FastQCFastQC Report
Thu 26 May 2016
SRR1926844_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926844_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614722
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16560.2693900657532998No Hit
GTCCTACAGTGGACATTTCTAAATT11300.18382293134132177No Hit
CTGTAGGACGTGGAATATGGCAAGA10710.17422509687305807No Hit
GGTATCAACGCAGAGTACTTTTTTT10380.16885681657724955No Hit
CTTTAGGACGTGAAATATGGCGAGG9960.16202445983712963No Hit
GTCCTAAAGTGTGTATTTCTCATTT9900.16104840887425537No Hit
GTCCTACAGTGTGCATTTCTCATTT9500.1545414024550935No Hit
TATCAACGCAGAGTACTTTTTTTTT8670.14103936413533272No Hit
CTGTAGGACCTGGAATATGGCGAGA7460.12135566971736818No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG400.005148360514.3019871
TAGGACC3250.013.1802864
AATGCCG802.0201514E-613.04723318
AATCCCG751.4929861E-512.65186119
CTAGACA701.0686013E-412.2388374
TTAGACT701.0686013E-412.2388374
GCACCGT550.003019051612.1152126
AGGACGT5100.011.9455325
TAGACAG1052.6396265E-711.7855475
GGACGTG5250.011.6042316
GACGTGG2800.011.5241067
ATAGGAC1001.8735336E-611.4247793
TGTAGGA7750.011.316712
GGCGAGG2100.011.29630519
GTAGGAC7450.011.2458693
GTCCTAG855.1170195E-511.2172431
TAGGAAC855.2029172E-511.1989364
GTATTAC600.005711805511.1237681
ATAAGGG600.00577468311.1074253
CTGTAGG7900.010.982961
Produced by FastQC (version 0.11.5)