FastQCFastQC Report
Thu 26 May 2016
SRR1926848_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926848_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538980
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14000.2597498979553972No Hit
GGTATCAACGCAGAGTACTTTTTTT9560.17737207317525697No Hit
GTCCTACAGTGGACATTTCTAAATT8640.16030279416675944No Hit
CTGTAGGACGTGGAATATGGCAAGA8350.15492226056625477No Hit
TATCAACGCAGAGTACTTTTTTTTT7390.1371108389921704No Hit
GTCCTAAAGTGTGTATTTCTCATTT6760.12542209358417752No Hit
CTTTAGGACGTGAAATATGGCGAGG6470.12004155998367287No Hit
GTCCTACAGTGTGCATTTCTCATTT5460.10130246020260492No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGAC456.655176E-414.8063
CCTAACA604.0033265E-412.6967632
TAGGACA1600.012.4902394
CTATTAG550.003012072812.1185091
GTCTTAG550.003012072812.1185091
ATAGGTG550.003024698712.1117465
GTCCTAG802.7922633E-511.9021091
CTAGGAC802.8029259E-511.8976793
CTAGACA657.893597E-411.7124584
TAGGACC2150.011.508174
AGGACCT3150.011.4800675
GTACTAC752.0256446E-411.4260241
TCCAACG752.0859929E-411.38997918
CTGTAGG5300.011.3182331
TAAAGGC600.00579246911.1024344
GTCCTAC4000.010.949941
GTATCAA6900.010.9016411
GTACTAT700.001460601110.88192751
CTAACAG700.001465145810.8778783
GGACATG2050.010.6769056
Produced by FastQC (version 0.11.5)