FastQCFastQC Report
Thu 26 May 2016
SRR1926844_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926844_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614722
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT10600.17243567010778857No Hit
GTCCTAAAGTGTGTATTTCTCATTT9940.16169910951617156No Hit
CTGTAGGACGTGGAATATGGCAAGA9490.15437872729461447No Hit
GTCCTACAGTGTGCATTTCTCATTT9140.14868509667784788No Hit
GTATCAACGCAGAGTACTTTTTTTT8320.1353457335185661No Hit
CTTTAGGACGTGAAATATGGCGAGG8130.13225490546946425No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGTT350.002199074716.25126611
GTACTAA508.326243E-515.2784721
TGCCGGT400.005346019314.22101610
GTCCTAG852.5264671E-713.4810061
TAACCCT500.001465827613.3380584
GTATTAA905.0608287E-712.7320611
CTAGGAC701.0496762E-412.2622713
TAGACCT701.060154E-412.2492384
AATCCCG550.003113804212.06533419
TATGTAC802.7472086E-511.9216533
ATACTCT907.2727307E-611.6348266
TAGGACC2300.011.5983114
TATGCTC752.0279652E-411.4251495
GTCCTAC6700.011.4018451
TGTAGGA6150.011.3207242
GTATAGA855.0389528E-511.2341711
TTAGCAC600.005699923311.1268763
TTGGACC700.001454081410.8882114
TAGGACA2200.010.8263464
TGGCGAG5100.010.7810718
Produced by FastQC (version 0.11.5)