FastQCFastQC Report
Thu 26 May 2016
SRR1926848_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926848_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538980
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT8270.1534379754350811No Hit
GTCCTACAGTGGACATTTCTAAATT7920.14694422798619616No Hit
GTCCTAAAGTGTGTATTTCTCATTT6720.12467995101859068No Hit
CTGTAGGACGTGGAATATGGCAAGA6590.12226798768043341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGG1003.8198777E-1115.2493791
TCCAACG508.8230925E-515.17439318
ACCGTGG456.6960114E-414.794068
GGTCTAC653.2509015E-614.6628661
TAGGACC1650.014.4285924
AACCGTG400.00520897414.2763267
AGGATTG602.5178837E-514.2763265
GATATAC1257.2759576E-1213.7244421
CTAGGAT500.001462188113.3419643
ATAACCT500.001470429813.332024
CTAGGAC603.9771537E-412.7066313
AGGACCT2750.012.459345
GTAGGAC4750.012.2384933
TGGCGAG4200.012.19370718
GCATAGA550.00299040612.1301891
GTAGAAC2000.011.9124673
GTACTGT802.804702E-511.8969386
TCCACCT1201.0200893E-811.85499412
TGTAGGA5250.011.7990152
GTTCTAA657.793416E-411.7302921
Produced by FastQC (version 0.11.5)