FastQCFastQC Report
Thu 26 May 2016
SRR1926846_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926846_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264106
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT9120.34531589589028644No Hit
GGTATCAACGCAGAGTACTTTTTTT6780.2567151068131735No Hit
TATCAACGCAGAGTACTTTTTTTTT4540.17190067624362945No Hit
CTGTAGGACGTGGAATATGGCAAGA4310.1631920516762209No Hit
GTCCTACAGTGGACATTTCTAAATT4290.16243477997470712No Hit
GTCCTACAGTGTGCATTTCTCATTT3540.13403709116794016No Hit
CTGTAGGACCTGGAATATGGCGAGA3180.120406200540692No Hit
ACGCAGAGTACTTTTTTTTTTTTTT3030.1147266627793386No Hit
GTCCTAAAGTGTGTATTTCTCATTT2970.11245484767479724No Hit
CTTTAGGACGTGAAATATGGCGAGG2880.1090471250179852No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG2780.10526076651041628No Hit
CACACACACACACACACACACACAC2760.10450349480890249No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATATA350.00211803216.337872
TTAGGCT350.00214237816.3099584
TCCAACG508.7429304E-515.18515518
TAGGACC1204.9112714E-1113.4783684
GGTATCA2400.012.70723151
TAGGACA905.2524956E-712.6855234
GGCGAGG1301.9645086E-1012.41094419
GACGTGA1155.300535E-912.3815467
AGGACGT2000.012.3683855
GGACGTG2000.012.3683856
ATGGCAA2000.012.33793817
GGCAAGA1850.012.31228819
CTAGGAC550.003018662312.1112073
CTGGACA550.003022960612.1089094
GTAGGAC2900.011.8129013
TTAGGAC1302.5011104E-911.7119373
GACATGG907.4601903E-611.6019687
TGTAGGA3100.011.3750222
TATGGCA2100.011.29847716
TTCCAAC855.336795E-511.16555517