FastQCFastQC Report
Thu 26 May 2016
SRR1926846_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926846_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264106
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT5680.21506516322991526No Hit
GTCCTACAGTGGACATTTCTAAATT4220.15978432901940887No Hit
GGTATCAACGCAGAGTACTTTTTTT3580.13555163457096772No Hit
GTCCTACAGTGTGCATTTCTCATTT3390.12835755340658675No Hit
CTGTAGGACGTGGAATATGGCAAGA3040.11510529863009548No Hit
GTCCTAAAGTGTGTATTTCTCATTT2860.10828985331647141No Hit
CTTTAGGACGTGAAATATGGCGAGG2740.10374622310738871No Hit
TATCAACGCAGAGTACTTTTTTTTT2700.10223167970436113No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATTC250.005866790719.0844463
TAGGACC1200.015.0797734
GGCGAGG704.5072375E-714.89554119
GGCGAGA1301.8189894E-1213.85389519
TGGCGAG2000.013.74452218
TAGAACA1051.2987584E-913.6058124
AGACAGT500.001467663313.3311586
CTAAACA603.985958E-412.6987564
TGTAGGA2850.012.3881482
GTCCTAC2950.012.2916771
TTATACT550.002999410712.1215414
GACCTGG1603.6379788E-1211.902827
CTACAGT4350.011.8229814
AGAACAG657.829352E-411.71975
TATGGCG2350.011.69746616
GTAGGAC2950.011.6447473
ATGGCGA2450.011.60691617
CTGTAGG3050.011.5758111
CCTACAG3400.011.5067983
TCCTACA3250.011.4506672
Produced by FastQC (version 0.11.5)