FastQCFastQC Report
Thu 26 May 2016
SRR1926841_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926841_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659253
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12720.19294565212444995No Hit
CTGTAGGACGTGGAATATGGCAAGA11300.1714061217772236No Hit
GTCCTAAAGTGTGTATTTCTCATTT9260.14046200775726464No Hit
GTCCTACAGTGTGCATTTCTCATTT9080.13773164475550356No Hit
CTTTAGGACGTGAAATATGGCGAGG8070.12241127457895529No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAGCG400.00515581914.2990852
TAGGACC2400.013.4913134
AACTGGT604.0209744E-412.6909377
CCAACGA905.51383E-712.64279219
CTAGGAC1001.3745012E-712.3925393
AAACAGT701.0697626E-412.2376896
TCCAACG1103.8995495E-812.0681218
GGCGAGG2750.012.0681219
TAGGACA2300.012.0076144
GTCCTAC7800.011.8620351
TACTAGG657.7847176E-411.7325822
AGTGCAA658.124944E-411.67292710
GTCCTAG1251.7173079E-811.4462521
CCCGTTT1001.9679974E-611.37851212
GTAGGAC6550.011.3519463
TGTAGGA7400.011.3362112
GTATAAA1104.6983223E-711.2728241
CGTTTCC855.4148994E-511.15540514
GTTCTAT600.00569523411.1283021
GTACTAA600.00569523411.1283021
Produced by FastQC (version 0.11.5)