FastQCFastQC Report
Thu 26 May 2016
SRR1926847_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926847_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1240
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTT40.3225806451612903No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA30.24193548387096775No Hit
AACATGTGTATTGTAGCCAATGCAA20.16129032258064516No Hit
ATGTAAAGGTTCCTCTGGTGAACTT20.16129032258064516No Hit
GCCCAGGAGGGTAGAGGTCTGAGAT20.16129032258064516No Hit
GGATACTTTTAAAAACTGCATTTCT20.16129032258064516No Hit
AGCTGATATCATGTGAAAAATCTCA20.16129032258064516No Hit
CCTCTACCATCATCATGAGATATGC20.16129032258064516No Hit
GGGCTGGTGGCATCCGAGTGCTTGG20.16129032258064516No Hit
CTTGTATTCTGTTGCTGATGCTTGC20.16129032258064516No Hit
CTCTCAAGGAGGGGGAAACCCTTTC20.16129032258064516No Hit
GTATCAACGCAGAGTACTTTTTTTT20.16129032258064516No Hit
GAAGAAGTGAGTGAAGAATTCTCTA20.16129032258064516No Hit
ATGTGAAGGATAATGTGTGTCTAGG20.16129032258064516No Hit
GTACTGGATAGCTCATATTGTTGTT20.16129032258064516No Hit
GGTATCAACGCAGAGTACTTTTTTT20.16129032258064516No Hit
ATCTCTAAAGCTGTACTTCTTTGTA20.16129032258064516No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20.16129032258064516No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)