##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926842_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 628544 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.290554042358213 31.0 30.0 33.0 26.0 34.0 2 30.554314415538133 31.0 30.0 34.0 27.0 34.0 3 30.60588598411567 31.0 31.0 34.0 27.0 34.0 4 34.21529598564301 37.0 35.0 37.0 30.0 37.0 5 34.25376107321047 37.0 35.0 37.0 30.0 37.0 6 34.23460569188474 37.0 35.0 37.0 30.0 37.0 7 34.24943838458405 37.0 35.0 37.0 30.0 37.0 8 34.276540703594335 37.0 35.0 37.0 30.0 37.0 9 35.88205758069443 39.0 37.0 39.0 31.0 39.0 10 35.769024921087464 39.0 37.0 39.0 30.0 39.0 11 35.81866345076876 39.0 37.0 39.0 31.0 39.0 12 35.72174740352306 39.0 37.0 39.0 30.0 39.0 13 35.75342219478668 39.0 37.0 39.0 30.0 39.0 14 36.9078075679666 40.0 37.0 41.0 31.0 41.0 15 36.898031004989306 40.0 37.0 41.0 31.0 41.0 16 36.87603731799206 40.0 37.0 41.0 31.0 41.0 17 36.856156450463295 40.0 37.0 41.0 31.0 41.0 18 36.82955688066389 40.0 37.0 41.0 31.0 41.0 19 36.9025064275532 40.0 37.0 41.0 31.0 41.0 20 36.866430989715916 40.0 37.0 41.0 31.0 41.0 21 36.82659288768964 40.0 37.0 41.0 31.0 41.0 22 36.750596616943284 40.0 37.0 41.0 31.0 41.0 23 36.690500585480095 40.0 37.0 41.0 31.0 41.0 24 36.636292765502496 40.0 37.0 41.0 31.0 41.0 25 36.5250101822625 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18593829556299468 1101 2 0.011825691934927107 1101 3 0.37729756053309416 1101 4 0.3919245627484287 1101 5 0.3416281630275364 1101 6 0.36028725516107585 1101 7 0.2741025404511177 1101 8 0.21705691430391738 1101 9 0.34822602815962256 1101 10 0.31034924939235253 1101 11 0.41447413928379007 1101 12 0.4494173910658219 1101 13 0.3360019701292529 1101 14 0.28534152206334085 1101 15 0.48481639629532935 1101 16 0.35428892294913084 1101 17 0.5043870262713099 1101 18 0.36449258513297167 1101 19 0.3547674310527924 1101 20 0.6120588499638586 1101 21 0.45375027881656393 1101 22 0.1228447273700155 1101 23 0.3183229811964381 1101 24 0.2938888456763067 1101 25 0.2290727984472838 1102 1 -0.7268340597559444 1102 2 -0.7332793065862226 1102 3 -0.5890536176193244 1102 4 -0.6492023227883976 1102 5 -0.7986064815549341 1102 6 -0.6335483950410392 1102 7 -0.49403311961000895 1102 8 -0.34513129431285705 1102 9 -0.5463071474294523 1102 10 -0.5688704094856902 1102 11 -0.5475711135999717 1102 12 -0.5006984523730011 1102 13 -0.47529399753317847 1102 14 -0.7118782354048605 1102 15 -0.7694205158608369 1102 16 -0.6320341570074888 1102 17 -0.6499731235857311 1102 18 -0.7578651985602178 1102 19 -0.7541549784907389 1102 20 -0.7404724524121633 1102 21 -0.6463557056061902 1102 22 -0.7776506881875136 1102 23 -0.9472074338681438 1102 24 -0.9235147824416075 1102 25 -0.9547732037217003 1103 1 -0.33161653321730356 1103 2 -0.37560216493309895 1103 3 -0.20960124670045488 1103 4 -0.18313315175715417 1103 5 -0.30663078272082345 1103 6 -0.17618831236489996 1103 7 -0.34006637066854495 1103 8 -0.12165989985537351 1103 9 -0.29452118230593527 1103 10 -0.17013362863765735 1103 11 -0.09642428318639418 1103 12 -0.15444948080797616 1103 13 -0.16286297792769489 1103 14 -0.06278275650533516 1103 15 -0.11875228396630888 1103 16 -0.03954524403816606 1103 17 0.0873420089569592 1103 18 -0.11986678385510174 1103 19 -0.27096361935121394 1103 20 -0.2322274139838143 1103 21 -0.0749145922877048 1103 22 -0.0902372272279095 1103 23 -0.26519952746073727 1103 24 -0.24925274724405 1103 25 0.06569265223720322 1104 1 -0.0915916076076897 1104 2 -0.15499602820936786 1104 3 0.1751806059099721 1104 4 0.07127713537040847 1104 5 0.10171929650010725 1104 6 -0.016070253857343175 1104 7 -0.030539570807661676 1104 8 -0.13043216866551433 1104 9 -0.14441684254475717 1104 10 -0.00825242138797222 1104 11 -0.1010422837274163 1104 12 0.22837600128036684 1104 13 0.1146662951719648 1104 14 0.033784658573686954 1104 15 -0.021358845777960767 1104 16 -0.01607845886214676 1104 17 -0.005167748742927358 1104 18 0.049574225535479854 1104 19 0.06310234656446312 1104 20 0.15211485907720856 1104 21 0.010542570443675459 1104 22 0.11885289141026334 1104 23 0.1282289998028503 1104 24 0.029150854409934368 1104 25 -0.0658768882814087 1105 1 0.17515791652174784 1105 2 0.1183585793373787 1105 3 0.2531058102543895 1105 4 0.4663392784674869 1105 5 0.3212714183229721 1105 6 0.0915414357630766 1105 7 -0.1749052633392978 1105 8 -0.24281488056787737 1105 9 0.11626393228448961 1105 10 0.12038826834442062 1105 11 0.04952988064387398 1105 12 0.2963571903969253 1105 13 0.29648691996202814 1105 14 0.4341709535014715 1105 15 0.2156134977446058 1105 16 0.3007270500394341 1105 17 0.21499460709628693 1105 18 0.48207898424111306 1105 19 0.6024820352891354 1105 20 0.4384635322381101 1105 21 0.3429308808232534 1105 22 0.4449238801013422 1105 23 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1107 10 0.3380831765464265 1107 11 0.249175171120811 1107 12 0.19010660528224577 1107 13 0.08830155563061481 1107 14 0.20474358573738982 1107 15 0.12764865663574199 1107 16 0.20695891148427847 1107 17 0.3171769548364409 1107 18 0.2581803000990206 1107 19 0.10550382756041898 1107 20 0.10099746888969463 1107 21 0.12326478626872017 1107 22 0.275818975540048 1107 23 0.10339441606723909 1107 24 0.1683817462848225 1107 25 0.21930562321136904 1108 1 0.34474859629498056 1108 2 0.27028763743276585 1108 3 0.5652241854559286 1108 4 0.5128832010763205 1108 5 0.47345636657282597 1108 6 0.43003105972937306 1108 7 0.30844419315418037 1108 8 0.31741188387795916 1108 9 0.4017801334599156 1108 10 0.5104969696498713 1108 11 0.42158896422425585 1108 12 0.4091735424019163 1108 13 0.17045165705049214 1108 14 0.3112344579483377 1108 15 0.39438293655460654 1108 16 0.3225775727418849 1108 17 -0.006352456928262029 1108 18 0.32004642585966536 1108 19 0.3732523062622448 1108 20 0.4397398623988238 1108 21 0.4528793907311979 1108 22 0.3660826672236155 1108 23 0.40461145460678694 1108 24 0.18156633046332615 1108 25 0.4018614041444337 1109 1 0.527711271350551 1109 2 0.2853568904075452 1109 3 0.4999045205261545 1109 4 0.39703173969887473 1109 5 0.3815570010037632 1109 6 0.4897695948295606 1109 7 0.3739723660173766 1109 8 0.45273555701849233 1109 9 0.2981739583861298 1109 10 0.3719282652736382 1109 11 0.2786132523619784 1109 12 0.23206730862201397 1109 13 0.27985556728276606 1109 14 0.46022653464687124 1109 15 0.23078315842324315 1109 16 0.19237145354012597 1109 17 0.5567019616180104 1109 18 0.37351150381734044 1109 19 0.3875887449467399 1109 20 0.357895437658037 1109 21 0.4977166938126558 1109 22 0.4626238647474352 1109 23 0.37579282411928716 1109 24 0.46479702930903954 1109 25 0.6083794026302201 1110 1 0.12748124360117075 1110 2 -0.03701665518419617 1110 3 0.16817773020435922 1110 4 0.2622586030453533 1110 5 0.2177504677288482 1110 6 0.2148560073703365 1110 7 0.1082842300940996 1110 8 0.1975447191819697 1110 9 0.13702065523633422 1110 10 0.3097660044294699 1110 11 0.1679382608815274 1110 12 0.26505012348010126 1110 13 0.1570341369726549 1110 14 0.1459283018441866 1110 15 0.04122325384216197 1110 16 0.01394781301629422 1110 17 0.12332481347527136 1110 18 0.054983924828974295 1110 19 0.24973031546424096 1110 20 0.20346040528376363 1110 21 0.05400655311844105 1110 22 0.06034914589245943 1110 23 0.007365045530121961 1110 24 0.2634010407982572 1110 25 0.24714455525180767 1111 1 0.5010901417504527 1111 2 0.4660096552296622 1111 3 0.47037162651518116 1111 4 0.5106376936201755 1111 5 0.6139244269558759 1111 6 0.624297523896054 1111 7 0.5208143604205304 1111 8 0.33778555752000017 1111 9 0.35841829383583246 1111 10 0.47917708421866223 1111 11 0.5302978229193585 1111 12 0.48472982278966015 1111 13 0.6045348519295572 1111 14 0.4936220551636268 1111 15 0.5991980873593405 1111 16 0.29806759893777013 1111 17 0.44670186131610023 1111 18 0.4750180384866951 1111 19 0.5752175075220904 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0.22676798748150873 2214 6 0.06094555277282865 2214 7 -0.022243652649514445 2214 8 -0.01075998710452808 2214 9 -0.07129836457358607 2214 10 -0.06977424519838138 2214 11 0.011162238895956023 2214 12 0.0033416613250594196 2214 13 -0.005123769817679147 2214 14 7.922363509038632E-5 2214 15 -0.09048978864037593 2214 16 -0.1395618629255324 2214 17 -0.20944742974011632 2214 18 -0.04901527361371194 2214 19 0.03715159108544697 2214 20 -0.17320855154665082 2214 21 0.07437183423603955 2214 22 0.15551976309158988 2214 23 0.08975082361325804 2214 24 -0.15482007451148405 2214 25 -0.024866781058740628 2215 1 0.36325176360293554 2215 2 0.41155837532198447 2215 3 0.4039427370406834 2215 4 0.5299503509393233 2215 5 0.5221690860560813 2215 6 0.4200266214634567 2215 7 0.40287838760891503 2215 8 0.48554696855347146 2215 9 0.1797182838401028 2215 10 0.32799804253647125 2215 11 0.2123100540569638 2215 12 0.3187998110673931 2215 13 0.4373326565039619 2215 14 0.24855403877062798 2215 15 0.48438986905262027 2215 16 0.2552186344044358 2215 17 0.1671140488359839 2215 18 0.06804828919775474 2215 19 0.1388385058228181 2215 20 0.18561612647931014 2215 21 0.12417444738327532 2215 22 0.12693899578734147 2215 23 0.20789173660173788 2215 24 0.12413296864195189 2215 25 0.28114057107091384 2216 1 -0.08797863684736384 2216 2 0.14467939030935284 2216 3 -1.9582239452614125 2216 4 -1.4177394335182 2216 5 -0.6038567868052382 2216 6 -0.11976446763584647 2216 7 -0.3693834982826445 2216 8 -0.13638673506996213 2216 9 -0.12635980057180518 2216 10 -0.17709533332523364 2216 11 -0.2464868158379332 2216 12 -0.0622474607658674 2216 13 -0.27732345570881733 2216 14 -0.2534855760155281 2216 15 -0.25892436749093406 2216 16 -0.1240672254525137 2216 17 -0.27894695521737134 2216 18 -0.26383634074398543 2216 19 -0.49435755922488056 2216 20 -0.33133968779767287 2216 21 -0.32851813527692286 2216 22 -0.33556508829460796 2216 23 -0.24228408110425903 2216 24 -0.38969791639883056 2216 25 -0.5400607474810073 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11788.0 3 9.0 4 197.0 5 1113.0 6 1529.0 7 1118.0 8 929.0 9 820.0 10 662.0 11 679.0 12 587.0 13 628.0 14 567.0 15 579.0 16 620.0 17 698.0 18 795.0 19 850.0 20 1133.0 21 1508.0 22 1903.0 23 2403.0 24 2846.0 25 3481.0 26 4152.0 27 4647.0 28 5524.0 29 7016.0 30 8840.0 31 11810.0 32 15559.0 33 20844.0 34 29072.0 35 41427.0 36 62536.0 37 110552.0 38 269123.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.933721545116875 19.356965831841435 10.993316726023995 27.71599589701769 2 14.04881405391113 20.72550496885209 40.82311396264162 24.402567014595157 3 18.79036787977831 24.393457439783337 29.360367513792113 27.455807166646245 4 12.020001781895937 17.412941655635883 38.00704485286631 32.56001170960187 5 12.912305153943699 38.53182691099094 35.5485760744874 13.007291860577972 6 31.78723064644852 37.994573024740625 17.766959297685556 12.451237031125299 7 28.05419495288018 31.681195675352246 21.50691122505164 18.757698146715935 8 24.725049166555284 38.668620599672856 19.66200777754441 16.944322456227447 9 25.913408754594492 15.952710551020733 19.88257196047544 38.25130873390933 10 14.64545055336698 30.070833518955766 34.04941162993464 21.234304297742618 11 34.46128487193603 22.7786493220517 24.189437307681295 18.570628498330986 12 23.14618003627454 25.013841601170967 31.80624940337926 20.03372895917523 13 29.141707992662347 22.138323954440377 25.65950561540334 23.060462437493932 14 20.464471006756895 22.792394474204627 27.325417672299267 29.417716846739207 15 22.681161956705054 31.629717784148948 23.101669620668037 22.587450638477964 16 21.70935819518249 28.803417443599454 28.225252171699495 21.261972189518566 17 21.324396262467324 29.12720370762223 27.94017274872204 21.608227281188398 18 21.01823272981831 28.360327107264453 29.988863079517614 20.632577083399624 19 23.282771875228704 27.086495413194346 27.42060196454652 22.21013074703043 20 22.99332261005284 28.16881689123507 28.11313220022942 20.724728298482674 21 22.490775230678903 27.188207570436823 27.220189953076595 23.100827245807682 22 22.55021128194685 28.70936640871602 27.282576876081865 21.45784543325527 23 22.12122995954122 28.20866101018072 27.65388415393745 22.016224876340605 24 22.775334741879647 27.373739945015785 28.462923836676506 21.388001476428062 25 22.469491336653356 27.74243846557732 28.05985584954893 21.728214348220394 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 146.0 1 146.0 2 151.0 3 156.0 4 156.0 5 156.0 6 313.0 7 470.0 8 470.0 9 470.0 10 763.5 11 1057.0 12 1057.0 13 1057.0 14 1841.0 15 2625.0 16 2625.0 17 2625.0 18 4707.0 19 6789.0 20 6789.0 21 6789.0 22 10965.0 23 15141.0 24 15141.0 25 15141.0 26 22248.5 27 29356.0 28 29356.0 29 29356.0 30 39282.5 31 49209.0 32 49209.0 33 49209.0 34 55696.5 35 62184.0 36 62184.0 37 62184.0 38 66907.0 39 71630.0 40 71630.0 41 71630.0 42 75526.5 43 79423.0 44 79423.0 45 79423.0 46 82041.5 47 84660.0 48 84660.0 49 84660.0 50 80979.0 51 77298.0 52 77298.0 53 77298.0 54 67918.5 55 58539.0 56 58539.0 57 58539.0 58 49748.0 59 40957.0 60 40957.0 61 40957.0 62 33225.5 63 25494.0 64 25494.0 65 25494.0 66 19628.0 67 13762.0 68 13762.0 69 13762.0 70 9929.5 71 6097.0 72 6097.0 73 6097.0 74 4244.0 75 2391.0 76 2391.0 77 2391.0 78 1615.0 79 839.0 80 839.0 81 839.0 82 542.0 83 245.0 84 245.0 85 245.0 86 152.0 87 59.0 88 59.0 89 59.0 90 37.0 91 15.0 92 15.0 93 15.0 94 8.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11264127889216984 2 0.11661872518073516 3 0.016705274411974343 4 0.0 5 0.005568424803991447 6 0.3251960085531005 7 0.023228286325221465 8 0.010818653904897668 9 0.011773241014153345 10 0.004454739843193158 11 0.0047729355462783835 12 6.36391406170451E-4 13 7.954892577130639E-4 14 1.5909785154261276E-4 15 0.00286376132776703 16 6.36391406170451E-4 17 1.5909785154261276E-4 18 6.36391406170451E-4 19 3.181957030852255E-4 20 7.954892577130639E-4 21 0.011614143162610733 22 0.0 23 4.772935546278383E-4 24 0.0 25 0.005409326952448834 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 628544.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.54448650773965 #Duplication Level Percentage of deduplicated Percentage of total 1 92.25777513910117 77.07628450342716 2 5.959465243788128 9.957609273060013 3 0.9315233306704007 2.3347091499251396 4 0.30173982294734214 1.008347942682879 5 0.150831134747201 0.6300554850917297 6 0.09074297971364062 0.4548645386615005 7 0.05384317655331751 0.31488103759647346 8 0.037378233655536636 0.24981962698544963 9 0.022471111753268908 0.16896037434163955 >10 0.15950707573236889 2.4907535282662914 >50 0.018124614097742905 1.0543667391099643 >100 0.01392717262709234 2.3790377307588 >500 0.0020986150528840585 1.2623595621884032 >1k 5.723495598774704E-4 0.6179505079046008 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1427 0.22703263415130842 No Hit CTGTAGGACGTGGAATATGGCAAGA 1238 0.1969631402097546 No Hit GTATCAACGCAGAGTACTTTTTTTT 1212 0.19282659606964667 No Hit GTCCTAAAGTGTGTATTTCTCATTT 987 0.1570295794725588 No Hit CTTTAGGACGTGAAATATGGCGAGG 976 0.15527950310559005 No Hit GTCCTACAGTGTGCATTTCTCATTT 959 0.15257483962936566 No Hit GGTATCAACGCAGAGTACTTTTTTT 784 0.12473271560940842 No Hit CTGTAGGACCTGGAATATGGCGAGA 733 0.11661872518073516 No Hit TATCAACGCAGAGTACTTTTTTTTT 689 0.1096184197128602 No Hit GTACATGGGAAGCAGTGGTATCAAC 632 0.10054984217493126 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.5909785154261276E-4 0.0 10 0.0 0.0 0.0 1.5909785154261276E-4 0.0 11 0.0 0.0 0.0 1.5909785154261276E-4 0.0 12 0.0 0.0 0.0 1.5909785154261276E-4 1.5909785154261276E-4 13 0.0 0.0 0.0 1.5909785154261276E-4 1.5909785154261276E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTGA 35 0.0021212024 16.339287 1 TATGTCG 35 0.0021854464 16.266481 16 TAGGACC 300 0.0 15.235389 4 AGGACTC 40 0.005193698 14.283175 5 AGACTGT 105 1.9213985E-8 12.6961565 6 TGTAGGA 745 0.0 12.662652 2 CTGTAGG 700 0.0 12.526786 1 AGGACCT 490 0.0 12.437052 5 GGACGTG 600 0.0 12.378753 6 CCTACAC 100 1.3942008E-7 12.378752 3 GTAGGAC 750 0.0 12.315272 3 TAGCACT 70 1.0655118E-4 12.2427225 4 GGCCTAG 55 0.002990453 12.130683 1 CTAGGTT 55 0.003012009 12.11906 4 ACTGTTC 290 0.0 12.1054125 8 CGAAATC 55 0.0030940352 12.075669 13 GGCGAGG 260 0.0 12.043453 19 GTAGAAC 340 0.0 12.042677 3 GTATAGG 95 9.961586E-7 12.039474 1 TAGACTG 80 2.7927035E-5 11.902647 5 >>END_MODULE