FastQCFastQC Report
Thu 26 May 2016
SRR1926843_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926843_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677888
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15320.22599603474320243No Hit
CTGTAGGACGTGGAATATGGCAAGA11920.17584025679758308No Hit
GTCCTACAGTGTGCATTTCTCATTT11590.17097219599697885No Hit
GTCCTAAAGTGTGTATTTCTCATTT11200.16521903323262838No Hit
CTTTAGGACGTGAAATATGGCGAGG9320.13748583836858005No Hit
CTGTAGGACCTGGAATATGGCGAGA8630.1273071657854985No Hit
GTATCAACGCAGAGTACTTTTTTTT8170.1205213840634441No Hit
GTACATGGGAAGCAGTGGTATCAAC7340.10827747356495468No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACT402.801424E-416.5911719
AAGGCGT350.002136116116.3224286
CTTACAC508.445434E-515.2534793
CTCGAAC456.8604964E-414.74770718
TCCAACG1001.476401E-712.32486818
GTCTTAA701.0430953E-412.2709181
GGCGAGG2250.012.21952919
ATGCCGG701.1100563E-412.18943119
TAGGACA2750.012.120864
CTAGGGT550.003009075312.120864
GTCCTAC9200.012.0337981
GTGTTAC657.7080296E-411.7465181
GTCTAAG657.7080296E-411.7465181
ACTGTTC3000.011.7317778
TGTACTG1302.5120244E-911.7186655
GAACAGT2200.011.6853726
TAAAACC907.240762E-611.63987354
GATATAC2650.011.5248871
TATACAC3900.011.4889993
CCAACGA752.1100664E-411.37680219
Produced by FastQC (version 0.11.5)