##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926848_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 538980 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.04482170024862 31.0 28.0 31.0 16.0 34.0 2 28.17848714238005 31.0 28.0 31.0 16.0 34.0 3 27.659701658688633 31.0 27.0 31.0 16.0 34.0 4 31.27668559130209 35.0 32.0 37.0 17.0 37.0 5 31.623158558759137 35.0 32.0 37.0 18.0 37.0 6 30.84069724294037 35.0 31.0 37.0 11.0 37.0 7 30.918746521206725 35.0 32.0 37.0 11.0 37.0 8 30.79405543804965 35.0 32.0 37.0 11.0 37.0 9 32.834218338342794 37.0 32.0 39.0 17.0 39.0 10 32.99079928754314 37.0 33.0 39.0 17.0 39.0 11 33.17590448625181 37.0 33.0 39.0 17.0 39.0 12 33.03014397565772 37.0 33.0 39.0 17.0 39.0 13 33.135379791457936 38.0 33.0 39.0 17.0 39.0 14 34.12911981891721 39.0 34.0 40.0 17.0 41.0 15 33.694085123752274 39.0 33.0 40.0 10.0 41.0 16 33.77550372926639 39.0 33.0 40.0 11.0 41.0 17 34.002753348918326 39.0 33.0 40.0 16.0 41.0 18 34.043088797357974 39.0 34.0 41.0 11.0 41.0 19 33.40154922260566 39.0 33.0 40.0 10.0 41.0 20 33.684999443393075 39.0 33.0 40.0 10.0 41.0 21 33.68353556718245 39.0 33.0 40.0 8.0 41.0 22 33.70039333555976 39.0 34.0 41.0 2.0 41.0 23 33.68837248135367 39.0 34.0 41.0 2.0 41.0 24 32.364644328175444 38.0 32.0 40.0 2.0 41.0 25 32.9326913800141 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.6155477972805592 1101 2 -0.6581670786906102 1101 3 -1.5191207155895974 1101 4 -1.0803587239325587 1101 5 -0.5985733926669816 1101 6 -2.380502122401097 1101 7 -0.8984714934582279 1101 8 -0.4921661089904923 1101 9 -0.8094042837147022 1101 10 -9.160896590275645 1101 11 -4.362264405094216 1101 12 -2.3042539438895098 1101 13 -1.4752853853420618 1101 14 -1.1505425798094038 1101 15 -2.494770071105197 1101 16 -0.7078595970731172 1101 17 -0.45004357594877575 1101 18 -0.4192754611454035 1101 19 -1.3449904799671444 1101 20 -0.625236955869326 1101 21 -0.5901742048779255 1101 22 -0.547643931583103 1101 23 -0.3787090028365796 1101 24 -0.6396027954250485 1101 25 -0.8445836873869652 1102 1 -0.1699009219758878 1102 2 -0.3446836039904042 1102 3 -0.570295523038169 1102 4 -0.4578716419397715 1102 5 -0.24475648713904974 1102 6 -1.1964083327940003 1102 7 -0.4136806189335118 1102 8 0.382699836217661 1102 9 -0.018524884280168408 1102 10 0.10198729390101846 1102 11 0.06871443862227267 1102 12 0.06037508994104712 1102 13 -0.02942402237042785 1102 14 -0.31103297661158535 1102 15 0.3463823126327341 1102 16 0.31842489156878884 1102 17 0.22936560802314432 1102 18 -0.16189026079442215 1102 19 0.3376165201303536 1102 20 0.19468114883964915 1102 21 -0.037766093344338 1102 22 -0.05618931522354842 1102 23 -0.2327160224329532 1102 24 -3.771244601021227 1102 25 -2.2643068033078 1103 1 -0.5430987932869655 1103 2 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2216 11 -1.740430860523027 2216 12 -1.1476590980931434 2216 13 -1.1543812873276948 2216 14 -1.2291526347312427 2216 15 -2.1635322172817943 2216 16 -1.242515279371382 2216 17 -1.5277284090708676 2216 18 -1.2359928248969894 2216 19 -0.6998193773055448 2216 20 -1.934806030647529 2216 21 -4.454432760011002 2216 22 -2.7892662383004705 2216 23 -2.6858784155953472 2216 24 -3.0162018659784913 2216 25 -2.4774691964701923 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33493.0 3 1.0 4 239.0 5 1389.0 6 1940.0 7 1554.0 8 1217.0 9 1178.0 10 1057.0 11 1102.0 12 991.0 13 1007.0 14 1086.0 15 1094.0 16 1121.0 17 1249.0 18 1363.0 19 1750.0 20 2358.0 21 2934.0 22 3791.0 23 4362.0 24 5242.0 25 6062.0 26 6713.0 27 7330.0 28 8359.0 29 9993.0 30 11671.0 31 14136.0 32 18428.0 33 23774.0 34 31779.0 35 43278.0 36 62059.0 37 96370.0 38 127510.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.30072467809643 18.488029239961776 12.80122105842415 25.410025023517647 2 14.416521458055456 19.78261434945923 41.65363194911444 24.14723224337087 3 19.020540705224068 24.855795220853917 28.94220105257878 27.181463021343237 4 12.41613105647949 16.602616228338885 38.071766195478226 32.9094865197034 5 12.996108516169977 37.65312163067462 36.128817950188264 13.221951902967142 6 31.44030493326801 36.8441856322095 19.028266452886772 12.687242981635713 7 28.386597429853072 31.006606769752864 22.35436175180798 18.252434048586082 8 24.727943871504294 37.73082211278166 20.55774994837027 16.983484067343777 9 26.164774783300953 15.745220187759449 20.09200547059555 37.99799955834405 10 14.796653204252538 29.403063194155727 34.32637197838095 21.473911623210785 11 34.215268022661355 22.863436205095276 24.11415295486471 18.807142817378654 12 23.08600165155831 24.872653720181486 31.61212503595387 20.429219592306335 13 28.606500338655955 22.342385807995992 25.64117314133551 23.409940712012546 14 20.85320457388019 22.21082009924177 27.501363924837534 29.434611402040506 15 23.3434218412795 30.744093954874497 23.096806996513248 22.815677207332755 16 22.056094537518835 28.373874509163517 27.62342357054313 21.946607382774516 17 21.75990617594608 28.64732658972946 27.543001144965213 22.049766089359245 18 21.580481685807545 27.949949805065515 29.057207380232658 21.412361128894283 19 23.82639388923118 27.108371129735065 26.92464721296386 22.140587768069896 20 23.244891293859162 27.671818053607083 27.806355356625918 21.27693529590784 21 22.98755232920638 26.91224280751759 26.92783023069386 23.17237463258217 22 22.932780095307372 27.982437909175932 27.308086281379435 21.776695714137258 23 22.501688675113755 27.703921437637785 27.34651613334224 22.447873753906222 24 23.374615875655053 27.1438592063657 27.59868469886136 21.882840219117888 25 22.703554290422307 27.264899851177777 27.80804530727522 22.223500551124705 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 191.0 1 191.0 2 235.5 3 280.0 4 280.0 5 280.0 6 435.5 7 591.0 8 591.0 9 591.0 10 847.0 11 1103.0 12 1103.0 13 1103.0 14 1810.0 15 2517.0 16 2517.0 17 2517.0 18 4312.0 19 6107.0 20 6107.0 21 6107.0 22 9465.0 23 12823.0 24 12823.0 25 12823.0 26 18595.0 27 24367.0 28 24367.0 29 24367.0 30 31874.5 31 39382.0 32 39382.0 33 39382.0 34 44885.0 35 50388.0 36 50388.0 37 50388.0 38 55142.5 39 59897.0 40 59897.0 41 59897.0 42 63374.5 43 66852.0 44 66852.0 45 66852.0 46 69030.5 47 71209.0 48 71209.0 49 71209.0 50 69151.0 51 67093.0 52 67093.0 53 67093.0 54 59679.5 55 52266.0 56 52266.0 57 52266.0 58 45018.0 59 37770.0 60 37770.0 61 37770.0 62 30832.5 63 23895.0 64 23895.0 65 23895.0 66 18416.0 67 12937.0 68 12937.0 69 12937.0 70 9374.5 71 5812.0 72 5812.0 73 5812.0 74 4053.5 75 2295.0 76 2295.0 77 2295.0 78 1565.0 79 835.0 80 835.0 81 835.0 82 547.0 83 259.0 84 259.0 85 259.0 86 171.5 87 84.0 88 84.0 89 84.0 90 52.0 91 20.0 92 20.0 93 20.0 94 11.5 95 3.0 96 3.0 97 3.0 98 3.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20149170655682958 2 0.1250510223013841 3 0.1267208430739545 4 0.06345318935767562 5 0.020594456195035065 6 0.02003784927084493 7 0.2784889977364652 8 0.2781179264536718 9 0.01836802849827452 10 0.03636498571375561 11 0.016512672084307397 12 0.017625885932687668 13 0.015770529518720548 14 0.017440350291290957 15 0.015399458235927123 16 0.01929570670525808 17 0.01836802849827452 18 0.015028386953133696 19 0.023748562098779177 20 0.01781142157408438 21 0.015584993877323835 22 0.017069279008497532 23 0.01781142157408438 24 0.017069279008497532 25 0.015213922594530409 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 538980.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.6952512538053 #Duplication Level Percentage of deduplicated Percentage of total 1 94.21459407401423 82.62172499095952 2 4.2886864758467755 7.521948760963595 3 0.7490944638465481 1.9707608165957284 4 0.2680060494485923 0.9401143137573627 5 0.13564501854089425 0.59477119911354 6 0.07775678231439313 0.40913403370488893 7 0.05348809134634794 0.328345612679313 8 0.03189435691439351 0.22375869145490251 9 0.025745471841678722 0.2031980059623406 >10 0.13284070977505746 2.094606888414256 >50 0.010170758378180341 0.6310381639918243 >100 0.010594515555212888 1.6194016102394995 >500 0.001483232177712397 0.8411969121632406 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 827 0.1534379754350811 No Hit GTCCTACAGTGGACATTTCTAAATT 792 0.14694422798619616 No Hit GTCCTAAAGTGTGTATTTCTCATTT 672 0.12467995101859068 No Hit CTGTAGGACGTGGAATATGGCAAGA 659 0.12226798768043341 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 1.8553564139671232E-4 0.0 0.0 0.0 0.0 12 1.8553564139671232E-4 0.0 0.0 0.0 0.0 13 1.8553564139671232E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTAGG 100 3.8198777E-11 15.249379 1 TCCAACG 50 8.8230925E-5 15.174393 18 ACCGTGG 45 6.6960114E-4 14.79406 8 GGTCTAC 65 3.2509015E-6 14.662866 1 TAGGACC 165 0.0 14.428592 4 AACCGTG 40 0.005208974 14.276326 7 AGGATTG 60 2.5178837E-5 14.276326 5 GATATAC 125 7.2759576E-12 13.724442 1 CTAGGAT 50 0.0014621881 13.341964 3 ATAACCT 50 0.0014704298 13.33202 4 CTAGGAC 60 3.9771537E-4 12.706631 3 AGGACCT 275 0.0 12.45934 5 GTAGGAC 475 0.0 12.238493 3 TGGCGAG 420 0.0 12.193707 18 GCATAGA 55 0.002990406 12.130189 1 GTAGAAC 200 0.0 11.912467 3 GTACTGT 80 2.804702E-5 11.896938 6 TCCACCT 120 1.0200893E-8 11.854994 12 TGTAGGA 525 0.0 11.799015 2 GTTCTAA 65 7.793416E-4 11.730292 1 >>END_MODULE