FastQCFastQC Report
Thu 26 May 2016
SRR1926842_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926842_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628544
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT14270.22703263415130842No Hit
CTGTAGGACGTGGAATATGGCAAGA12380.1969631402097546No Hit
GTATCAACGCAGAGTACTTTTTTTT12120.19282659606964667No Hit
GTCCTAAAGTGTGTATTTCTCATTT9870.1570295794725588No Hit
CTTTAGGACGTGAAATATGGCGAGG9760.15527950310559005No Hit
GTCCTACAGTGTGCATTTCTCATTT9590.15257483962936566No Hit
GGTATCAACGCAGAGTACTTTTTTT7840.12473271560940842No Hit
CTGTAGGACCTGGAATATGGCGAGA7330.11661872518073516No Hit
TATCAACGCAGAGTACTTTTTTTTT6890.1096184197128602No Hit
GTACATGGGAAGCAGTGGTATCAAC6320.10054984217493126No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGA350.002121202416.3392871
TATGTCG350.002185446416.26648116
TAGGACC3000.015.2353894
AGGACTC400.00519369814.2831755
AGACTGT1051.9213985E-812.69615656
TGTAGGA7450.012.6626522
CTGTAGG7000.012.5267861
AGGACCT4900.012.4370525
GGACGTG6000.012.3787536
CCTACAC1001.3942008E-712.3787523
GTAGGAC7500.012.3152723
TAGCACT701.0655118E-412.24272254
GGCCTAG550.00299045312.1306831
CTAGGTT550.00301200912.119064
ACTGTTC2900.012.10541258
CGAAATC550.003094035212.07566913
GGCGAGG2600.012.04345319
GTAGAAC3400.012.0426773
GTATAGG959.961586E-712.0394741
TAGACTG802.7927035E-511.9026475