FastQCFastQC Report
Thu 26 May 2016
SRR1926842_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926842_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628544
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12910.20539532634151308No Hit
CTGTAGGACGTGGAATATGGCAAGA10120.16100702576112413No Hit
GTCCTAAAGTGTGTATTTCTCATTT10060.16005243865186847No Hit
GTCCTACAGTGTGCATTTCTCATTT9570.1522566439262804No Hit
CTTTAGGACGTGAAATATGGCGAGG8300.1320512167803686No Hit
GTATCAACGCAGAGTACTTTTTTTT6760.10755014764280624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2450.015.1561014
GTCGTAG400.00511236414.3171821
CGTGGTA400.00515601914.2988522
CTAGGGT604.0100547E-412.6948534
TGTAGGA6400.012.6604412
CTGTAGG6150.012.4159841
AATCCCG1155.4515112E-912.36747819
GCCGGTT1001.4735633E-712.32625311
TCCAACG1001.4735633E-712.32625318
CTACACT1700.012.3214754
TAATAGT701.0665307E-412.2414664
TCCTATA1251.3278623E-912.2016872
TAGGGTG550.003019740812.1149095
GTAGGAC6000.012.0745873
CCAACGA550.003109442812.06766119
CCTACAC1209.469659E-911.91570953
TATACTG1052.640827E-711.7852525
CCGGTTT1052.7782335E-711.73928912
TTTAAAC657.7848864E-411.732393
TCTTACA657.7848864E-411.732392
Produced by FastQC (version 0.11.5)