FastQCFastQC Report
Thu 26 May 2016
SRR1926843_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926843_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677888
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15980.2357321563444109No Hit
GTATCAACGCAGAGTACTTTTTTTT15460.2280612726586103No Hit
CTGTAGGACGTGGAATATGGCAAGA14930.22024287197885198No Hit
GTCCTACAGTGTGCATTTCTCATTT11820.17436508685800603No Hit
CTTTAGGACGTGAAATATGGCGAGG11240.1658091012084592No Hit
GTCCTAAAGTGTGTATTTCTCATTT10970.1618261423716012No Hit
CTGTAGGACCTGGAATATGGCGAGA9450.1394035592900302No Hit
GGTATCAACGCAGAGTACTTTTTTT9200.1357156344410876No Hit
GTACATGGGAAGCAGTGGTATCAAC8580.12656958081570996No Hit
TATCAACGCAGAGTACTTTTTTTTT8010.11816111216012085No Hit
CCCATGTACTCTGCGTTGATACCAC6920.10208175981873112No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCGT250.005943288619.0469536
TAGGACC3700.016.216854
GCCGTGT400.005323390514.23041312
CCAGTAC707.054754E-613.6069813
CTAGGAC1500.013.3348423
GACGTGG4000.013.046477
CTAAGGT853.823752E-612.3254124
TGTAGGA9600.012.3166642
CCAACGA853.99501E-612.27721919
GTAGGAC9350.012.2244543
CTATAGG1251.3224053E-912.2063211
ATAGGAC1251.3460522E-912.1918563
GGCGAAA550.003097115612.0742919
ACAGTGC1201.0162694E-811.8595528
TCCAACG1052.7597525E-711.74573818
CGTGGAA4450.011.7262999
TAATACT657.8440225E-411.7220694
CAGTACC907.235296E-611.6406664
AGTACCA907.235296E-611.6406665
GGCGAGG2450.011.61666419
Produced by FastQC (version 0.11.5)