##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926843_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 677888 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.47391456995846 31.0 28.0 31.0 22.0 34.0 2 28.634541399169184 31.0 30.0 33.0 22.0 34.0 3 28.23384836433157 31.0 28.0 31.0 16.0 34.0 4 31.876329128115557 35.0 32.0 37.0 19.0 37.0 5 32.03021148036254 35.0 32.0 37.0 19.0 37.0 6 31.271572885196374 35.0 32.0 37.0 17.0 37.0 7 31.31846558723565 35.0 32.0 37.0 17.0 37.0 8 31.216684762084594 35.0 32.0 37.0 16.0 37.0 9 33.34897652709592 37.0 33.0 39.0 17.0 39.0 10 33.57357557590635 38.0 34.0 39.0 19.0 39.0 11 33.7375805442787 38.0 34.0 39.0 23.0 39.0 12 33.62221340398414 38.0 34.0 39.0 19.0 39.0 13 33.742628575811935 38.0 34.0 39.0 23.0 39.0 14 34.79769666965635 39.0 35.0 41.0 23.0 41.0 15 34.38033273933157 39.0 34.0 41.0 17.0 41.0 16 34.47769690568354 39.0 34.0 41.0 18.0 41.0 17 34.70956116644638 39.0 34.0 41.0 22.0 41.0 18 34.76910492588746 39.0 35.0 41.0 22.0 41.0 19 34.153759913142 39.0 34.0 41.0 16.0 41.0 20 34.414046273130666 39.0 34.0 41.0 18.0 41.0 21 34.403718608383684 39.0 34.0 41.0 18.0 41.0 22 34.43918316890106 39.0 34.0 41.0 18.0 41.0 23 34.4386181788142 39.0 35.0 41.0 16.0 41.0 24 33.162782052492446 39.0 33.0 41.0 2.0 41.0 25 33.73557578833081 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.6297503598216245 1101 2 -0.8010912541877211 1101 3 -1.7800256375664922 1101 4 -1.0254842196774163 1101 5 -0.8066579101691644 1101 6 -2.8920579590036546 1101 7 -1.295394828061717 1101 8 -0.7081717015211417 1101 9 -0.6328019059009051 1101 10 -9.769558894708187 1101 11 -4.797154597450003 1101 12 -2.278806855992748 1101 13 -1.362377928962971 1101 14 -1.0566072712189722 1101 15 -2.4725274249411697 1101 16 -0.7373819643142951 1101 17 -0.6667021861232598 1101 18 -0.41618741888480315 1101 19 -1.414984326691048 1101 20 -0.5853455106379357 1101 21 -0.7964457247447427 1101 22 -0.45773071814378596 1101 23 -0.5576707785641304 1101 24 -0.8504755161251367 1101 25 -0.5427667185431062 1102 1 0.05322486217356115 1102 2 -0.18473362512591862 1102 3 -0.35536333676989074 1102 4 -0.25241533453956677 1102 5 -0.16004484386462892 1102 6 -1.0092733207743692 1102 7 0.044437602061094594 1102 8 0.5019601206796445 1102 9 0.5475440639187497 1102 10 0.4651362347237864 1102 11 0.37858647938000445 1102 12 0.3221454282626226 1102 13 0.33209789164715176 1102 14 0.068136255067202 1102 15 0.6045638141282907 1102 16 0.56346328843658 1102 17 0.47411404543707647 1102 18 0.35447008453132156 1102 19 0.7450245996518134 1102 20 0.6537017397523286 1102 21 0.4628190576597859 1102 22 0.1467973913752516 1102 23 -0.29547109077460476 1102 24 -3.7315446889365944 1102 25 -1.8312081621569654 1103 1 -0.04730151421488671 1103 2 -0.25256296881732965 1103 3 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-1.8497857318646922 2214 25 -0.930279890302856 2215 1 0.30569476185831235 2215 2 0.009025754309853085 2215 3 0.14593200802677941 2215 4 0.5562993813369523 2215 5 0.6195221850392727 2215 6 1.1425218336733032 2215 7 0.8234711238001857 2215 8 -0.44802799906974755 2215 9 0.023068539443229952 2215 10 -0.283550975501889 2215 11 -0.28225047323413577 2215 12 -0.5638315479000298 2215 13 -0.21727404499268488 2215 14 -0.27753310651728214 2215 15 -0.2901965978734964 2215 16 -0.07161251109545219 2215 17 -0.3508682225160129 2215 18 -1.2266269793243652 2215 19 0.2369376379839565 2215 20 -3.764272858593621 2215 21 -2.0127001181246413 2215 22 -1.0007191062738698 2215 23 -0.7906867504464969 2215 24 -0.9920313287335745 2215 25 -0.621803066810223 2216 1 -0.8135560278378193 2216 2 -1.4869212137018906 2216 3 -0.548244261048275 2216 4 -0.9637433289891568 2216 5 -0.9987631733270597 2216 6 -2.0477259751979844 2216 7 -10.414220738992892 2216 8 -11.786916640792398 2216 9 -6.748591379585111 2216 10 -4.215712740862035 2216 11 -3.039664718907492 2216 12 -2.6362967345352573 2216 13 -2.282620843942727 2216 14 -2.518348161907234 2216 15 -3.4476286395160685 2216 16 -2.485155243666526 2216 17 -2.859414736730546 2216 18 -2.633798754917194 2216 19 -1.9463608449096697 2216 20 -2.9766410571966375 2216 21 -5.17154693931964 2216 22 -3.951050556200464 2216 23 -3.8754845437463175 2216 24 -3.7186941396069138 2216 25 -3.2686776497171977 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35505.0 3 10.0 4 274.0 5 1573.0 6 2271.0 7 1831.0 8 1452.0 9 1245.0 10 1147.0 11 1081.0 12 1076.0 13 1073.0 14 1127.0 15 1136.0 16 1299.0 17 1401.0 18 1529.0 19 1952.0 20 2500.0 21 3327.0 22 4108.0 23 5034.0 24 5880.0 25 6834.0 26 7851.0 27 8675.0 28 9730.0 29 11509.0 30 13858.0 31 16927.0 32 21800.0 33 29216.0 34 38781.0 35 54809.0 36 79474.0 37 126376.0 38 174217.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.11145419269354 18.59679632784403 12.84352522241151 25.448224257050917 2 13.651272050350146 19.813255326064475 43.60414265874775 22.931329964837634 3 19.029621957687983 24.316038467618853 29.961925390312764 26.692414184380397 4 11.516182175692192 17.20961541868169 39.179701570093236 32.09450083553288 5 12.225165074329558 38.07901434947804 37.19724076874839 12.498579807444022 6 30.8736170828083 37.719075625540405 18.667500818905353 12.739806472745945 7 27.239142854605575 31.9762949982091 22.49784647991972 18.286715667265607 8 23.726839519327534 39.29926596815249 20.827561711951695 16.146332800568285 9 25.781064416512606 16.079266880212938 20.481556182784196 37.65811252049026 10 14.114258139634408 30.16508741354198 35.051893259115076 20.668761187708537 11 33.770794142598945 23.129062004352477 24.769576924495592 18.330566928552987 12 22.817803705982424 25.159161897342603 32.56330696741811 19.45972742925687 13 28.774823689102657 22.539762164714215 25.81176783026941 22.873646315913717 14 19.825574018171462 23.327225030467744 27.862418592847675 28.984782358513115 15 22.58941665609296 32.232782812172644 23.18990471862634 21.98789581310805 16 21.076844537430762 28.871367066120946 29.228426665564992 20.823361730883295 17 20.86271917658654 29.483123919240327 28.551088579505553 21.103068324667586 18 20.556900619221082 28.544513246786966 30.722778690060448 20.1758074439315 19 23.39192072405988 27.42846013332664 27.409426117031167 21.77019302558231 20 22.765177670367027 28.460308186233718 28.57745672586061 20.19705741753864 21 22.108109862612718 27.620420423964877 27.312803928048723 22.958665785373682 22 21.94985924470176 29.19433083691861 27.94553330618556 20.91027661219407 23 21.506115643950015 28.71918202340027 28.146568895052894 21.628133437596826 24 22.83257547367458 27.81187572478629 28.619357732381605 20.736191069157524 25 22.050617746604388 28.070216853107027 28.48568722281074 21.39347817747785 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 255.0 1 255.0 2 306.5 3 358.0 4 358.0 5 358.0 6 508.0 7 658.0 8 658.0 9 658.0 10 1012.5 11 1367.0 12 1367.0 13 1367.0 14 2248.0 15 3129.0 16 3129.0 17 3129.0 18 5525.5 19 7922.0 20 7922.0 21 7922.0 22 13014.5 23 18107.0 24 18107.0 25 18107.0 26 26392.0 27 34677.0 28 34677.0 29 34677.0 30 46334.5 31 57992.0 32 57992.0 33 57992.0 34 65263.5 35 72535.0 36 72535.0 37 72535.0 38 77375.0 39 82215.0 40 82215.0 41 82215.0 42 85295.5 43 88376.0 44 88376.0 45 88376.0 46 89422.0 47 90468.0 48 90468.0 49 90468.0 50 85019.5 51 79571.0 52 79571.0 53 79571.0 54 68575.5 55 57580.0 56 57580.0 57 57580.0 58 48117.0 59 38654.0 60 38654.0 61 38654.0 62 30947.0 63 23240.0 64 23240.0 65 23240.0 66 17758.5 67 12277.0 68 12277.0 69 12277.0 70 8855.5 71 5434.0 72 5434.0 73 5434.0 74 3747.5 75 2061.0 76 2061.0 77 2061.0 78 1389.0 79 717.0 80 717.0 81 717.0 82 464.0 83 211.0 84 211.0 85 211.0 86 136.0 87 61.0 88 61.0 89 61.0 90 38.0 91 15.0 92 15.0 93 15.0 94 9.0 95 3.0 96 3.0 97 3.0 98 4.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24546827794561932 2 0.15164746978851965 3 0.15622049660120846 4 0.07021808912386707 5 0.02404527001510574 6 0.022717617069486405 7 0.33191323640483383 8 0.3198168429003021 9 0.0187346582326284 10 0.03643669750755287 11 0.016669420317220542 12 0.017554522280966767 13 0.015931835347432026 14 0.017997073262839877 15 0.015636801359516617 16 0.01917720921450151 17 0.018292107250755286 18 0.014899216389728097 19 0.022717617069486405 20 0.017111971299093656 21 0.014161631419939579 22 0.017111971299093656 23 0.017407005287009062 24 0.0150467333836858 25 0.0150467333836858 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 677888.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.88253980690102 #Duplication Level Percentage of deduplicated Percentage of total 1 93.57573962165687 79.42946443395493 2 4.789958856967471 8.131677466999188 3 0.8148258456232387 2.074934618304191 4 0.2786724243339193 0.9461769260643806 5 0.13327248458475546 0.5656253488965055 6 0.0811527158615156 0.41330691807319353 7 0.05726981543017043 0.3402845171589721 8 0.04148555269268923 0.2817119262278786 9 0.03088299575533533 0.2359284404902746 >10 0.16383467422344752 2.5928859667307913 >50 0.017584378628297745 1.0334399974091777 >100 0.012883260615901894 2.237963705173734 >500 0.0017409811617449214 0.9772618839841387 >1k 6.963924646979686E-4 0.739337850532685 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1532 0.22599603474320243 No Hit CTGTAGGACGTGGAATATGGCAAGA 1192 0.17584025679758308 No Hit GTCCTACAGTGTGCATTTCTCATTT 1159 0.17097219599697885 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1120 0.16521903323262838 No Hit CTTTAGGACGTGAAATATGGCGAGG 932 0.13748583836858005 No Hit CTGTAGGACCTGGAATATGGCGAGA 863 0.1273071657854985 No Hit GTATCAACGCAGAGTACTTTTTTTT 817 0.1205213840634441 No Hit GTACATGGGAAGCAGTGGTATCAAC 734 0.10827747356495468 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.4751699395770392E-4 0.0 0.0 0.0 6 0.0 1.4751699395770392E-4 0.0 0.0 0.0 7 0.0 1.4751699395770392E-4 0.0 0.0 0.0 8 0.0 1.4751699395770392E-4 0.0 0.0 0.0 9 0.0 1.4751699395770392E-4 0.0 0.0 0.0 10 0.0 1.4751699395770392E-4 0.0 0.0 0.0 11 0.0 1.4751699395770392E-4 0.0 0.0 0.0 12 0.0 1.4751699395770392E-4 0.0 0.0 0.0 13 0.0 1.4751699395770392E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGAACT 40 2.801424E-4 16.59117 19 AAGGCGT 35 0.0021361161 16.322428 6 CTTACAC 50 8.445434E-5 15.253479 3 CTCGAAC 45 6.8604964E-4 14.747707 18 TCCAACG 100 1.476401E-7 12.324868 18 GTCTTAA 70 1.0430953E-4 12.270918 1 GGCGAGG 225 0.0 12.219529 19 ATGCCGG 70 1.1100563E-4 12.189431 19 TAGGACA 275 0.0 12.12086 4 CTAGGGT 55 0.0030090753 12.12086 4 GTCCTAC 920 0.0 12.033798 1 GTGTTAC 65 7.7080296E-4 11.746518 1 GTCTAAG 65 7.7080296E-4 11.746518 1 ACTGTTC 300 0.0 11.731777 8 TGTACTG 130 2.5120244E-9 11.718665 5 GAACAGT 220 0.0 11.685372 6 TAAAACC 90 7.240762E-6 11.6398735 4 GATATAC 265 0.0 11.524887 1 TATACAC 390 0.0 11.488999 3 CCAACGA 75 2.1100664E-4 11.376802 19 >>END_MODULE