##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926841_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 659253 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.333949181877063 31.0 28.0 31.0 20.0 34.0 2 28.505473619384365 31.0 29.0 33.0 22.0 34.0 3 28.11091948007821 31.0 28.0 31.0 16.0 34.0 4 31.716304666038685 35.0 32.0 37.0 19.0 37.0 5 31.86864071911694 35.0 32.0 37.0 19.0 37.0 6 31.041067693283157 35.0 32.0 37.0 16.0 37.0 7 31.06570921937405 35.0 32.0 37.0 16.0 37.0 8 30.983027760207385 35.0 32.0 37.0 11.0 37.0 9 33.13867513685945 37.0 33.0 39.0 17.0 39.0 10 33.37553716099889 37.0 34.0 39.0 17.0 39.0 11 33.54595580149047 38.0 34.0 39.0 19.0 39.0 12 33.437329826333745 38.0 34.0 39.0 18.0 39.0 13 33.56208466248921 38.0 34.0 39.0 19.0 39.0 14 34.606043506817564 39.0 34.0 41.0 20.0 41.0 15 34.171864216014185 39.0 34.0 41.0 17.0 41.0 16 34.259856231219274 39.0 34.0 41.0 17.0 41.0 17 34.493699687373436 39.0 34.0 41.0 18.0 41.0 18 34.543926231659164 39.0 34.0 41.0 18.0 41.0 19 33.89349157303797 39.0 33.0 41.0 10.0 41.0 20 34.17970187469757 39.0 34.0 41.0 17.0 41.0 21 34.17468862485267 39.0 34.0 41.0 16.0 41.0 22 34.202869004767514 39.0 34.0 41.0 11.0 41.0 23 34.21092054188605 39.0 34.0 41.0 10.0 41.0 24 32.89126632719153 39.0 32.0 40.0 2.0 41.0 25 33.4805150678116 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.6863280681699955 1101 2 -0.6849816741877355 1101 3 -1.7987008508506293 1101 4 -1.285338622575594 1101 5 -1.3193046179416612 1101 6 -2.918007280060749 1101 7 -1.2870945128850266 1101 8 -0.9136872375826215 1101 9 -0.9859771090040326 1101 10 -9.886255371093679 1101 11 -4.645780568411919 1101 12 -2.5105135972804398 1101 13 -1.6286588617033075 1101 14 -1.3854435275602555 1101 15 -2.9324988707291304 1101 16 -1.238975450827013 1101 17 -0.953638495980428 1101 18 -0.9578221407255967 1101 19 -1.5485647857722356 1101 20 -1.1815528657247114 1101 21 -1.0820439631984016 1101 22 -0.9285842809974127 1101 23 -1.0118128329984444 1101 24 -1.220161916333673 1101 25 -1.030511849712859 1102 1 0.10077494053252423 1102 2 -0.1454071044443559 1102 3 -0.3895510979798331 1102 4 -0.3160250423605646 1102 5 -0.12053052513127582 1102 6 -0.895548815307194 1102 7 -0.06729103252246205 1102 8 0.5961446629071183 1102 9 0.48305937228075635 1102 10 0.4633197259447499 1102 11 0.30870310750178476 1102 12 0.41000044006711533 1102 13 0.16982080676799427 1102 14 -0.01549977595281149 1102 15 0.535786484135059 1102 16 0.6364322612397615 1102 17 0.35189455089179944 1102 18 0.2248059623788734 1102 19 0.4952674224856395 1102 20 0.510524041872749 1102 21 0.581772081851966 1102 22 0.5775630108044467 1102 23 -0.018927011747159384 1102 24 -3.3593893322328015 1102 25 -1.4437379161671018 1103 1 0.0341445128769422 1103 2 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2216 12 -2.28837688666146 2216 13 -2.2181709773644194 2216 14 -2.2018016696629843 2216 15 -2.998153249085785 2216 16 -1.8652442251758714 2216 17 -2.1497208290230887 2216 18 -2.010420555477033 2216 19 -1.4341151456037622 2216 20 -2.281028871428955 2216 21 -4.962229526628828 2216 22 -3.5923061621486383 2216 23 -3.308958176062543 2216 24 -3.548782032174259 2216 25 -2.935024326278455 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36480.0 3 5.0 4 273.0 5 1673.0 6 2251.0 7 1824.0 8 1433.0 9 1239.0 10 1134.0 11 1143.0 12 1142.0 13 1127.0 14 1151.0 15 1145.0 16 1247.0 17 1404.0 18 1536.0 19 1957.0 20 2463.0 21 3384.0 22 4141.0 23 5050.0 24 5898.0 25 6945.0 26 7729.0 27 8649.0 28 9684.0 29 11451.0 30 13827.0 31 17029.0 32 21976.0 33 28945.0 34 39157.0 35 53831.0 36 77555.0 37 121797.0 38 161578.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.31703372453408 18.46056097609171 12.759295261054676 25.463110038319535 2 14.10068118132181 19.655992041493587 42.72426964756271 23.519057129621896 3 18.905442411410643 24.107879216832245 29.893241677209854 27.09343669454726 4 11.794286442862152 16.992679324670355 38.513764782132874 32.69926945033461 5 12.682766227638304 37.64108103843534 36.785529511900194 12.890623222026164 6 31.515328981006235 36.929743245067975 18.51903548871131 13.035892285214478 7 27.712602833081828 31.924043469795066 22.18684588424281 18.1765078128803 8 24.38279398477026 38.41097782469676 20.642441869309003 16.56378632122398 9 26.05103339669902 15.855884285057584 20.154231973019265 37.93885034522413 10 14.497565327963752 29.636081399530518 34.6429021229976 21.223451149508136 11 34.21370823984186 22.727417541655857 24.545968981310807 18.51290523719148 12 23.155591899153595 24.651478211793833 32.01060167703116 20.182328212021403 13 28.927358842644708 22.13293322693497 25.606152091000943 23.333555839419382 14 20.476495343975387 22.450413110297262 27.585694216455835 29.487397329271513 15 22.988535391309227 31.44946818597637 22.955462945965994 22.606533476748403 16 21.664631139768634 28.49125734875782 28.35714014792338 21.48697136355016 17 21.569243233156282 28.89507883841349 27.883750320496777 21.65192760793345 18 21.335975083401728 28.060756206014027 29.750333379351567 20.85293533123268 19 23.84230952460424 27.095464517518973 26.922353341000193 22.139872616876595 20 23.325626344550944 27.882004181193125 27.913408643357577 20.87896083089835 21 22.728169185023365 27.14940226955519 26.900145639905332 23.22228290551611 22 22.68702049188411 28.42602243229425 27.302435449882346 21.584521625939292 23 22.10751011169133 28.192123652406625 27.540059896410156 22.160306339491882 24 23.303193506789047 27.268755215049683 27.990138815140714 21.437912463020556 25 22.615655825497534 27.594461992189988 27.8960607081198 21.893821474192677 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 211.0 1 211.0 2 251.0 3 291.0 4 291.0 5 291.0 6 411.0 7 531.0 8 531.0 9 531.0 10 799.5 11 1068.0 12 1068.0 13 1068.0 14 1843.0 15 2618.0 16 2618.0 17 2618.0 18 4803.5 19 6989.0 20 6989.0 21 6989.0 22 11266.0 23 15543.0 24 15543.0 25 15543.0 26 23065.5 27 30588.0 28 30588.0 29 30588.0 30 40796.5 31 51005.0 32 51005.0 33 51005.0 34 58270.0 35 65535.0 36 65535.0 37 65535.0 38 71080.0 39 76625.0 40 76625.0 41 76625.0 42 80613.5 43 84602.0 44 84602.0 45 84602.0 46 86511.0 47 88420.0 48 88420.0 49 88420.0 50 84181.5 51 79943.0 52 79943.0 53 79943.0 54 70638.0 55 61333.0 56 61333.0 57 61333.0 58 52210.5 59 43088.0 60 43088.0 61 43088.0 62 34886.5 63 26685.0 64 26685.0 65 26685.0 66 20481.5 67 14278.0 68 14278.0 69 14278.0 70 10287.0 71 6296.0 72 6296.0 73 6296.0 74 4337.5 75 2379.0 76 2379.0 77 2379.0 78 1613.5 79 848.0 80 848.0 81 848.0 82 551.5 83 255.0 84 255.0 85 255.0 86 176.5 87 98.0 88 98.0 89 98.0 90 56.5 91 15.0 92 15.0 93 15.0 94 9.0 95 3.0 96 3.0 97 3.0 98 4.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2069008408001177 2 0.12711356641532157 3 0.12893380841649563 4 0.06583208570912835 5 0.022146277680951017 6 0.02153953034722633 7 0.28471618635030865 8 0.2813790760148228 9 0.017595672678015876 10 0.03519134535603175 11 0.015472057009979478 12 0.016988925344291188 13 0.014410249175961279 14 0.016230491177135334 15 0.014258562342530106 16 0.01941591467918993 17 0.01820242001174056 18 0.014258562342530106 19 0.021084469846932817 20 0.01683723851086002 21 0.014106875509098935 22 0.01729229901115353 23 0.017443985844584703 24 0.015623743843410648 25 0.015016996509685963 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 659253.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.99928311645873 #Duplication Level Percentage of deduplicated Percentage of total 1 93.56146359375 80.46218796389152 2 4.829463371597481 8.306607755891582 3 0.7952742408451243 2.051790437809999 4 0.29222302124971 1.00523881350403 5 0.14304705362502368 0.6150972031836832 6 0.0877212481723748 0.45263786741431183 7 0.04944695987256783 0.2976682170930394 8 0.04014109700109265 0.27616844524817585 9 0.025378401767139565 0.19642719227539246 >10 0.14881777964328044 2.3986635020824574 >50 0.014129969931619274 0.8329927541590373 >100 0.011480302266384992 2.0599588128290938 >500 0.001059720208581796 0.6797165377220201 >1k 3.5324006952726535E-4 0.3648444968957078 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1272 0.19294565212444995 No Hit CTGTAGGACGTGGAATATGGCAAGA 1130 0.1714061217772236 No Hit GTCCTAAAGTGTGTATTTCTCATTT 926 0.14046200775726464 No Hit GTCCTACAGTGTGCATTTCTCATTT 908 0.13773164475550356 No Hit CTTTAGGACGTGAAATATGGCGAGG 807 0.12241127457895529 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAGCG 40 0.005155819 14.299085 2 TAGGACC 240 0.0 13.491313 4 AACTGGT 60 4.0209744E-4 12.690937 7 CCAACGA 90 5.51383E-7 12.642792 19 CTAGGAC 100 1.3745012E-7 12.392539 3 AAACAGT 70 1.0697626E-4 12.237689 6 TCCAACG 110 3.8995495E-8 12.06812 18 GGCGAGG 275 0.0 12.06812 19 TAGGACA 230 0.0 12.007614 4 GTCCTAC 780 0.0 11.862035 1 TACTAGG 65 7.7847176E-4 11.732582 2 AGTGCAA 65 8.124944E-4 11.672927 10 GTCCTAG 125 1.7173079E-8 11.446252 1 CCCGTTT 100 1.9679974E-6 11.378512 12 GTAGGAC 655 0.0 11.351946 3 TGTAGGA 740 0.0 11.336211 2 GTATAAA 110 4.6983223E-7 11.272824 1 CGTTTCC 85 5.4148994E-5 11.155405 14 GTTCTAT 60 0.005695234 11.128302 1 GTACTAA 60 0.005695234 11.128302 1 >>END_MODULE