FastQCFastQC Report
Thu 26 May 2016
SRR1926841_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926841_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659253
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12760.19355239945817462No Hit
CTGTAGGACGTGGAATATGGCAAGA12400.18809167345465247No Hit
GTATCAACGCAGAGTACTTTTTTTT12210.1852096236194602No Hit
CTTTAGGACGTGAAATATGGCGAGG9860.14956321776313494No Hit
GTCCTAAAGTGTGTATTTCTCATTT9680.14683285476137384No Hit
GTCCTACAGTGTGCATTTCTCATTT9410.14273731025873224No Hit
GGTATCAACGCAGAGTACTTTTTTT8200.1243832034135605No Hit
CTGTAGGACCTGGAATATGGCGAGA7450.11300669090622265No Hit
TATCAACGCAGAGTACTTTTTTTTT7170.10875945957014985No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAG351.00203215E-418.97724318
ATGTCGA402.7834196E-416.60508717
GTCGAGG350.002185844116.26620919
TATGTCG350.002185844116.26620916
GTTCCAA456.5810827E-414.8286921
GTCCTAT954.651156E-914.0482361
TTAGAAC707.0662336E-613.604713
TCTGAAC500.001470545713.3326153
TCCTATA1153.5470293E-1013.2629312
GGATAGA550.002987241812.1325671
GTCCTAC6950.011.7958981
GACGTGA3300.011.7888957
TAGGCCT907.241948E-611.6395844
TAGGACC3600.011.6395844
AGGACGT6800.011.6240235
GGACGTG6650.011.5998056
TTTAGGA4200.011.5754592
TAGGACG6950.011.5101724
GTTTAGA752.0052382E-411.4392781
TTAGGAC4200.011.3372583
Produced by FastQC (version 0.11.5)