##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926846_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 264106 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.129061816088996 31.0 30.0 33.0 26.0 34.0 2 30.420172203584926 31.0 30.0 34.0 27.0 34.0 3 30.4280629747147 31.0 30.0 34.0 26.0 34.0 4 33.989682173066875 37.0 35.0 37.0 30.0 37.0 5 33.969107100936746 37.0 35.0 37.0 30.0 37.0 6 33.956854444806254 37.0 35.0 37.0 30.0 37.0 7 33.96019022665142 37.0 35.0 37.0 30.0 37.0 8 33.99346474521594 37.0 35.0 37.0 30.0 37.0 9 35.5603810591202 39.0 35.0 39.0 30.0 39.0 10 35.44460936139277 39.0 35.0 39.0 30.0 39.0 11 35.48147713418097 39.0 35.0 39.0 30.0 39.0 12 35.38868484623598 39.0 35.0 39.0 30.0 39.0 13 35.419471727261026 39.0 35.0 39.0 30.0 39.0 14 36.51208605635616 40.0 37.0 41.0 30.0 41.0 15 36.5163608551112 40.0 37.0 41.0 30.0 41.0 16 36.50006058173612 40.0 37.0 41.0 30.0 41.0 17 36.4976978940274 40.0 37.0 41.0 30.0 41.0 18 36.46381377174316 40.0 37.0 41.0 30.0 41.0 19 36.54294866455135 40.0 37.0 41.0 30.0 41.0 20 36.51341885455082 40.0 37.0 41.0 30.0 41.0 21 36.4836391448888 40.0 37.0 41.0 30.0 41.0 22 36.40462541555284 40.0 37.0 41.0 30.0 41.0 23 36.33793628315903 40.0 37.0 41.0 30.0 41.0 24 36.281042460224306 40.0 37.0 41.0 30.0 41.0 25 36.17571732561926 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.09049646014439716 1101 2 -0.29555400179274827 1101 3 0.02465788991775497 1101 4 0.10379258321911067 1101 5 -0.0871439051821028 1101 6 -0.041412449686013986 1101 7 -0.26208900985220396 1101 8 -0.17939923544804515 1101 9 -0.06013293228004102 1101 10 0.2743528558414141 1101 11 -0.11012617460694685 1101 12 -0.2513607128142681 1101 13 -0.05371879964859971 1101 14 0.057359877166405226 1101 15 0.20228415828667323 1101 16 -0.020403897217150302 1101 17 -0.07609570164961355 1101 18 -0.14729517171996775 1101 19 -0.27813086344843896 1101 20 -0.21379950398079473 1101 21 0.01743588403429186 1101 22 -0.5663430301680421 1101 23 -0.21937276230250546 1101 24 -0.05851561076570988 1101 25 -0.17741441803893565 1102 1 -0.4190655815914255 1102 2 -0.6454611400356427 1102 3 -0.4647437573690638 1102 4 -0.37783693874471425 1102 5 -0.3112435498849422 1102 6 -0.42870653883330334 1102 7 -0.4201558703173234 1102 8 -0.23770026903977737 1102 9 -0.37933512866246843 1102 10 -0.2612979839518701 1102 11 -0.26298470173873056 1102 12 -0.3302932063543196 1102 13 -0.21196734616022894 1102 14 -0.3840564665028481 1102 15 -0.5995367396461404 1102 16 -0.5025381026435127 1102 17 -0.4876549716754539 1102 18 -0.40837802055717276 1102 19 -0.285640553370925 1102 20 -0.4204976628955208 1102 21 -0.5032359505910335 1102 22 -0.22937597590448178 1102 23 -0.5099493126900967 1102 24 -0.28013221283289624 1102 25 -0.5341246893567657 1103 1 -0.6963432343379452 1103 2 -0.34217698566371624 1103 3 -0.18879977137256532 1103 4 -0.4645877931249771 1103 5 -0.45029914711758323 1103 6 -0.5668660787182702 1103 7 -0.4403484184543558 1103 8 -0.5255013859856774 1103 9 -0.481839921527353 1103 10 -0.5450355850188018 1103 11 -0.5950052068650109 1103 12 -1.008921196685236 1103 13 -0.5538028050249437 1103 14 -0.8235204991776826 1103 15 -0.6922890944014952 1103 16 -0.8124905907655418 1103 17 -1.0973490618646622 1103 18 -0.7597783706446961 1103 19 -0.5587088204376869 1103 20 -0.4853888857012194 1103 21 -0.6331017503743084 1103 22 -0.5061951807056815 1103 23 -0.6903069020874497 1103 24 -0.6228637290452852 1103 25 -0.5505662997593674 1104 1 0.009482062192372354 1104 2 0.09735299992546587 1104 3 -0.011075572281555424 1104 4 -0.16043636867092914 1104 5 -0.36455477287969273 1104 6 -0.28585510432518646 1104 7 -0.06852156998966308 1104 8 -0.2114009536610979 1104 9 -0.29993104224567446 1104 10 -0.16395255652972196 1104 11 -0.36152222271690704 1104 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0.12261631046663268 2214 9 0.13877794986023417 2214 10 0.12911831137287066 2214 11 0.12050921946391924 2214 12 -0.043196848027569956 2214 13 0.06801045740129297 2214 14 0.2614339010275799 2214 15 0.26919598945081447 2214 16 0.33164441261654076 2214 17 5.097303822054755E-4 2214 18 0.0464808134571868 2214 19 -0.016222873571365426 2214 20 0.19299844432000413 2214 21 0.22459423544427182 2214 22 0.059312248212286534 2214 23 0.10463980096575654 2214 24 0.1418075092632094 2214 25 0.1995853108113863 2215 1 0.6335396095073449 2215 2 0.39798166807221236 2215 3 0.49028177939892004 2215 4 0.6857377680094459 2215 5 0.5946202650382233 2215 6 0.447543440505882 2215 7 0.6067031898635022 2215 8 0.5250694923415793 2215 9 0.31857620062685754 2215 10 0.37439949763246005 2215 11 0.12492624188203649 2215 12 0.572744209005208 2215 13 0.3083381033364745 2215 14 0.6100317854819721 2215 15 0.5022852688055082 2215 16 0.293167753458043 2215 17 0.661472039999289 2215 18 0.40187304967307114 2215 19 0.33876234906044544 2215 20 0.2742346555785531 2215 21 0.31274505247778706 2215 22 0.35372182413188114 2215 23 -0.012205473745289908 2215 24 0.2747621966259004 2215 25 0.3016300915559498 2216 1 -0.7283258959276182 2216 2 -0.27099131619512207 2216 3 -2.506075916689518 2216 4 -1.6856585118068779 2216 5 -0.7576907221828435 2216 6 -0.3497887024699793 2216 7 -0.2087644924060328 2216 8 -0.49236322950891775 2216 9 -0.3946030510625178 2216 10 -0.5692231463857595 2216 11 -0.4866341553047917 2216 12 -0.40469915008226565 2216 13 -0.41432918383568307 2216 14 -0.9640418488915046 2216 15 -0.8688403313198592 2216 16 -0.7450408218645137 2216 17 -0.8391049351314237 2216 18 -1.037079182484625 2216 19 -0.6937999428842261 2216 20 -0.9008761094002438 2216 21 -0.7654148954757289 2216 22 -0.7271401719646278 2216 23 -0.8960269011295239 2216 24 -0.8326549017827887 2216 25 -0.547159685299512 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5869.0 3 11.0 4 83.0 5 550.0 6 776.0 7 647.0 8 489.0 9 418.0 10 378.0 11 302.0 12 290.0 13 278.0 14 296.0 15 279.0 16 286.0 17 323.0 18 339.0 19 418.0 20 500.0 21 740.0 22 925.0 23 1099.0 24 1356.0 25 1598.0 26 1921.0 27 2147.0 28 2512.0 29 3070.0 30 3911.0 31 5026.0 32 6807.0 33 8988.0 34 12400.0 35 17570.0 36 26148.0 37 45781.0 38 109575.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.212430440781795 20.941304908189412 12.331124622826037 26.515140028202755 2 13.197705933414452 21.183726228246737 42.26093680703231 23.357631031306504 3 18.420095889477153 25.45009732857673 30.071879236822774 26.057927545123345 4 11.602538374743474 19.0332669458475 39.58221320227484 29.781981477134178 5 12.339494181841589 39.010250409902795 35.8309950887397 12.819260319515918 6 29.21323981976095 39.493780536917846 18.811501257570114 12.481478385751085 7 26.521213865608278 32.883669318952165 22.197395180439994 18.397721634999563 8 23.68181887032521 39.53028673563671 20.201760099365334 16.586134294672746 9 24.83555683466314 17.331192511161685 21.169903776607228 36.663346877567946 10 14.392872288864655 31.27234036108082 33.96984429904278 20.364943051011753 11 32.797406954421014 24.26625670696843 24.63431165772404 18.302024680886515 12 22.348393057280465 26.29683760942659 32.131281616332956 19.223487716959987 13 28.047390601394152 23.22843738995771 26.234461554772192 22.48971045387595 14 19.825751781481678 24.28456755999485 28.262137172196013 27.62754348632746 15 21.984013691731572 32.167239048697276 24.058220150624194 21.790527108946954 16 20.49177224296305 30.560835422141107 28.653268006027883 20.294124328867955 17 20.347890619675432 30.42528378757014 28.398446078468492 20.82837951428593 18 19.860358040771818 29.580771211340988 30.611425801956806 19.94744494593039 19 21.969209332616447 28.51090092614329 28.82441141057 20.695478330670262 20 21.89524541561436 28.920913431502104 29.602844344820014 19.58099680806352 21 21.530707301741177 28.19020426092686 28.57267281140892 21.706415625923036 22 21.13810364020507 29.61500306695039 28.824032774719242 20.4228605181253 23 21.01407021476388 29.168812286069123 28.711416714627568 21.105700784539426 24 21.233898510446565 28.469629618410792 29.94403762125813 20.352434249884517 25 21.226693727943292 28.81420111173379 29.220877572966998 20.73822758735592 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 124.0 1 124.0 2 121.5 3 119.0 4 119.0 5 119.0 6 210.5 7 302.0 8 302.0 9 302.0 10 517.5 11 733.0 12 733.0 13 733.0 14 1266.5 15 1800.0 16 1800.0 17 1800.0 18 2952.0 19 4104.0 20 4104.0 21 4104.0 22 6381.5 23 8659.0 24 8659.0 25 8659.0 26 12061.5 27 15464.0 28 15464.0 29 15464.0 30 19744.5 31 24025.0 32 24025.0 33 24025.0 34 26820.0 35 29615.0 36 29615.0 37 29615.0 38 31318.0 39 33021.0 40 33021.0 41 33021.0 42 33619.0 43 34217.0 44 34217.0 45 34217.0 46 34423.0 47 34629.0 48 34629.0 49 34629.0 50 32320.5 51 30012.0 52 30012.0 53 30012.0 54 25379.0 55 20746.0 56 20746.0 57 20746.0 58 16930.5 59 13115.0 60 13115.0 61 13115.0 62 10240.0 63 7365.0 64 7365.0 65 7365.0 66 5544.0 67 3723.0 68 3723.0 69 3723.0 70 2621.0 71 1519.0 72 1519.0 73 1519.0 74 1041.0 75 563.0 76 563.0 77 563.0 78 366.5 79 170.0 80 170.0 81 170.0 82 113.5 83 57.0 84 57.0 85 57.0 86 39.0 87 21.0 88 21.0 89 21.0 90 11.5 91 2.0 92 2.0 93 2.0 94 1.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1143480269285817 2 0.11169757597328345 3 0.01968906423935844 4 0.0 5 0.007194081164380968 6 0.3407722656812038 7 0.021582243493142902 8 0.011359075522706793 9 0.01249498307497747 10 0.0037863585075689305 11 0.005679537761353396 12 7.572717015137861E-4 13 0.0011359075522706793 14 0.0 15 0.0018931792537844653 16 0.0 17 0.0 18 7.572717015137861E-4 19 0.0 20 0.0011359075522706793 21 0.012116347224220577 22 0.0 23 7.572717015137861E-4 24 0.0 25 0.005300901910596503 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 264106.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.26913899824686 #Duplication Level Percentage of deduplicated Percentage of total 1 95.13564739332703 83.97541683449785 2 3.869464468704089 6.831085940736373 3 0.4647868344928376 1.2307900109521033 4 0.15367008149958994 0.542573031350369 5 0.08390067748796974 0.3702920281616341 6 0.051002062411912956 0.27011448817406436 7 0.03342479218994674 0.20652643394613474 8 0.026129031384025978 0.1845109682500915 9 0.01602101283762227 0.12727449081511 >10 0.13588479365930797 2.3029381288268516 >50 0.018900421260702942 1.2322847072740397 >100 0.010309320687655797 2.1233213417644468 >500 8.591100573046498E-4 0.6028715952509769 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 912 0.34531589589028644 No Hit GGTATCAACGCAGAGTACTTTTTTT 678 0.2567151068131735 No Hit TATCAACGCAGAGTACTTTTTTTTT 454 0.17190067624362945 No Hit CTGTAGGACGTGGAATATGGCAAGA 431 0.1631920516762209 No Hit GTCCTACAGTGGACATTTCTAAATT 429 0.16243477997470712 No Hit GTCCTACAGTGTGCATTTCTCATTT 354 0.13403709116794016 No Hit CTGTAGGACCTGGAATATGGCGAGA 318 0.120406200540692 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 303 0.1147266627793386 No Hit GTCCTAAAGTGTGTATTTCTCATTT 297 0.11245484767479724 No Hit CTTTAGGACGTGAAATATGGCGAGG 288 0.1090471250179852 No Hit GTGTGTGTGTGTGTGTGTGTGTGTG 278 0.10526076651041628 No Hit CACACACACACACACACACACACAC 276 0.10450349480890249 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCATATA 35 0.002118032 16.33787 2 TTAGGCT 35 0.002142378 16.309958 4 TCCAACG 50 8.7429304E-5 15.185155 18 TAGGACC 120 4.9112714E-11 13.478368 4 GGTATCA 240 0.0 12.7072315 1 TAGGACA 90 5.2524956E-7 12.685523 4 GGCGAGG 130 1.9645086E-10 12.410944 19 GACGTGA 115 5.300535E-9 12.381546 7 AGGACGT 200 0.0 12.368385 5 GGACGTG 200 0.0 12.368385 6 ATGGCAA 200 0.0 12.337938 17 GGCAAGA 185 0.0 12.312288 19 CTAGGAC 55 0.0030186623 12.111207 3 CTGGACA 55 0.0030229606 12.108909 4 GTAGGAC 290 0.0 11.812901 3 TTAGGAC 130 2.5011104E-9 11.711937 3 GACATGG 90 7.4601903E-6 11.601968 7 TGTAGGA 310 0.0 11.375022 2 TATGGCA 210 0.0 11.298477 16 TTCCAAC 85 5.336795E-5 11.165555 17 >>END_MODULE