTTTTTTT8830.13134251987243561No Hit
TATCAACGCAGAGTACTTTTTTTTT8060.11988909514969776No Hit
CCCATGTACTCTGCGTTGATACCAC7360.10947689085629969No Hit
CTGAAGGACCTGGAATATGGCGAGA6810.10129587319720121No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTTT551.1375016E-515.529386512
GCCGGTT508.779313E-515.18428911
CGTTTCC653.4015538E-614.60027814
CGGTTTC551.9747944E-413.80389913
TAGGACC4500.013.7438224
GTCTAAG707.01104E-613.615131
TCCAACG1051.382432E-913.55941818
CCAACGA852.7204806E-713.39989619
CTAATAC500.001478683213.3229233
TGTAGGA10550.012.8269472
TGCCGGT604.1224627E-412.65357410
CTGTAGG10300.012.3990231
GGCGAGG4200.012.20347619
GTCCTAA4300.012.190291
CCCGTTT550.003089163512.07841212
CCGTTTC550.003089163512.07841213
GTAGGAC10900.012.0482523
AGGACCT7400.011.9579825
TGGCGAG10000.011.76957618
ATAAACC657.898059E-411.7124593
Produced by FastQC (version 0.11.5)