##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926849_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 672288 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.991884430482173 31.0 30.0 33.0 26.0 34.0 2 30.286558736731877 31.0 30.0 34.0 26.0 34.0 3 30.348734470941025 31.0 30.0 34.0 26.0 34.0 4 33.905339675853206 37.0 35.0 37.0 30.0 37.0 5 33.87815192298539 37.0 35.0 37.0 30.0 37.0 6 33.823575610452664 37.0 35.0 37.0 30.0 37.0 7 33.82613701270884 37.0 35.0 37.0 29.0 37.0 8 33.865541256128324 37.0 35.0 37.0 30.0 37.0 9 35.416452472749775 39.0 35.0 39.0 30.0 39.0 10 35.32086397496311 39.0 35.0 39.0 30.0 39.0 11 35.34668624161074 39.0 35.0 39.0 30.0 39.0 12 35.23910288447808 39.0 35.0 39.0 29.0 39.0 13 35.26731698319768 39.0 35.0 39.0 30.0 39.0 14 36.37524245561426 40.0 37.0 41.0 30.0 41.0 15 36.37460136132134 40.0 37.0 41.0 30.0 41.0 16 36.35018771716883 40.0 37.0 41.0 30.0 41.0 17 36.33309534009234 40.0 37.0 41.0 29.0 41.0 18 36.31532468227903 40.0 37.0 41.0 29.0 41.0 19 36.37314960255129 40.0 37.0 41.0 29.0 41.0 20 36.334938300252276 40.0 37.0 41.0 29.0 41.0 21 36.27618520634014 40.0 37.0 41.0 29.0 41.0 22 36.19720119948593 40.0 37.0 41.0 29.0 41.0 23 36.12736803274787 40.0 37.0 41.0 29.0 41.0 24 36.05883341663097 40.0 37.0 41.0 29.0 41.0 25 35.93982489647294 40.0 36.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.031035677616642943 1101 2 -0.13879270669265864 1101 3 0.10657474747140583 1101 4 0.0016815926368920486 1101 5 -0.20878950557929699 1101 6 -0.1601675660954811 1101 7 -0.10745715477466433 1101 8 -0.18027814925441987 1101 9 -0.23609033327417706 1101 10 -0.03913601799591504 1101 11 0.08157798273261108 1101 12 0.014290343344391943 1101 13 -0.08345971364074956 1101 14 -0.008594653662576945 1101 15 -0.2013462493455549 1101 16 -0.060327659224476804 1101 17 -0.04821342884747537 1101 18 -0.12185363952983863 1101 19 -0.29086585076936444 1101 20 -0.28562733933879514 1101 21 -0.07685986797875444 1101 22 -0.24878674054694017 1101 23 -0.1417309111957863 1101 24 -0.0844682896242901 1101 25 -0.10254125333482023 1102 1 -0.6007601181055406 1102 2 -0.44400832351549013 1102 3 -0.43576830705428904 1102 4 -0.42073271114066557 1102 5 -0.6137070929913833 1102 6 -0.521574697965832 1102 7 -0.3630372907773918 1102 8 -0.5109876390092722 1102 9 -0.5146150570505483 1102 10 -0.3705624942015646 1102 11 -0.6111095725478606 1102 12 -0.34691251888186514 1102 13 -0.26357140828213943 1102 14 -0.28476823383534366 1102 15 -0.34209722626512473 1102 16 -0.308639881290091 1102 17 -0.24168027282019722 1102 18 -0.24989802323811006 1102 19 -0.2566680483021955 1102 20 -0.36004174933657396 1102 21 -0.386574523712909 1102 22 -0.4030950937454989 1102 23 -0.6078762817307108 1102 24 -0.4493367706139395 1102 25 -0.288004812651657 1103 1 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0.6515679122391589 2208 12 0.8338083693345695 2208 13 0.6192531401903381 2208 14 0.656568680472148 2208 15 1.007943322238468 2208 16 0.9948418446564773 2208 17 0.8932898457525766 2208 18 0.8604211333530145 2208 19 0.775159123668189 2208 20 0.6544672248154626 2208 21 0.5889300960938186 2208 22 0.8452280262916858 2208 23 0.639938280335727 2208 24 0.8678692138395547 2208 25 0.8123133521055692 2209 1 0.2713086301539249 2209 2 0.24666401028725105 2209 3 0.4076609291266351 2209 4 0.5181674905140525 2209 5 0.405545895784293 2209 6 0.26865601003774486 2209 7 0.3986032819140988 2209 8 0.588076967527023 2209 9 0.421478569198932 2209 10 0.36519576505016005 2209 11 0.3321910865332214 2209 12 0.3765054988977212 2209 13 0.5301255487264953 2209 14 0.4353395655211756 2209 15 0.31479448653590225 2209 16 0.41823944781790345 2209 17 0.4270658140710637 2209 18 0.3713387265066146 2209 19 0.2782749017818418 2209 20 0.26155150066194466 2209 21 0.3848001847172853 2209 22 0.5318549665929098 2209 23 0.41635191003285144 2209 24 0.33504081549420306 2209 25 0.394256682753344 2210 1 -0.07998342205459252 2210 2 0.1080704964095851 2210 3 0.21226828845869505 2210 4 -0.025116580587017268 2210 5 0.026447893454012217 2210 6 0.06837457292301963 2210 7 -0.046407881696765685 2210 8 -0.14123261882115656 2210 9 -0.08463886867414772 2210 10 -0.29107730073691584 2210 11 -0.1782997626190621 2210 12 -0.03317233015269494 2210 13 0.07335347965751993 2210 14 -0.0653101879222433 2210 15 -0.06365652334292804 2210 16 -0.04907592925076898 2210 17 -0.1302698654918899 2210 18 -0.3583693630157896 2210 19 -0.1393315758734488 2210 20 -0.2183549201169086 2210 21 -0.35479501704680416 2210 22 -0.3136500464481742 2210 23 -0.2603837875685855 2210 24 -0.09129775942898988 2210 25 0.07992695036708852 2211 1 0.02491397598350531 2211 2 0.12573430707906752 2211 3 0.10707453541185075 2211 4 0.15667003679236302 2211 5 -0.0712142337787327 2211 6 -0.2552468502123091 2211 7 -0.16190040969267017 2211 8 -0.07243792445260766 2211 9 0.029501649193079515 2211 10 -0.054792926186273405 2211 11 -0.06271780204655641 2211 12 0.039788870137698495 2211 13 -0.05110334895810098 2211 14 -0.09087562977177299 2211 15 -0.03790344844051674 2211 16 0.08969186520160832 2211 17 -0.0038911132692263095 2211 18 -0.1480397250599026 2211 19 0.0015913683171149273 2211 20 -0.1869430427693075 2211 21 -0.03111572577367383 2211 22 0.055292650066760984 2211 23 0.07754334066370205 2211 24 -0.03985824566163387 2211 25 -0.09741258830110411 2212 1 0.4281454642059508 2212 2 0.4443271066845256 2212 3 0.5121260842247004 2212 4 0.5614280274454728 2212 5 0.5139058902202365 2212 6 0.43738601421078727 2212 7 0.4751090546224006 2212 8 0.48750665176683583 2212 9 0.4427610841523304 2212 10 0.400527521900905 2212 11 0.4743527223657438 2212 12 0.5432880834018263 2212 13 0.361604196660835 2212 14 0.5181049014510677 2212 15 0.5683763477127002 2212 16 0.6386456239202829 2212 17 0.6333531975736335 2212 18 0.5904234392622101 2212 19 0.48073699664900005 2212 20 0.5334251965862791 2212 21 0.2466724896275636 2212 22 0.4369182577321524 2212 23 0.4521705218008307 2212 24 0.45543308145609274 2212 25 0.5276549300756415 2213 1 0.27510740843190007 2213 2 0.3792081874587758 2213 3 0.3203519050633403 2213 4 0.37363052665484275 2213 5 0.3492305360574548 2213 6 0.4065700004578403 2213 7 0.2933430656020519 2213 8 0.20828122827167306 2213 9 0.31192966320340787 2213 10 0.008776048080967769 2213 11 0.23901185842750294 2213 12 0.36737358523041763 2213 13 0.31333669530257424 2213 14 0.24438065782275942 2213 15 0.307842285646025 2213 16 0.2296113307456693 2213 17 0.21450452056522806 2213 18 0.212254625048665 2213 19 0.34184112496661356 2213 20 0.304565896721698 2213 21 0.2780658677769168 2213 22 0.3158590535543695 2213 23 0.3599422358108342 2213 24 0.14048485774360842 2213 25 0.1504737982143709 2214 1 0.49508965969434016 2214 2 0.4754738844893964 2214 3 0.5556349817206083 2214 4 0.502253900014459 2214 5 0.3742806016335436 2214 6 0.25749159972904323 2214 7 0.4079669258739429 2214 8 0.41452470011321907 2214 9 0.44844572738158917 2214 10 0.2532319236199143 2214 11 0.3853682625018564 2214 12 0.44470781908967183 2214 13 0.3867591388168208 2214 14 0.4219489237082712 2214 15 0.4615620597815564 2214 16 0.20493901484955757 2214 17 0.21911055356531506 2214 18 0.18356449758760363 2214 19 0.2990595993605254 2214 20 0.32351934947942595 2214 21 0.4699776267082214 2214 22 0.384445358754121 2214 23 0.450123100158649 2214 24 0.3909242567996287 2214 25 0.40661478287663044 2215 1 0.48824128482576157 2215 2 0.20024463740335108 2215 3 0.2297496331700053 2215 4 0.5305464372468407 2215 5 0.26922064818438685 2215 6 0.47750544732404165 2215 7 0.2922399497633066 2215 8 0.3993164808566192 2215 9 0.2867426240420272 2215 10 0.2536082825471624 2215 11 0.2165980586646512 2215 12 0.43591354034874286 2215 13 0.3645705550878944 2215 14 0.5136892405738251 2215 15 0.5193523878850854 2215 16 0.33507322187864474 2215 17 0.5385216234956687 2215 18 0.43546099963683105 2215 19 0.49099737267328436 2215 20 0.648862409315214 2215 21 0.5132242089317103 2215 22 0.3184422603924517 2215 23 0.3947583638587915 2215 24 0.6030835916323625 2215 25 0.5829106568061988 2216 1 -0.16997918504266352 2216 2 0.08601196046016923 2216 3 -1.8102612076628084 2216 4 -1.3164730762522296 2216 5 -0.6202993850806351 2216 6 -0.19119944441613512 2216 7 -0.1973172096414899 2216 8 -0.11250893718371913 2216 9 -0.1247063349410169 2216 10 -0.08034951245714694 2216 11 -0.20274243258842972 2216 12 0.006332917223616619 2216 13 -0.23142130686226636 2216 14 -0.7303564691294611 2216 15 -0.44453130334423463 2216 16 -0.43069870481950545 2216 17 -0.3856965000007193 2216 18 -0.2267742909865902 2216 19 -0.2737572977951004 2216 20 -0.34939266211547704 2216 21 -0.40934328639039563 2216 22 -0.3698800836035048 2216 23 -0.5961115758483544 2216 24 -0.6575010708167781 2216 25 -0.5648810804001343 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15802.0 3 11.0 4 255.0 5 1637.0 6 2346.0 7 1612.0 8 1251.0 9 1040.0 10 978.0 11 889.0 12 840.0 13 779.0 14 784.0 15 854.0 16 866.0 17 937.0 18 1006.0 19 1198.0 20 1518.0 21 1915.0 22 2507.0 23 3050.0 24 3582.0 25 4258.0 26 5149.0 27 5717.0 28 6728.0 29 8181.0 30 10177.0 31 13446.0 32 17492.0 33 23417.0 34 31234.0 35 43779.0 36 65667.0 37 113664.0 38 277722.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.279594456540195 19.69771881803858 11.036584094571209 27.986102630850013 2 13.397707777354176 20.85919629241327 41.79761961494472 23.945476315287838 3 18.63155091590459 24.869937531334752 29.266696123193743 27.231815429566915 4 11.68621186158313 17.78196249226522 38.592983959255555 31.93884168689609 5 12.555950417034214 38.873063025941356 35.74935998226842 12.821626574756003 6 31.03749365652706 38.609361473476824 18.016507955461357 12.336636914534763 7 27.747964346872617 31.821055936013614 21.81559578839622 18.61538392871755 8 24.063980196605815 39.623301121081575 19.897412705591126 16.41530597672149 9 25.850773267957134 16.066508779968224 20.092917115433824 37.98980083664082 10 14.475841120522182 30.378366638998717 34.37058391272399 20.775208327755116 11 33.88074221504244 23.01184961726004 24.875419853687124 18.231988314010394 12 23.29302812638055 25.199208076312647 32.108608377077054 19.399155420229754 13 29.02008966422029 22.65717269138432 25.748119104071783 22.574618540323613 14 20.017016516730926 23.65965181588843 27.534479270788708 28.788852396591935 15 22.12826153894212 32.67704647111936 23.294762348888163 21.899929641050356 16 20.93350151572396 29.006999997025073 29.249902571227125 20.809595916023834 17 20.53554429054215 29.825907944214386 28.579120852967776 21.05942691227569 18 20.398995668523014 28.49552572706935 31.071653815031652 20.03382478937598 19 23.06291806921746 27.422811983572494 27.701710727710037 21.812559219500006 20 22.76114629785538 28.49903166211999 28.72691086829117 20.012911171733457 21 21.953998878311694 27.358037253738093 27.549944138732315 23.1380197292179 22 22.106150935313437 29.512649340758724 27.485988147936595 20.895211575991244 23 21.387889398426566 28.97021658904605 28.101539967305627 21.540354045221758 24 22.56756033128659 27.62015683754581 29.131711409395972 20.68057142177162 25 21.946314215799298 28.349188168004225 28.599394565969256 21.10510305022722 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 235.0 1 235.0 2 236.0 3 237.0 4 237.0 5 237.0 6 406.0 7 575.0 8 575.0 9 575.0 10 909.0 11 1243.0 12 1243.0 13 1243.0 14 2142.5 15 3042.0 16 3042.0 17 3042.0 18 5438.5 19 7835.0 20 7835.0 21 7835.0 22 12529.0 23 17223.0 24 17223.0 25 17223.0 26 25629.5 27 34036.0 28 34036.0 29 34036.0 30 45768.0 31 57500.0 32 57500.0 33 57500.0 34 64661.5 35 71823.0 36 71823.0 37 71823.0 38 76112.0 39 80401.0 40 80401.0 41 80401.0 42 83686.0 43 86971.0 44 86971.0 45 86971.0 46 88965.5 47 90960.0 48 90960.0 49 90960.0 50 85394.5 51 79829.0 52 79829.0 53 79829.0 54 68647.5 55 57466.0 56 57466.0 57 57466.0 58 48353.0 59 39240.0 60 39240.0 61 39240.0 62 31285.0 63 23330.0 64 23330.0 65 23330.0 66 17732.0 67 12134.0 68 12134.0 69 12134.0 70 8730.0 71 5326.0 72 5326.0 73 5326.0 74 3615.0 75 1904.0 76 1904.0 77 1904.0 78 1290.0 79 676.0 80 676.0 81 676.0 82 450.0 83 224.0 84 224.0 85 224.0 86 145.5 87 67.0 88 67.0 89 67.0 90 38.5 91 10.0 92 10.0 93 10.0 94 5.0 95 0.0 96 0.0 97 0.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11810414584225808 2 0.11899662049597792 3 0.016510781093816936 4 0.0 5 0.006098576800418868 6 0.3433052501308963 7 0.021568137464895998 8 0.011899662049597791 9 0.011304678947117902 10 0.004462373268599171 11 0.004462373268599171 12 1.4874577561997237E-4 13 2.9749155123994474E-4 14 0.0 15 0.00312366128801942 16 2.9749155123994474E-4 17 0.0 18 0.0 19 1.4874577561997237E-4 20 2.9749155123994474E-4 21 0.012940882478937597 22 0.0 23 1.4874577561997237E-4 24 0.0 25 0.006396068351658813 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 672288.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.51198629859849 #Duplication Level Percentage of deduplicated Percentage of total 1 92.18427487409235 75.14123350483253 2 5.880766597948891 9.587059327145312 3 0.9438455790494269 2.3080418372240894 4 0.34319796071783165 1.1189898988695535 5 0.1650351449332539 0.6726171236293303 6 0.09712817666546558 0.47502663633379705 7 0.07189830249749055 0.4102401413647557 8 0.04328818436678788 0.28228047127974554 9 0.03396542014055786 0.24917299750109642 >10 0.19402650853162012 2.9546359738701002 >50 0.022291802407697462 1.2655238937833997 >100 0.017175280839057654 2.9842154040100177 >500 0.001827157535107777 1.0044192583380285 >1k 0.0012790102745754439 1.546543531818222 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1837 0.27324598981388926 No Hit CTGTAGGACGTGGAATATGGCAAGA 1754 0.26090009043743156 No Hit GTATCAACGCAGAGTACTTTTTTTT 1539 0.22891974867913753 No Hit GTCCTACAGTGTGCATTTCTCATTT 1419 0.2110702556047408 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1398 0.2079465943167214 No Hit CTTTAGGACGTGAAATATGGCGAGG 1348 0.20050930553572277 No Hit CTGTAGGACCTGGAATATGGCGAGA 1089 0.16198414965014993 No Hit GTACATGGGAAGCAGTGGTATCAAC 895 0.1331274691798753 No Hit GGTATCAACGCAGAGTACTTTTTTT 883 0.13134251987243561 No Hit TATCAACGCAGAGTACTTTTTTTTT 806 0.11988909514969776 No Hit CCCATGTACTCTGCGTTGATACCAC 736 0.10947689085629969 No Hit CTGAAGGACCTGGAATATGGCGAGA 681 0.10129587319720121 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 1.487457756199724E-4 0.0 0.0 0.0 0.0 10 1.487457756199724E-4 0.0 0.0 0.0 0.0 11 1.487457756199724E-4 0.0 0.0 0.0 0.0 12 1.487457756199724E-4 0.0 0.0 0.0 0.0 13 1.487457756199724E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGGTTT 55 1.1375016E-5 15.5293865 12 GCCGGTT 50 8.779313E-5 15.184289 11 CGTTTCC 65 3.4015538E-6 14.600278 14 CGGTTTC 55 1.9747944E-4 13.803899 13 TAGGACC 450 0.0 13.743822 4 GTCTAAG 70 7.01104E-6 13.61513 1 TCCAACG 105 1.382432E-9 13.559418 18 CCAACGA 85 2.7204806E-7 13.399896 19 CTAATAC 50 0.0014786832 13.322923 3 TGTAGGA 1055 0.0 12.826947 2 TGCCGGT 60 4.1224627E-4 12.653574 10 CTGTAGG 1030 0.0 12.399023 1 GGCGAGG 420 0.0 12.203476 19 GTCCTAA 430 0.0 12.19029 1 CCCGTTT 55 0.0030891635 12.078412 12 CCGTTTC 55 0.0030891635 12.078412 13 GTAGGAC 1090 0.0 12.048252 3 AGGACCT 740 0.0 11.957982 5 TGGCGAG 1000 0.0 11.769576 18 ATAAACC 65 7.898059E-4 11.712459 3 >>END_MODULE