##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1926845_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	593630
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.012357866010817	31.0	28.0	31.0	16.0	34.0
2	28.13519869278844	31.0	28.0	31.0	16.0	34.0
3	27.718952883109008	31.0	28.0	31.0	16.0	34.0
4	31.3411451577582	35.0	32.0	37.0	17.0	37.0
5	31.49793979414787	35.0	32.0	37.0	17.0	37.0
6	30.65769418661456	35.0	30.0	37.0	11.0	37.0
7	30.709425062749524	35.0	31.0	37.0	11.0	37.0
8	30.625337331334332	35.0	31.0	37.0	10.0	37.0
9	32.758652696123846	37.0	32.0	39.0	17.0	39.0
10	33.017194211882824	37.0	33.0	39.0	17.0	39.0
11	33.171226184660476	37.0	33.0	39.0	17.0	39.0
12	33.06548523491063	37.0	33.0	39.0	17.0	39.0
13	33.18260701110119	38.0	33.0	39.0	17.0	39.0
14	34.19593517847817	39.0	34.0	40.0	17.0	41.0
15	33.768635345248725	39.0	33.0	40.0	11.0	41.0
16	33.851729191583985	39.0	33.0	40.0	11.0	41.0
17	34.08169398446844	39.0	34.0	40.0	17.0	41.0
18	34.13410878830248	39.0	34.0	41.0	16.0	41.0
19	33.50472179640517	39.0	33.0	40.0	10.0	41.0
20	33.76651112645924	39.0	33.0	40.0	10.0	41.0
21	33.74904064821522	39.0	34.0	40.0	8.0	41.0
22	33.7722116469855	39.0	34.0	40.0	2.0	41.0
23	33.75971901689605	39.0	34.0	41.0	2.0	41.0
24	32.448285969374865	38.0	32.0	40.0	2.0	41.0
25	33.01530751478194	39.0	33.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.618474137197822
1101	2	-0.7580147862950888
1101	3	-1.5793467725509807
1101	4	-1.175895388087966
1101	5	-0.8550249711667703
1101	6	-3.342024408749907
1101	7	-1.6397874361222442
1101	8	-0.8971134020321614
1101	9	-1.099298518449075
1101	10	-9.560468798676695
1101	11	-4.856170505773537
1101	12	-2.129360244654656
1101	13	-1.3177434987329768
1101	14	-1.376329863981546
1101	15	-2.6940631551918344
1101	16	-1.14754913557217
1101	17	-0.9338446369035012
1101	18	-0.9329214873377438
1101	19	-1.2808011383430014
1101	20	-0.6401416411557292
1101	21	-0.8477609245508617
1101	22	-0.4877742737125459
1101	23	-0.6723551401300085
1101	24	-0.9058099318063704
1101	25	-0.7499449684342139
1102	1	0.03777491009788392
1102	2	-0.09628509102189042
1102	3	-0.3850505265523374
1102	4	-0.19330874396285935
1102	5	-0.1646032869241516
1102	6	-0.9603454319889622
1102	7	-0.22394136542119014
1102	8	0.18304540511277523
1102	9	0.2900838520177871
1102	10	0.12238945278181035
1102	11	0.14129218286191048
1102	12	0.2902342843976271
1102	13	0.22973512604703217
1102	14	0.08656386272050298
1102	15	0.7019514229160606
1102	16	0.47065964177910047
1102	17	0.16093107918369043
1102	18	0.08648873673831048
1102	19	0.2835989004580455
1102	20	0.26907222190428115
1102	21	0.3408024666607048
1102	22	-0.18529793542284523
1102	23	-0.2808872133531324
1102	24	-3.3960057385217226
1102	25	-2.252688756802211
1103	1	-0.09713633250566645
1103	2	-0.4678827241579846
1103	3	-0.24067182832748557
1103	4	-0.5814744244362338
1103	5	-0.8036565413620202
1103	6	-0.12129217755109423
1103	7	-0.4665449157170478
1103	8	-0.11044571914758095
1103	9	-0.20222384028991058
1103	10	-0.33204841704067434
1103	11	-0.51314568696057
1103	12	-0.5819550647147977
1103	13	-0.6223358798701284
1103	14	-0.7702408710073101
1103	15	-0.2258592279715117
1103	16	-0.2749024883984106
1103	17	-0.4165837137157169
1103	18	-0.8010852277587333
1103	19	-1.9317857149265727
1103	20	-0.8315194940720474
1103	21	-0.9290200185463959
1103	22	-0.6705050360145606
1103	23	-0.7566268583235427
1103	24	0.6465978117741358
1103	25	0.0751218940853633
1104	1	-0.1317135418967723
1104	2	-0.34547795770855316
1104	3	-1.431692470216614
1104	4	-0.3256649733414214
1104	5	-0.2621791601824057
1104	6	-1.421423064034336
1104	7	-0.06444936858428107
1104	8	0.45372137926269573
1104	9	0.32077400553671964
1104	10	0.37127555427337455
1104	11	0.14562370741676034
1104	12	0.2573776869008313
1104	13	0.07182740101772112
1104	14	0.038284474614812325
1104	15	0.6101031967005852
1104	16	0.0829411567901488
1104	17	0.09531604905253488
1104	18	-0.004557809767298693
1104	19	-0.6701831735473576
1104	20	-0.03530293147831287
1104	21	0.06817546586992762
1104	22	0.24951514231827332
1104	23	0.3216200808415053
1104	24	1.5222515624899202
1104	25	0.733472724396492
1105	1	0.6139782618488674
1105	2	0.497761175158864
1105	3	0.8358633023594528
1105	4	0.6470311335140515
1105	5	0.5663475079084535
1105	6	0.44323751927610644
1105	7	0.9758274342966651
1105	8	1.1912876298603479
1105	9	0.2220822323095959
1105	10	0.9260664516192776
1105	11	0.7856604313564972
1105	12	0.6527119154437813
1105	13	0.6336655308434587
1105	14	0.2668452217079249
1105	15	1.092191374847303
1105	16	0.6185664301851261
1105	17	0.3726987816798726
1105	18	0.4786697260665207
1105	19	-0.11091368493584497
1105	20	0.5492307965610763
1105	21	0.7127645341398363
1105	22	0.8952511979026525
1105	23	0.5323173270546704
1105	24	-3.6802514639289363
1105	25	-1.3576758905718194
1106	1	-0.35504404557532254
1106	2	-0.9503116144310049
1106	3	-0.5734329100258151
1106	4	-1.1126121800679165
1106	5	-0.6662538630686541
1106	6	-0.39381371808882903
1106	7	-3.2192971735806424
1106	8	-1.0971113477160408
1106	9	-1.3019824824574577
1106	10	-0.4912537528654468
1106	11	-0.6669217691779536
1106	12	-0.3696536009963296
1106	13	-0.6687336791019334
1106	14	-0.9070829762704662
1106	15	-0.18866287169620932
1106	16	-0.4360983775295253
1106	17	-0.7046728344465407
1106	18	-0.8364662097996387
1106	19	-0.1298808047220632
1106	20	-0.32795492436375184
1106	21	-0.5130255204668828
1106	22	-1.0828480236611924
1106	23	-0.9279403120762737
1106	24	-3.881504804233341
1106	25	-2.165579341251501
1107	1	0.2722531440725042
1107	2	0.15891257460547337
1107	3	0.266274429865625
1107	4	0.2651967826768562
1107	5	0.3341351771136374
1107	6	0.24295243171016878
1107	7	-1.809828908144084
1107	8	-2.0001616080024505
1107	9	-0.46074561256360624
1107	10	-0.20407031115663443
1107	11	-0.4015894177079318
1107	12	-0.08545974783120869
1107	13	-0.40194011123294615
1107	14	-0.40888923425968215
1107	15	-1.9301074612588174
1107	16	-0.7557782530366666
1107	17	-0.42192977696122114
1107	18	-0.7528619377679178
1107	19	-0.14650300965361396
1107	20	-0.06705369425574759
1107	21	-0.38433890159657835
1107	22	-0.3851229824414588
1107	23	-0.5975583579830115
1107	24	-4.382515613992691
1107	25	-2.55621968286583
1108	1	0.4606673607722591
1108	2	0.2100067583618177
1108	3	0.6810914469832134
1108	4	0.8829734668973934
1108	5	0.8366133372947147
1108	6	-2.8397911211433886
1108	7	-1.2969209457943478
1108	8	0.3104143435347382
1108	9	0.17373857585174335
1108	10	0.5640534066190313
1108	11	0.5061109339390626
1108	12	0.5098955956198097
1108	13	0.5055551984317077
1108	14	-0.04834227338055541
1108	15	-1.007039213034993
1108	16	0.0705728312367313
1108	17	0.24543858893392212
1108	18	0.3104778088229736
1108	19	0.28817687994266805
1108	20	-0.9502048250053221
1108	21	-0.25726982864833303
1108	22	0.0062622925824911135
1108	23	0.23874409716690081
1108	24	-0.6801443034668608
1108	25	-0.31703707133723213
1109	1	0.9617700562754976
1109	2	1.1454540285347612
1109	3	-0.09632940867200546
1109	4	1.0744821413593293
1109	5	1.25886019056437
1109	6	1.6772704405106325
1109	7	1.739720017918188
1109	8	2.0692307760924216
1109	9	1.2065993579787317
1109	10	1.537792832943687
1109	11	1.3952096428616656
1109	12	1.0868391106983424
1109	13	1.2109165564234559
1109	14	1.3033320676819073
1109	15	-0.22309455078823248
1109	16	0.9208616758809569
1109	17	0.9518889585136634
1109	18	1.0381907020359762
1109	19	-0.40512096643713846
1109	20	0.7233437107017124
1109	21	1.0029651697894195
1109	22	1.2231124377460247
1109	23	1.532634685141936
1109	24	0.5054916907037921
1109	25	0.9907529535446855
1110	1	0.4500490442922036
1110	2	0.5319431295718609
1110	3	0.786820401825242
1110	4	0.48790697496251667
1110	5	0.5217160617433763
1110	6	1.0118432815531335
1110	7	0.7798301089113018
1110	8	0.9679620500641235
1110	9	0.4650690750076336
1110	10	0.5820097630672478
1110	11	0.36248209763193273
1110	12	0.3827631551798234
1110	13	0.4661571193227019
1110	14	0.21751423103350476
1110	15	0.7995890080030392
1110	16	0.3179295494307368
1110	17	0.06047664639843475
1110	18	0.274391291095462
1110	19	-2.1953122404542533
1110	20	-0.1600127474930062
1110	21	-0.24767464327389632
1110	22	0.10816522374303617
1110	23	0.34830834528140997
1110	24	-0.2161584025147576
1110	25	-0.4402392051970807
1111	1	0.4301487687288059
1111	2	0.26556403715803256
1111	3	0.7687389133024745
1111	4	0.8718757970634137
1111	5	0.8850605251382859
1111	6	0.956390866855962
1111	7	0.656610146636833
1111	8	0.9595418667898699
1111	9	0.35444793063597757
1111	10	0.2585710887914132
1111	11	0.09061003957716451
1111	12	0.12535788939098325
1111	13	0.15940095460693726
1111	14	0.342006200345196
1111	15	-0.6556945683499862
1111	16	-0.04245452348546763
1111	17	-0.05445353620092419
1111	18	0.07215017941621937
1111	19	0.6200351192732256
1111	20	0.12860930970384743
1111	21	0.3420905044196161
1111	22	0.5564378908054906
1111	23	0.5049985646683055
1111	24	0.8969724391947196
1111	25	0.47199002145238467
1112	1	0.11346472635450056
1112	2	0.2812234732882928
1112	3	-1.4641785240609018
1112	4	-0.1398051625240555
1112	5	-0.10625386306865536
1112	6	0.8907476854199423
1112	7	0.9705712474719874
1112	8	1.004116722459397
1112	9	-0.18908774561535324
1112	10	0.43655326467841604
1112	11	0.47597296766415553
1112	12	0.5113990305826164
1112	13	0.004073338441926921
1112	14	0.26554860267690117
1112	15	0.5977844967248416
1112	16	0.4312700435231065
1112	17	-0.06392722041145049
1112	18	0.13239343932317382
1112	19	0.8913472654533692
1112	20	0.7201591107239693
1112	21	0.7049569356734722
1112	22	0.7620642570405636
1112	23	0.6819281089763578
1112	24	1.7415215115561296
1112	25	0.9992452201520123
1113	1	-0.6371766012099869
1113	2	-0.07288244707974201
1113	3	-4.87802813247319
1113	4	-1.2677639773550986
1113	5	-0.05614378036971246
1113	6	0.6819662625599854
1113	7	0.9214656009415094
1113	8	1.2984515710272255
1113	9	0.6617135030839165
1113	10	0.7394931847178512
1113	11	0.44356484016538644
1113	12	0.1830875129748648
1113	13	-0.0644517724622844
1113	14	-0.41373722896776854
1113	15	0.20238058632449452
1113	16	0.5434733405273278
1113	17	0.273330022591999
1113	18	0.23600261835817093
1113	19	1.1866252105763238
1113	20	0.6811011119288537
1113	21	0.42892319888613173
1113	22	0.6143880425709867
1113	23	0.37405801751461354
1113	24	1.4044055687334165
1113	25	1.0638577400052327
1114	1	-0.13168075931088197
1114	2	-0.2353191026637802
1114	3	0.16247316614020946
1114	4	-0.22525659598575842
1114	5	0.2556545665419563
1114	6	0.7612461877733878
1114	7	0.7823341914172168
1114	8	0.2509845016635026
1114	9	-0.1735592903308003
1114	10	-0.17936885770070177
1114	11	-0.22532998483905686
1114	12	-0.8169685346719788
1114	13	-0.4743070637831508
1114	14	-0.36942786287722384
1114	15	-2.9584264574899635
1114	16	-0.75857377333611
1114	17	-0.6916486581521468
1114	18	-0.37930959875983916
1114	19	0.36279142463180847
1114	20	0.1539930859058245
1114	21	-0.19167335957684628
1114	22	-0.028879260289343733
1114	23	-0.6727787801977954
1114	24	0.6652164195578436
1114	25	0.3288044841657012
1115	1	-3.8871058395260754
1115	2	-1.203269212431941
1115	3	-0.5284962917739513
1115	4	0.25790424452472394
1115	5	0.5136097425328856
1115	6	1.3702031490047197
1115	7	1.074552714492654
1115	8	1.2468289927493643
1115	9	0.5691911861353915
1115	10	-0.9076926617102572
1115	11	0.0866377602093138
1115	12	-0.32152511955533924
1115	13	0.1917353718637571
1115	14	0.10176704195016129
1115	15	0.5353824976619066
1115	16	0.09438212640092303
1115	17	-0.11281159752634551
1115	18	0.017176613876763724
1115	19	-1.8292274651416598
1115	20	-0.788933502113764
1115	21	-0.5326452299780442
1115	22	-0.2585208853406087
1115	23	-0.32595361313306626
1115	24	-1.8004327807027032
1115	25	-1.135756081321258
1116	1	-0.11528228911316063
1116	2	-0.7503284835268218
1116	3	-0.07045486580283011
1116	4	-0.5899556867636484
1116	5	0.08372018447624185
1116	6	0.8524750808315495
1116	7	0.5407529146577055
1116	8	-2.4994003542560606
1116	9	-1.1093244320058844
1116	10	-1.3951647878493532
1116	11	-0.713934641595678
1116	12	-0.653158220533335
1116	13	-0.9168131974243678
1116	14	-1.0262566501000094
1116	15	-1.9872004508511978
1116	16	-1.2407802004299668
1116	17	-0.8522839109543767
1116	18	-1.1586789161216515
1116	19	-0.32034587271749615
1116	20	-0.6301388234606051
1116	21	-0.9071265274221361
1116	22	-0.8507811701368482
1116	23	-0.6122481600986944
1116	24	-0.17904321386689048
1116	25	-0.66491755364639
1201	1	0.23164295882091324
1201	2	-0.08119979800469324
1201	3	0.28599280590335496
1201	4	0.15197346437104997
1201	5	0.14497502883322966
1201	6	0.45891235939997443
1201	7	0.46676994092693747
1201	8	0.9178748883659651
1201	9	0.540045743846008
1201	10	0.5741916228689732
1201	11	0.40144073544426107
1201	12	0.6949957272423006
1201	13	0.5335445574731139
1201	14	0.5580963655266515
1201	15	0.9558197487894304
1201	16	0.6334813093926996
1201	17	0.4631108666093837
1201	18	0.3762120021236157
1201	19	-0.21756928822590993
1201	20	0.5109122230128875
1201	21	0.6217941105779445
1201	22	0.9103521899876839
1201	23	0.882679988675612
1201	24	0.2534406536737208
1201	25	0.5053243524088842
1202	1	-0.18153496368331545
1202	2	-0.43674689432685554
1202	3	-0.765853559706553
1202	4	-0.8605664296494666
1202	5	-0.4533718346761191
1202	6	0.3522032678388882
1202	7	0.014177967452152274
1202	8	0.6373285506163597
1202	9	0.4629499457862991
1202	10	0.7507569718261422
1202	11	0.408360296410045
1202	12	0.7784773270304512
1202	13	0.49054038946169953
1202	14	0.6413021207476959
1202	15	1.1367393042383256
1202	16	0.9708819708679712
1202	17	0.7724015874739862
1202	18	0.6840145415486987
1202	19	0.2375328708095168
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2211	1	1.1390913335891995
2211	2	0.9992747905064192
2211	3	0.9116555235989665
2211	4	1.2458511606944107
2211	5	1.3227740813853117
2211	6	-1.4492454570453397
2211	7	0.7435971346655421
2211	8	1.0028515073042463
2211	9	1.321031689780959
2211	10	0.9977384752618761
2211	11	1.2086925516403824
2211	12	1.1272033622587116
2211	13	1.3644253585573622
2211	14	1.2195528314899775
2211	15	1.782910503925251
2211	16	1.3339456249849064
2211	17	1.2908233367632462
2211	18	1.1274102974293498
2211	19	1.372533618959288
2211	20	1.1561977415028721
2211	21	0.9382788397822779
2211	22	-1.8811507838189137
2211	23	-0.4116429522271332
2211	24	1.7840725144705871
2211	25	1.507123384618552
2212	1	-0.7707869946059844
2212	2	-0.24318543776379542
2212	3	-3.3521009046777372
2212	4	-1.932552736918332
2212	5	-0.915047835641694
2212	6	-0.08841324471177003
2212	7	0.2489158731969603
2212	8	0.12086342959799623
2212	9	-0.19339258598610343
2212	10	0.20657765624435598
2212	11	-0.14260473302422838
2212	12	-0.19861452028735016
2212	13	-0.10461547938017901
2212	14	-0.29691440344809195
2212	15	-1.1237440414073063
2212	16	-0.41842804309405324
2212	17	-0.40460801611853725
2212	18	-0.3626035794854374
2212	19	0.25891623938149877
2212	20	-1.032226384424824
2212	21	-1.1560707623047364
2212	22	-0.2990024674431453
2212	23	0.07298453479889844
2212	24	1.098104524185544
2212	25	0.6595715251007874
2213	1	-0.9094799771764777
2213	2	-0.8582479901805051
2213	3	-0.2104943135345856
2213	4	-6.7259255267257
2213	5	-1.9188077160555537
2213	6	-3.959436636143458
2213	7	-1.716868237315932
2213	8	-3.4734160334810866
2213	9	-1.649889433379066
2213	10	-0.9169421828010442
2213	11	-0.872867805153966
2213	12	-0.7299689463216694
2213	13	-0.5789046762149681
2213	14	-0.8706628119355813
2213	15	-3.314953677825457
2213	16	-1.6651678867456
2213	17	-1.1873649286059873
2213	18	-0.994777335715689
2213	19	-0.18695386712490603
2213	20	-2.119496674308234
2213	21	-1.0545811500471558
2213	22	-1.2589528426707375
2213	23	-0.963763652071826
2213	24	-1.3614764897763116
2213	25	-1.098873811604598
2214	1	0.036498294526900565
2214	2	0.08951605513661676
2214	3	0.031631670717381155
2214	4	0.32297975251945843
2214	5	0.2863984369727852
2214	6	0.08480901985840106
2214	7	0.6741705015126023
2214	8	-2.22016011135743
2214	9	-1.2513712101822811
2214	10	-1.0459747087985853
2214	11	-0.692201503947949
2214	12	-2.1175814915889504
2214	13	-1.2204500763943784
2214	14	-1.0452408710073087
2214	15	-2.4375491128897444
2214	16	-1.0431746311557148
2214	17	-0.8034692842324205
2214	18	-0.9055883261140423
2214	19	0.1084463122830428
2214	20	-1.76232949267429
2214	21	-1.3793053933775
2214	22	-1.0445312672031193
2214	23	-1.5810432959138154
2214	24	-2.6136069592271234
2214	25	-1.988396719342397
2215	1	0.5461685932654277
2215	2	0.13340647076316614
2215	3	0.35347558298470005
2215	4	0.6614920598357017
2215	5	0.7982705712532763
2215	6	1.5540333219006115
2215	7	0.7881946568892282
2215	8	-0.6237200849872551
2215	9	0.02771375955868649
2215	10	0.030083987062930362
2215	11	-0.022983312971582848
2215	12	-0.46023636081213937
2215	13	-0.3355612607559735
2215	14	-0.33675074871192123
2215	15	-0.12556253501254133
2215	16	0.23692236812577505
2215	17	-0.35454760957815523
2215	18	-1.0858369909387449
2215	19	-0.04518588303402993
2215	20	-4.1545886240881345
2215	21	-2.1145360583355384
2215	22	-1.138012982153377
2215	23	-1.0169974740310153
2215	24	-1.1015839001386745
2215	25	-0.8643939341778406
2216	1	-0.7786703282631748
2216	2	-1.2432651066521245
2216	3	-0.2293622380628868
2216	4	-0.4064883800017114
2216	5	-0.5981803774678625
2216	6	-2.024714082206671
2216	7	-10.2000940951298
2216	8	-11.169885263637923
2216	9	-6.503993405992709
2216	10	-4.000944252699934
2216	11	-2.9338780750369367
2216	12	-2.3565950111254246
2216	13	-2.0492533745670123
2216	14	-2.1287314370450474
2216	15	-3.254886804168901
2216	16	-2.2335661034480943
2216	17	-2.7403025928046993
2216	18	-2.3528101356520033
2216	19	-1.7108970245168358
2216	20	-2.642432746277027
2216	21	-5.125877225200412
2216	22	-3.648471989235503
2216	23	-3.504516776823042
2216	24	-3.8006334040347305
2216	25	-2.9659186328767895
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	36209.0
3	7.0
4	322.0
5	1654.0
6	2226.0
7	1858.0
8	1370.0
9	1248.0
10	1088.0
11	1155.0
12	1047.0
13	1080.0
14	1101.0
15	1163.0
16	1248.0
17	1323.0
18	1572.0
19	1927.0
20	2446.0
21	3160.0
22	4094.0
23	4817.0
24	5784.0
25	6546.0
26	7447.0
27	7954.0
28	9158.0
29	10873.0
30	12870.0
31	15770.0
32	20343.0
33	26507.0
34	35654.0
35	49247.0
36	70273.0
37	109735.0
38	133354.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.95571925191925	18.745747430730606	12.718115649623748	25.580417667726397
2	14.01958551582783	20.13107619119917	42.665721973396764	23.183616319576235
3	18.329321144376383	24.795833846454908	30.484482360145552	26.390362649023157
4	11.668245582687438	17.641605167606713	39.13514734489257	31.555001904813277
5	12.500379115935337	37.84267868155272	37.11090067836478	12.546041524147158
6	30.633736368905602	37.624179438446085	18.82489519727176	12.917188995376549
7	26.936726230511066	32.36658971923765	22.923348869278374	17.773335180972918
8	24.002696761351828	38.3144565671039	21.19019095300775	16.49265571853652
9	26.02213258659442	15.732232095620322	20.426982345825035	37.818652971960226
10	14.21919867344325	29.62778219663777	35.203803065640294	20.949216064278684
11	33.73376240459623	23.095041531177866	24.80059980118949	18.37059626303641
12	22.665285078851596	25.151469200700905	32.53403423642	19.649211484027497
13	28.5506091427859	22.502649362552418	25.868898252684325	23.07784324197736
14	20.350957002291416	22.607494271465157	27.940591723952014	29.100957002291416
15	22.738224005876624	31.49587726480866	23.39479527848259	22.371103450832127
16	21.578419726876774	28.55275018744577	28.2888939436062	21.579936142071254
17	21.237986763536373	28.93623043847471	28.186119236005336	21.639663561983582
18	21.076121366919445	28.288115860962805	29.8484177113888	20.78734506072895
19	23.578722113634832	27.291568376356405	27.25483588326481	21.874873626743952
20	23.063820180042356	27.98929113587082	28.18742871006711	20.759459974019716
21	22.64232004973557	27.27992600317755	27.186419089562776	22.891334857524107
22	22.458679426482234	28.54413424763702	27.801627550418683	21.195558775462068
23	22.082173593654176	28.12556863749402	27.829540170237426	21.96271759861438
24	23.08793921167244	27.370753199053134	28.148297909980037	21.393009679294398
25	22.404648702197992	27.645389879738925	28.238102779584256	21.711858638478827
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	219.0
1	219.0
2	267.0
3	315.0
4	315.0
5	315.0
6	445.5
7	576.0
8	576.0
9	576.0
10	895.0
11	1214.0
12	1214.0
13	1214.0
14	2024.0
15	2834.0
16	2834.0
17	2834.0
18	5066.0
19	7298.0
20	7298.0
21	7298.0
22	11655.0
23	16012.0
24	16012.0
25	16012.0
26	22894.5
27	29777.0
28	29777.0
29	29777.0
30	39066.5
31	48356.0
32	48356.0
33	48356.0
34	54682.5
35	61009.0
36	61009.0
37	61009.0
38	65713.5
39	70418.0
40	70418.0
41	70418.0
42	73185.5
43	75953.0
44	75953.0
45	75953.0
46	76478.0
47	77003.0
48	77003.0
49	77003.0
50	73522.5
51	70042.0
52	70042.0
53	70042.0
54	61353.5
55	52665.0
56	52665.0
57	52665.0
58	44738.5
59	36812.0
60	36812.0
61	36812.0
62	29770.5
63	22729.0
64	22729.0
65	22729.0
66	17291.0
67	11853.0
68	11853.0
69	11853.0
70	8642.0
71	5431.0
72	5431.0
73	5431.0
74	3748.5
75	2066.0
76	2066.0
77	2066.0
78	1421.5
79	777.0
80	777.0
81	777.0
82	489.0
83	201.0
84	201.0
85	201.0
86	129.5
87	58.0
88	58.0
89	58.0
90	32.5
91	7.0
92	7.0
93	7.0
94	4.5
95	2.0
96	2.0
97	2.0
98	2.5
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.22589828681165036
2	0.14234455805804963
3	0.14369219884439802
4	0.06670821892424575
5	0.02425753415427118
6	0.02257298317133568
7	0.3173694051850479
8	0.3047352728130317
9	0.019540791402051783
10	0.03604939103481967
11	0.016845509829354986
12	0.018530060812290486
13	0.015329413944713035
14	0.018530060812290486
15	0.015497869043006585
16	0.02105688728669373
17	0.019877701598638883
18	0.015329413944713035
19	0.025268264744032477
20	0.017350875124235637
21	0.015329413944713035
22	0.016845509829354986
23	0.017856240419116286
24	0.016003234337887234
25	0.014824048649832387
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	593630.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.83870022886498
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.00825707569282	82.57563112309857
2	4.6584446867803395	8.183834527496941
3	0.7029094400223944	1.8522795477049931
4	0.2141683915468686	0.7524909257433429
5	0.1153567552120724	0.5066393720223893
6	0.06925239640435027	0.364982429273535
7	0.038623743555975865	0.23748616023509433
8	0.027755584800609287	0.19504115943820483
9	0.023759845028529517	0.18783305144507628
>10	0.11805103958381673	1.9393329622641886
>50	0.011518592324263725	0.6750237722787715
>100	0.010750599140229529	1.7200089789570185
>500	9.598749231225105E-4	0.6365719505016907
>1k	1.919749846245021E-4	0.17284403954019417
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	1025	0.17266647575088861	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	801	0.13493253373313344	No Hit
GTATCAACGCAGAGTACTTTTTTTT	792	0.1334164378484915	No Hit
CTGTAGGACGTGGAATATGGCAAGA	792	0.1334164378484915	No Hit
GTCCTACAGTGTGCATTTCTCATTT	727	0.12246685645941074	No Hit
CTTTAGGACGTGAAATATGGCGAGG	663	0.11168573016862357	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.6845509829354987E-4	0.0
4	0.0	0.0	0.0	1.6845509829354987E-4	0.0
5	0.0	0.0	0.0	1.6845509829354987E-4	0.0
6	0.0	0.0	0.0	1.6845509829354987E-4	0.0
7	0.0	0.0	0.0	1.6845509829354987E-4	0.0
8	0.0	0.0	0.0	1.6845509829354987E-4	0.0
9	0.0	0.0	0.0	1.6845509829354987E-4	0.0
10	1.6845509829354987E-4	0.0	0.0	3.3691019658709973E-4	0.0
11	1.6845509829354987E-4	0.0	0.0	3.3691019658709973E-4	0.0
12	1.6845509829354987E-4	0.0	0.0	3.3691019658709973E-4	0.0
13	1.6845509829354987E-4	0.0	0.0	3.3691019658709973E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	210	0.0	14.055971	4
TCCTATA	95	4.611138E-9	14.055893	2
GAATAGT	60	4.0272437E-4	12.688201	6
AATGTCC	90	5.330585E-7	12.677481	8
TGGCGAA	60	4.1495363E-4	12.643298	18
AGGACCT	310	0.0	12.2799425	5
GTAGGAC	520	0.0	11.921398	3
GACGTGG	225	0.0	11.842321	7
AGGACGT	430	0.0	11.730202	5
CTAGAGT	130	2.5065674E-9	11.719123	4
TGAATAG	65	7.891586E-4	11.713177	5
GTCCTAG	110	4.6428613E-7	11.282614	1
GTCCTAC	500	0.0	11.265257	1
GGACGTG	465	0.0	11.255662	6
CTTAAAC	60	0.005700436	11.126638	3
TACTGGG	60	0.0057946346	11.102176	7
GGCGAAA	60	0.005949615	11.062886	19
CATTTCT	670	0.0	11.039298	14
TCCTACA	550	0.0	10.9252615	2
TGTAGGA	585	0.0	10.9237795	2
>>END_MODULE