FastQCFastQC Report
Thu 26 May 2016
SRR1926845_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926845_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences593630
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT10250.17266647575088861No Hit
GTCCTAAAGTGTGTATTTCTCATTT8010.13493253373313344No Hit
GTATCAACGCAGAGTACTTTTTTTT7920.1334164378484915No Hit
CTGTAGGACGTGGAATATGGCAAGA7920.1334164378484915No Hit
GTCCTACAGTGTGCATTTCTCATTT7270.12246685645941074No Hit
CTTTAGGACGTGAAATATGGCGAGG6630.11168573016862357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2100.014.0559714
TCCTATA954.611138E-914.0558932
GAATAGT604.0272437E-412.6882016
AATGTCC905.330585E-712.6774818
TGGCGAA604.1495363E-412.64329818
AGGACCT3100.012.27994255
GTAGGAC5200.011.9213983
GACGTGG2250.011.8423217
AGGACGT4300.011.7302025
CTAGAGT1302.5065674E-911.7191234
TGAATAG657.891586E-411.7131775
GTCCTAG1104.6428613E-711.2826141
GTCCTAC5000.011.2652571
GGACGTG4650.011.2556626
CTTAAAC600.00570043611.1266383
TACTGGG600.005794634611.1021767
GGCGAAA600.00594961511.06288619
CATTTCT6700.011.03929814
TCCTACA5500.010.92526152
TGTAGGA5850.010.92377952
Produced by FastQC (version 0.11.5)