##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926844_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 614722 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.41866404651208 31.0 28.0 31.0 22.0 34.0 2 28.553625866651917 31.0 29.0 33.0 22.0 34.0 3 28.154040362960817 31.0 28.0 31.0 16.0 34.0 4 31.80611235647984 35.0 32.0 37.0 19.0 37.0 5 31.953580642957306 35.0 32.0 37.0 19.0 37.0 6 31.158038918405392 35.0 32.0 37.0 17.0 37.0 7 31.214132567241776 35.0 32.0 37.0 17.0 37.0 8 31.11177247601355 35.0 32.0 37.0 15.0 37.0 9 33.2723117116355 37.0 33.0 39.0 17.0 39.0 10 33.505548849723944 37.0 34.0 39.0 18.0 39.0 11 33.68205465234691 38.0 34.0 39.0 22.0 39.0 12 33.57035700690719 38.0 34.0 39.0 19.0 39.0 13 33.693604588740925 38.0 34.0 39.0 22.0 39.0 14 34.72982583997319 39.0 35.0 41.0 22.0 41.0 15 34.30786111445499 39.0 34.0 41.0 17.0 41.0 16 34.40966973688919 39.0 34.0 41.0 17.0 41.0 17 34.64445066225058 39.0 34.0 41.0 21.0 41.0 18 34.69769586902697 39.0 35.0 41.0 21.0 41.0 19 34.067259671851666 39.0 34.0 41.0 11.0 41.0 20 34.34135267649442 39.0 34.0 41.0 18.0 41.0 21 34.31751751198102 39.0 34.0 41.0 18.0 41.0 22 34.3499061364324 39.0 34.0 41.0 17.0 41.0 23 34.356790549223874 39.0 34.0 41.0 15.0 41.0 24 33.05327611505689 39.0 32.0 41.0 2.0 41.0 25 33.62140447226551 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.1472392756495573 1101 2 -0.28749606770610114 1101 3 -1.2099292174285345 1101 4 -0.5699331330979334 1101 5 -0.42763242387874456 1101 6 -2.621984841973955 1101 7 -0.8634913138473799 1101 8 -0.33865502070051434 1101 9 -0.3768521551091695 1101 10 -9.454145879395313 1101 11 -4.175256070914916 1101 12 -1.8271488979066497 1101 13 -1.0054847343587312 1101 14 -0.8472725319090202 1101 15 -2.110351803363045 1101 16 -0.43670215104832977 1101 17 -0.3726878536672942 1101 18 -0.17424157348431635 1101 19 -1.1820478446694267 1101 20 -0.3584944843687268 1101 21 -0.30286105027420973 1101 22 0.1448718151878623 1101 23 -0.022116805763182867 1101 24 -0.061152570902862635 1101 25 0.07111325555722203 1102 1 -0.7211719000705124 1102 2 -0.7159755321067713 1102 3 -1.0113628074732794 1102 4 -1.2313709797700128 1102 5 -0.8326794175700023 1102 6 -2.134146446669906 1102 7 -0.7073230141597939 1102 8 -0.45934748463085384 1102 9 -0.523058107555805 1102 10 -0.5350940562713191 1102 11 -0.6263889381212806 1102 12 -0.5206874952691152 1102 13 -0.5457978854979046 1102 14 -0.8756605576484802 1102 15 -0.5061261810940536 1102 16 -0.7723293335551702 1102 17 -0.7630639345747454 1102 18 -0.8117083021611151 1102 19 -0.37120237863442185 1102 20 -0.5045966486798008 1102 21 -0.7473711834120564 1102 22 -0.6043589702124308 1102 23 -1.0021316776833729 1102 24 -4.464746530696125 1102 25 -2.7226413262291445 1103 1 0.44614188850934866 1103 2 0.24085118615368373 1103 3 0.4514094258495831 1103 4 0.21466802703202603 1103 5 0.03794591072009368 1103 6 0.8767574035954979 1103 7 0.49894082681450413 1103 8 0.7890496678567622 1103 9 0.6442662390974121 1103 10 0.8092258322354269 1103 11 0.4717100807697534 1103 12 0.4018347228765151 1103 13 0.45428261981148665 1103 14 0.35030596797992075 1103 15 0.6695179273690357 1103 16 0.5756713632776709 1103 17 0.2686274423827868 1103 18 0.3759601321030033 1103 19 -1.3329267231760298 1103 20 -0.12047414335457063 1103 21 0.01727748349920688 1103 22 0.20977142727263498 1103 23 0.44862942743016987 1103 24 1.836269419913208 1103 25 0.967166437650107 1104 1 -0.40460889016203083 1104 2 -0.48817633981494524 1104 3 -1.5602693534829548 1104 4 -0.5982778042997481 1104 5 -0.5704895667358869 1104 6 -1.9904655675975356 1104 7 -0.5176863251852453 1104 8 -0.05294073498622964 1104 9 -0.011772790029802138 1104 10 0.007305367770221949 1104 11 -0.00745561740017564 1104 12 0.12409826762623055 1104 13 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1107 11 -0.8118290000514463 1107 12 -0.4594435438999298 1107 13 -0.6175169726140552 1107 14 -0.7747627411996234 1107 15 -1.8934436843022837 1107 16 -1.259379799557685 1107 17 -0.9322277918765138 1107 18 -1.1877203756128267 1107 19 0.05388495486415934 1107 20 0.08861179052619406 1107 21 -0.06194150015701183 1107 22 -0.3147658509077331 1107 23 -0.6090814827474347 1107 24 -4.828208954397599 1107 25 -2.8138247027662544 1108 1 0.7545093589990621 1108 2 0.32585565009236106 1108 3 0.943456783645459 1108 4 0.8171442762870527 1108 5 1.1527421595982155 1108 6 -2.234480650362805 1108 7 -0.8302210598650532 1108 8 0.5823375059206128 1108 9 0.502913677456192 1108 10 0.9169940277912758 1108 11 0.7603478845590317 1108 12 0.9187022864140246 1108 13 0.7650846447200337 1108 14 0.7589106514642907 1108 15 -0.48720754393573174 1108 16 0.8713548876927106 1108 17 0.6667742600303015 1108 18 0.5536402031491221 1108 19 0.7031876007669382 1108 20 -0.3260379305648584 1108 21 0.25997794261353135 1108 22 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2216 15 -3.105317021088556 2216 16 -2.2983174483715416 2216 17 -2.362180350041406 2216 18 -2.1761207670291185 2216 19 -1.602046590525184 2216 20 -2.899008400312745 2216 21 -5.606052442799108 2216 22 -3.802867792103168 2216 23 -3.5364636090102053 2216 24 -3.5350208574384077 2216 25 -2.7954326491561368 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32657.0 3 16.0 4 252.0 5 1374.0 6 2140.0 7 1746.0 8 1321.0 9 1170.0 10 1072.0 11 1015.0 12 959.0 13 1018.0 14 949.0 15 1074.0 16 1189.0 17 1278.0 18 1473.0 19 1735.0 20 2375.0 21 3057.0 22 3789.0 23 4505.0 24 5463.0 25 6232.0 26 7328.0 27 7890.0 28 8856.0 29 10549.0 30 12520.0 31 15843.0 32 20142.0 33 26788.0 34 35781.0 35 50412.0 36 72562.0 37 115472.0 38 152720.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.13666657426085 18.713971922763957 12.775810983355537 25.37355051961966 2 14.012925634429694 20.19585578159034 42.723995184248125 23.067223399731844 3 18.49736880692093 24.96554196060542 30.183776210104433 26.353313022369214 4 11.63541003673963 17.424384240863517 39.09523166152008 31.84497406087677 5 12.53709826484256 37.92060971323692 36.96044804290381 12.581843979016702 6 30.781333897395015 37.37288273483135 18.890154411884346 12.955628955889292 7 26.8788023185984 32.36915454711611 22.907419253766417 17.844623880519073 8 24.147728199956266 38.300743830515 20.981144761363897 16.570383208164838 9 26.06961790528008 15.859599226829285 20.531327081150387 37.53945578674025 10 14.495571995352305 29.45998196902187 34.952920920816666 21.091525114809162 11 33.468157871616235 22.98145538913095 24.98771610703094 18.562670632221874 12 22.966385734274837 25.245029449090495 32.1326998796004 19.655884937034266 13 28.691291819943416 22.5226925511162 25.758628375816954 23.02738725312343 14 20.376884503870695 22.902843717561154 27.879918908980862 28.84035286958729 15 22.76073689502516 31.656614597654208 23.20864646057078 22.37400204674985 16 21.465494097835194 28.5354007858706 28.576402730208834 21.422702386085373 17 21.169522135988675 29.08494817851971 28.123525487707653 21.622004197783962 18 21.112282551396945 28.094880155150758 30.051510832568646 20.74132646088366 19 23.38928942756867 27.21786429630208 27.39473046214625 21.998115813983006 20 23.230538444767678 27.898448151872206 28.31219839185711 20.558815011503004 21 22.64612330895572 27.297989863902966 27.087458410276017 22.968428416865294 22 22.687787679156287 28.646783281295505 27.395922989438954 21.269506050109253 23 21.90288848429825 28.269008302785654 27.910571367673644 21.91753184524245 24 23.110514541387026 27.401260931462275 28.214927801504984 21.273296725645718 25 22.409018729906677 27.680140311601086 28.084447279022246 21.82639367946999 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 243.0 1 243.0 2 310.0 3 377.0 4 377.0 5 377.0 6 500.0 7 623.0 8 623.0 9 623.0 10 947.5 11 1272.0 12 1272.0 13 1272.0 14 2139.5 15 3007.0 16 3007.0 17 3007.0 18 5184.5 19 7362.0 20 7362.0 21 7362.0 22 11837.5 23 16313.0 24 16313.0 25 16313.0 26 23584.5 27 30856.0 28 30856.0 29 30856.0 30 40458.5 31 50061.0 32 50061.0 33 50061.0 34 56461.5 35 62862.0 36 62862.0 37 62862.0 38 67577.0 39 72292.0 40 72292.0 41 72292.0 42 75237.0 43 78182.0 44 78182.0 45 78182.0 46 79553.5 47 80925.0 48 80925.0 49 80925.0 50 77006.5 51 73088.0 52 73088.0 53 73088.0 54 63986.5 55 54885.0 56 54885.0 57 54885.0 58 46285.0 59 37685.0 60 37685.0 61 37685.0 62 30462.0 63 23239.0 64 23239.0 65 23239.0 66 17896.5 67 12554.0 68 12554.0 69 12554.0 70 9068.0 71 5582.0 72 5582.0 73 5582.0 74 3883.0 75 2184.0 76 2184.0 77 2184.0 78 1479.0 79 774.0 80 774.0 81 774.0 82 518.0 83 262.0 84 262.0 85 262.0 86 166.5 87 71.0 88 71.0 89 71.0 90 43.5 91 16.0 92 16.0 93 16.0 94 9.5 95 3.0 96 3.0 97 3.0 98 3.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24157261331138302 2 0.14689566991257838 3 0.15161324956647068 4 0.06490738903113928 5 0.022449172146108323 6 0.021473121183234047 7 0.3152644610083908 8 0.3168912126131812 9 0.01789426765269504 10 0.03643923594730626 11 0.015616815405988398 12 0.016592866368862674 13 0.01512878992455126 14 0.016918216689820763 15 0.014478089282635078 16 0.01903299377604836 17 0.01821961797365313 18 0.015291465085030308 19 0.021635796343713095 20 0.016267516047904582 21 0.014152738961676986 22 0.01708089185029981 23 0.017731592492215995 24 0.015779490566467443 25 0.014640764443114122 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 614722.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.70766905980946 #Duplication Level Percentage of deduplicated Percentage of total 1 93.51155083702017 80.14657052408872 2 4.800178189491733 8.228241673861456 3 0.8272817723543867 2.127131770924872 4 0.2932737719249701 1.005432455522695 5 0.15457003945970163 0.6623918894286898 6 0.09147303052217655 0.4703964136735535 7 0.059366462338920976 0.35617127751771166 8 0.04534256067647351 0.3108964147826817 9 0.02970688645665679 0.22914971939020282 >10 0.15967665287857336 2.5377685662153486 >50 0.013885079509608624 0.8072812013716488 >100 0.01217308210398552 2.026009632217124 >500 0.0013314308548048586 0.9197575107418502 >1k 1.9020440782926553E-4 0.17280095026344583 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1060 0.17243567010778857 No Hit GTCCTAAAGTGTGTATTTCTCATTT 994 0.16169910951617156 No Hit CTGTAGGACGTGGAATATGGCAAGA 949 0.15437872729461447 No Hit GTCCTACAGTGTGCATTTCTCATTT 914 0.14868509667784788 No Hit GTATCAACGCAGAGTACTTTTTTTT 832 0.1353457335185661 No Hit CTTTAGGACGTGAAATATGGCGAGG 813 0.13225490546946425 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.626751604790458E-4 7 0.0 0.0 0.0 0.0 1.626751604790458E-4 8 0.0 0.0 0.0 0.0 1.626751604790458E-4 9 0.0 0.0 0.0 0.0 1.626751604790458E-4 10 0.0 0.0 0.0 0.0 1.626751604790458E-4 11 0.0 0.0 0.0 1.626751604790458E-4 1.626751604790458E-4 12 0.0 0.0 0.0 1.626751604790458E-4 1.626751604790458E-4 13 0.0 0.0 0.0 1.626751604790458E-4 1.626751604790458E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGGTT 35 0.0021990747 16.251266 11 GTACTAA 50 8.326243E-5 15.278472 1 TGCCGGT 40 0.0053460193 14.221016 10 GTCCTAG 85 2.5264671E-7 13.481006 1 TAACCCT 50 0.0014658276 13.338058 4 GTATTAA 90 5.0608287E-7 12.732061 1 CTAGGAC 70 1.0496762E-4 12.262271 3 TAGACCT 70 1.060154E-4 12.249238 4 AATCCCG 55 0.0031138042 12.065334 19 TATGTAC 80 2.7472086E-5 11.921653 3 ATACTCT 90 7.2727307E-6 11.634826 6 TAGGACC 230 0.0 11.598311 4 TATGCTC 75 2.0279652E-4 11.425149 5 GTCCTAC 670 0.0 11.401845 1 TGTAGGA 615 0.0 11.320724 2 GTATAGA 85 5.0389528E-5 11.234171 1 TTAGCAC 60 0.0056999233 11.126876 3 TTGGACC 70 0.0014540814 10.888211 4 TAGGACA 220 0.0 10.826346 4 TGGCGAG 510 0.0 10.78107 18 >>END_MODULE