##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926842_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 628544 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.27747301700438 31.0 28.0 31.0 20.0 34.0 2 28.40787438906425 31.0 28.0 31.0 20.0 34.0 3 27.996741676000408 31.0 28.0 31.0 16.0 34.0 4 31.640632954892578 35.0 32.0 37.0 19.0 37.0 5 31.798195512167805 35.0 32.0 37.0 19.0 37.0 6 30.996055964260258 35.0 32.0 37.0 17.0 37.0 7 31.060307631605742 35.0 32.0 37.0 17.0 37.0 8 30.974081369005194 35.0 32.0 37.0 11.0 37.0 9 33.12178463242032 37.0 33.0 39.0 17.0 39.0 10 33.35926204052541 37.0 33.0 39.0 17.0 39.0 11 33.53895829090724 38.0 34.0 39.0 21.0 39.0 12 33.4137275990225 38.0 34.0 39.0 18.0 39.0 13 33.5450803762346 38.0 34.0 39.0 19.0 39.0 14 34.55884393137155 39.0 34.0 40.0 19.0 41.0 15 34.133764700641485 39.0 33.0 40.0 17.0 41.0 16 34.23018277161185 39.0 34.0 40.0 17.0 41.0 17 34.463353400875675 39.0 34.0 41.0 18.0 41.0 18 34.50561297220242 39.0 34.0 41.0 18.0 41.0 19 33.86973227013542 39.0 33.0 40.0 11.0 41.0 20 34.149082641788006 39.0 34.0 41.0 17.0 41.0 21 34.13978655432237 39.0 34.0 41.0 16.0 41.0 22 34.156165678138684 39.0 34.0 41.0 11.0 41.0 23 34.14819010284085 39.0 34.0 41.0 10.0 41.0 24 32.84120125241829 39.0 32.0 40.0 2.0 41.0 25 33.416589769371754 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.09563956181441213 1101 2 -0.1680257157851024 1101 3 -1.3069026667259749 1101 4 -0.48279403382992925 1101 5 -0.6175184047726674 1101 6 -2.3469874880552375 1101 7 -0.6936210872799684 1101 8 -0.2805332250458683 1101 9 -0.0948337657389402 1101 10 -8.90018020235394 1101 11 -4.140363339347829 1101 12 -1.6214336390910198 1101 13 -0.6959902837229492 1101 14 -0.35644764773912385 1101 15 -1.8010886094716483 1101 16 -0.17613972075494644 1101 17 -0.13115780912588093 1101 18 0.059554022462357636 1101 19 -0.541492152754067 1101 20 -0.14334360922502754 1101 21 -0.05730335480367188 1101 22 0.3023051754341566 1101 23 0.2192442292927268 1101 24 0.11764414009383017 1101 25 0.47345615559034826 1102 1 -0.6879766415738509 1102 2 -0.9894074568873386 1102 3 -0.9309857782314452 1102 4 -1.1106432894742184 1102 5 -0.9019707364299698 1102 6 -1.832812687700315 1102 7 -1.2565856934122763 1102 8 -0.5009315292951051 1102 9 -0.5259266376703167 1102 10 -0.2433232566771153 1102 11 -0.44956673084935517 1102 12 -0.32521210699992054 1102 13 -0.5755254311152385 1102 14 -0.6970939062425288 1102 15 -0.249925245575362 1102 16 -0.37813370675514335 1102 17 -0.47346531183710994 1102 18 -0.6741761362678034 1102 19 -0.15476485313857324 1102 20 -0.5293758482075788 1102 21 -0.5633025660823847 1102 22 -0.8509902006873062 1102 23 -1.3570078657481872 1102 24 -4.144963076705935 1102 25 -2.329927951675746 1103 1 -0.1894641097174521 1103 2 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0.5698235249062442 2215 2 0.19029058266603371 2215 3 0.32148989558238483 2215 4 0.8494588948594313 2215 5 0.7803823735904096 2215 6 1.478476384890751 2215 7 0.9086314482967275 2215 8 -0.13527809492557807 2215 9 0.11882493384436543 2215 10 -0.004079880001555125 2215 11 0.11297811831394 2215 12 -0.5301548435882282 2215 13 -0.09992337594717782 2215 14 0.11294656564243155 2215 15 0.06858274833950162 2215 16 0.25170641494754165 2215 17 -0.10669244235176478 2215 18 -1.2640897388396368 2215 19 0.006347693145762889 2215 20 -3.7331560944833058 2215 21 -2.2354659462023676 2215 22 -1.206726588069266 2215 23 -1.0648209487873075 2215 24 -1.2638815301632675 2215 25 -0.8959459200445252 2216 1 -0.9126205838257668 2216 2 -1.2518497027182889 2216 3 -0.590788876572752 2216 4 -0.7766203541223788 2216 5 -0.813271690920292 2216 6 -1.775841419765591 2216 7 -9.972834745209504 2216 8 -11.64496505206617 2216 9 -6.407225847873182 2216 10 -3.9783477375487735 2216 11 -2.892268916076457 2216 12 -2.5444386169241078 2216 13 -2.2355698901630205 2216 14 -2.303272334680088 2216 15 -3.455475324879597 2216 16 -2.4856345484752573 2216 17 -2.9222035429673667 2216 18 -2.4912957092013315 2216 19 -1.9674527189825817 2216 20 -2.701597867605681 2216 21 -5.680321943899106 2216 22 -4.292438932833715 2216 23 -4.3083341326890725 2216 24 -4.327589973618544 2216 25 -3.3128684146826544 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34388.0 3 6.0 4 269.0 5 1559.0 6 2086.0 7 1762.0 8 1379.0 9 1183.0 10 1089.0 11 1094.0 12 1045.0 13 1047.0 14 1102.0 15 1115.0 16 1265.0 17 1375.0 18 1520.0 19 1864.0 20 2379.0 21 3285.0 22 4052.0 23 4909.0 24 5747.0 25 6738.0 26 7795.0 27 8510.0 28 9581.0 29 11210.0 30 13723.0 31 16634.0 32 21104.0 33 27656.0 34 37759.0 35 52417.0 36 75188.0 37 116937.0 38 147772.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.32728934136435 18.452036378771712 12.702589348221647 25.518084931642292 2 13.931327203521748 19.749124900620256 42.98367065354031 23.335877242317686 3 18.999941047412218 24.05297447671612 29.99698863808369 26.950095837787973 4 11.586433748137203 17.035192520793267 38.70715568519061 32.671218045878916 5 12.585894657105664 37.72076398901312 36.93366815937974 12.75967319450147 6 31.482586589645052 37.01477573819015 18.53213345109366 12.970504221071142 7 27.454056512683835 31.846437434665688 22.255559900093363 18.443946152557114 8 24.160180531860394 38.75274699845035 20.62774400607731 16.459328463611946 9 25.834412784091814 15.889409076297808 20.176764703074127 38.09941343653625 10 14.313407798111111 29.76065802331985 34.86660958195529 21.059324596613752 11 34.361459203564465 22.698651390380714 24.424235191152484 18.515654214902337 12 23.014654282108854 24.799297281900394 32.23098674924453 19.95506168674622 13 29.041918549789713 22.246922106254765 25.501206970353245 23.209952373602277 14 20.215142738932563 22.66716527163362 27.70806148754018 29.409630501893634 15 22.882766478156956 31.56174515226641 23.087559950225796 22.467928419350834 16 21.531087534591656 28.5700418362102 28.61125742556926 21.28761320362888 17 21.34835751874973 29.027273638665264 28.102557410858214 21.52181143172679 18 21.019642413865533 28.32667129190562 29.957386273224323 20.696300021004525 19 23.69708823150286 27.1010740299277 27.016889434912894 22.18494830365654 20 23.038915366437784 28.131569106402615 28.182809130908716 20.646706396250885 21 22.572196019273488 27.31466452799684 27.07533719057712 23.037802262152546 22 22.49785175519557 28.65137965055218 27.535406256961902 21.31536233729035 23 22.0924940484526 28.1909205484335 27.569986378276536 22.146599024837364 24 23.224628917687195 27.42444687730733 28.16422460982254 21.18669959518293 25 22.614914086941024 27.581449526276764 28.072835942497605 21.730800444284608 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 175.0 1 175.0 2 234.0 3 293.0 4 293.0 5 293.0 6 410.5 7 528.0 8 528.0 9 528.0 10 835.0 11 1142.0 12 1142.0 13 1142.0 14 1916.5 15 2691.0 16 2691.0 17 2691.0 18 4687.0 19 6683.0 20 6683.0 21 6683.0 22 11140.0 23 15597.0 24 15597.0 25 15597.0 26 22725.5 27 29854.0 28 29854.0 29 29854.0 30 40076.5 31 50299.0 32 50299.0 33 50299.0 34 56957.0 35 63615.0 36 63615.0 37 63615.0 38 68482.5 39 73350.0 40 73350.0 41 73350.0 42 76871.0 43 80392.0 44 80392.0 45 80392.0 46 82267.5 47 84143.0 48 84143.0 49 84143.0 50 79918.0 51 75693.0 52 75693.0 53 75693.0 54 66512.0 55 57331.0 56 57331.0 57 57331.0 58 48560.0 59 39789.0 60 39789.0 61 39789.0 62 32243.0 63 24697.0 64 24697.0 65 24697.0 66 18861.5 67 13026.0 68 13026.0 69 13026.0 70 9448.5 71 5871.0 72 5871.0 73 5871.0 74 4047.0 75 2223.0 76 2223.0 77 2223.0 78 1517.0 79 811.0 80 811.0 81 811.0 82 532.0 83 253.0 84 253.0 85 253.0 86 158.5 87 64.0 88 64.0 89 64.0 90 39.5 91 15.0 92 15.0 93 15.0 94 9.0 95 3.0 96 3.0 97 3.0 98 4.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23466933102535387 2 0.1433471642398941 3 0.14652912127074638 4 0.07254862030343143 5 0.025137460543732816 6 0.023705579879849302 7 0.31167269117197843 8 0.3103999083596375 9 0.021955503512880562 10 0.03786528866714184 11 0.01893264433357092 12 0.020205427145911822 13 0.017659861521230016 14 0.019728133591283982 15 0.016864372263516954 16 0.0224327970675084 17 0.021478209958252722 18 0.01718256796660218 19 0.025932949801445882 20 0.020523622848997045 21 0.018455350778943082 22 0.019728133591283982 23 0.021637307809795336 24 0.01781895937277263 25 0.018773546482028305 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 628544.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.22183291697175 #Duplication Level Percentage of deduplicated Percentage of total 1 93.9283400456247 80.98673641582359 2 4.600746444485178 7.933695824595058 3 0.7367479214885448 1.9057126856553461 4 0.26194478777105984 0.903414388986718 5 0.11652307020544186 0.5023416345113089 6 0.07509887839290812 0.3885097767027182 7 0.048299689122955326 0.2915141407850792 8 0.03669945697853522 0.25314355581974857 9 0.02754564658542037 0.21375325237302617 >10 0.13906504127415023 2.268429345463425 >50 0.014956206618651347 0.8953605049468065 >100 0.01200174663760572 2.1011471698476236 >500 0.001477138047179914 0.8294420937188556 >1k 5.539267676924678E-4 0.5267992107707665 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1291 0.20539532634151308 No Hit CTGTAGGACGTGGAATATGGCAAGA 1012 0.16100702576112413 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1006 0.16005243865186847 No Hit GTCCTACAGTGTGCATTTCTCATTT 957 0.1522566439262804 No Hit CTTTAGGACGTGAAATATGGCGAGG 830 0.1320512167803686 No Hit GTATCAACGCAGAGTACTTTTTTTT 676 0.10755014764280624 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.181957030852255E-4 0.0 10 0.0 0.0 0.0 3.181957030852255E-4 0.0 11 0.0 0.0 0.0 3.181957030852255E-4 0.0 12 0.0 0.0 0.0 3.181957030852255E-4 1.5909785154261276E-4 13 0.0 0.0 0.0 3.181957030852255E-4 1.5909785154261276E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 245 0.0 15.156101 4 GTCGTAG 40 0.005112364 14.317182 1 CGTGGTA 40 0.005156019 14.298852 2 CTAGGGT 60 4.0100547E-4 12.694853 4 TGTAGGA 640 0.0 12.660441 2 CTGTAGG 615 0.0 12.415984 1 AATCCCG 115 5.4515112E-9 12.367478 19 GCCGGTT 100 1.4735633E-7 12.326253 11 TCCAACG 100 1.4735633E-7 12.326253 18 CTACACT 170 0.0 12.321475 4 TAATAGT 70 1.0665307E-4 12.241466 4 TCCTATA 125 1.3278623E-9 12.201687 2 TAGGGTG 55 0.0030197408 12.114909 5 GTAGGAC 600 0.0 12.074587 3 CCAACGA 55 0.0031094428 12.067661 19 CCTACAC 120 9.469659E-9 11.9157095 3 TATACTG 105 2.640827E-7 11.785252 5 CCGGTTT 105 2.7782335E-7 11.739289 12 TTTAAAC 65 7.7848864E-4 11.73239 3 TCTTACA 65 7.7848864E-4 11.73239 2 >>END_MODULE