FastQCFastQC Report
Thu 26 May 2016
SRR1926845_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926845_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences593630
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14120.2378585987904924No Hit
CTGTAGGACGTGGAATATGGCAAGA9870.1662651820157337No Hit
GTCCTACAGTGGACATTTCTAAATT9790.16491754122938532No Hit
GGTATCAACGCAGAGTACTTTTTTT9050.15245186395566263No Hit
CTTTAGGACGTGAAATATGGCGAGG7960.1340902582416657No Hit
GTCCTAAAGTGTGTATTTCTCATTT7860.1324057072587302No Hit
TATCAACGCAGAGTACTTTTTTTTT7680.12937351548944628No Hit
GTCCTACAGTGTGCATTTCTCATTT7320.12330913195087849No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTAG350.00211801516.3427471
AGATCTA400.005184991814.28665
TTAATCC500.001468960413.3341594
TCTGGAC801.934999E-613.09715753
TAGGACC2700.013.0519554
GGACCAT604.0007645E-412.6981266
GGCGAGG2750.012.41894119
TCCTATA1001.374101E-712.39219952
ACAGTAC701.06209714E-412.2466943
GGTATCA3050.012.1900811
GTCTTAA802.7588258E-511.9165861
GGACGTG5600.011.7344296
GTCCTAT657.7789795E-411.73325351
ATAGGAC907.2223884E-611.6419173
TATAGTG907.229195E-611.6409335
GTGTAGG1001.8435439E-611.4399221
CTTACAC752.0193585E-411.4302473
AGGACGT5800.011.3307515
TGTAGGA6150.011.3149552
TTAGGAC3650.011.2214753
Produced by FastQC (version 0.11.5)