##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926841_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 659253 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.176461843935485 31.0 30.0 33.0 26.0 34.0 2 30.450244443332075 31.0 30.0 34.0 27.0 34.0 3 30.506080366718088 31.0 31.0 34.0 27.0 34.0 4 34.13149579903315 37.0 35.0 37.0 30.0 37.0 5 34.196616473493485 37.0 35.0 37.0 30.0 37.0 6 34.17708982742589 37.0 35.0 37.0 30.0 37.0 7 34.18577238177149 37.0 35.0 37.0 30.0 37.0 8 34.21909342847132 37.0 35.0 37.0 30.0 37.0 9 35.81805316016764 39.0 37.0 39.0 31.0 39.0 10 35.70497062584471 39.0 37.0 39.0 30.0 39.0 11 35.76278909614366 39.0 37.0 39.0 30.0 39.0 12 35.674721237521865 39.0 37.0 39.0 30.0 39.0 13 35.695423456548546 39.0 37.0 39.0 30.0 39.0 14 36.835292368787094 40.0 37.0 41.0 31.0 41.0 15 36.819689861100365 40.0 37.0 41.0 31.0 41.0 16 36.80392049789686 40.0 37.0 41.0 31.0 41.0 17 36.784189074604136 40.0 37.0 41.0 31.0 41.0 18 36.75371215603114 40.0 37.0 41.0 31.0 41.0 19 36.825880200772694 40.0 37.0 41.0 31.0 41.0 20 36.78602751902532 40.0 37.0 41.0 31.0 41.0 21 36.73868302457478 40.0 37.0 41.0 31.0 41.0 22 36.66113009724643 40.0 37.0 41.0 30.0 41.0 23 36.599018889561364 40.0 37.0 41.0 30.0 41.0 24 36.541829919621144 40.0 37.0 41.0 30.0 41.0 25 36.427430743584026 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3300753483690322 1101 2 0.3968637288751111 1101 3 0.602988718911174 1101 4 0.5673702189681791 1101 5 0.49974571065685325 1101 6 0.4424184126096975 1101 7 0.7050078519354628 1101 8 0.5827098242258657 1101 9 0.8129165386162072 1101 10 0.6976355872908684 1101 11 0.6501992719613696 1101 12 0.815786042725108 1101 13 0.7739183856292726 1101 14 0.8085487307159553 1101 15 0.7857305209235079 1101 16 0.9081431691090032 1101 17 0.6486723632746987 1101 18 0.9146133501478886 1101 19 0.8854093233977238 1101 20 0.8383057240544929 1101 21 0.8350759960840364 1101 22 0.9490808614715931 1101 23 0.8690252028236358 1101 24 1.0241284221230629 1101 25 0.7530817587861023 1102 1 -0.19539138846015902 1102 2 -0.39097534067510864 1102 3 -0.02561800614535059 1102 4 -0.21482679352871514 1102 5 -0.2425684259082601 1102 6 -0.2903075629130498 1102 7 -0.09171022061818235 1102 8 -0.1814687630753653 1102 9 -0.07717903092163425 1102 10 0.015797888707780317 1102 11 -0.022264411937079842 1102 12 -0.09648820235517519 1102 13 -0.10113958992360494 1102 14 -0.1013591433057286 1102 15 -0.15986769531265566 1102 16 -0.11990677541740524 1102 17 -0.17585030018945247 1102 18 -0.08800267897601088 1102 19 -0.07703327945978344 1102 20 -0.01224056438020682 1102 21 -0.07867037069163985 1102 22 -0.04115126672351721 1102 23 -0.12717504446698058 1102 24 -0.026423031650914197 1102 25 -0.021993542175621883 1103 1 -0.2162041434639974 1103 2 -0.3114384667762913 1103 3 -0.1140320276814748 1103 4 -0.11385108988203996 1103 5 -0.16770384629841573 1103 6 -0.21248710676683658 1103 7 -0.3256316270866364 1103 8 -0.39483545886014326 1103 9 -0.32590414392178246 1103 10 -0.15429139039675732 1103 11 -0.20982350302884356 1103 12 -0.2305824139134458 1103 13 -0.06704812801792315 1103 14 -0.08990684026016993 1103 15 -0.2451936454762631 1103 16 -0.1598793211938343 1103 17 -0.12544599550256663 1103 18 -0.2811626008858781 1103 19 -0.13886027554892877 1103 20 -0.057996898627976634 1103 21 0.04732822910064982 1103 22 -0.030457233258132987 1103 23 0.19773236626978274 1103 24 -0.027987160952271495 1103 25 -0.050056919552744716 1104 1 -0.38354694265263944 1104 2 -0.3002822801637137 1104 3 -0.09374805202224579 1104 4 -0.18687340225526583 1104 5 -0.051070986257848006 1104 6 -0.1769901493631849 1104 7 -0.05889734716777184 1104 8 -0.011468318900710983 1104 9 -0.03888588834369244 1104 10 -0.11676603542718311 1104 11 -0.17229814805926935 1104 12 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23 0.463393858232962 1105 24 0.2648415243066893 1105 25 0.24216567081600004 1106 1 0.32824092209956035 1106 2 0.054567506614965566 1106 3 0.42535002978446457 1106 4 0.37711096151468126 1106 5 0.2896399111882886 1106 6 0.473241040044428 1106 7 0.26105841140500274 1106 8 0.33928674011447413 1106 9 0.4407981927283302 1106 10 0.32228820424315785 1106 11 0.3861201011301745 1106 12 0.13556331127861654 1106 13 0.21619083957563134 1106 14 0.25631227459185624 1106 15 0.3310010327751627 1106 16 0.32411710116006276 1106 17 0.20858476586524688 1106 18 0.1788763699019995 1106 19 0.052749425621790635 1106 20 0.2627813991152337 1106 21 0.15606910530451756 1106 22 0.1597829002647302 1106 23 0.28455017783643655 1106 24 0.47398134298473593 1106 25 0.40465351471598865 1107 1 0.46995445790758694 1107 2 0.3534876415980932 1107 3 0.5235165089745664 1107 4 0.48292086027733205 1107 5 0.44767140725127774 1107 6 0.5565537509555654 1107 7 0.4855091050682958 1107 8 0.37981729879464154 1107 9 0.2607007049143064 1107 10 0.3839661195037536 1107 11 0.34708560544213185 1107 12 0.32574328878142467 1107 13 0.22912109829329097 1107 14 0.23538051606167443 1107 15 0.2962185055910638 1107 16 0.4247995158582256 1107 17 0.41753864663014895 1107 18 0.2654905431303476 1107 19 0.4122263660042407 1107 20 0.3056947849420055 1107 21 0.38335256987144106 1107 22 0.3180562410971248 1107 23 0.1732003465653449 1107 24 0.29296522000011294 1107 25 0.25518407339615834 1108 1 0.43837314613759304 1108 2 0.3223286119484996 1108 3 0.4788264820203878 1108 4 0.565912774023964 1108 5 0.3461619732890142 1108 6 0.4580092765620378 1108 7 0.43762950039624116 1108 8 0.42781370490425275 1108 9 0.35704392144619845 1108 10 0.45285201348398374 1108 11 0.28946655782308284 1108 12 0.35760312705635755 1108 13 0.31178956190515095 1108 14 0.49512894373462757 1108 15 0.3969013447644656 1108 16 0.39058567938023714 1108 17 0.5420939026948375 1108 18 0.47586790162091575 1108 19 0.3185605978101691 1108 20 0.5811664830552132 1108 21 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2210 3 0.19614109369217303 2210 4 0.2679706941643758 2210 5 0.0862760584140716 2210 6 0.039676674543606794 2210 7 0.05306170750461803 2210 8 -0.07814504893514851 2210 9 0.15447700613246695 2210 10 -0.006222141847302964 2210 11 -0.03774274450249493 2210 12 0.031258238947536654 2210 13 0.07151312487135897 2210 14 0.019876640092675757 2210 15 0.08859945797201618 2210 16 0.06231889570318572 2210 17 -0.05402281516386864 2210 18 -0.061951317334767 2210 19 0.03295726301421098 2210 20 0.06336920354665665 2210 21 0.005678151266792497 2210 22 0.005365211196796338 2210 23 0.019512638924148007 2210 24 0.03573376928028438 2210 25 0.03861707450357699 2211 1 -0.14816887134799828 2211 2 -0.0925664565397355 2211 3 0.1799987707660584 2211 4 0.1179324138997373 2211 5 0.017928305443476233 2211 6 -0.060711438659765804 2211 7 -0.10219330986532782 2211 8 -0.042268696402679495 2211 9 -0.17230368999304346 2211 10 0.0194579413514262 2211 11 -0.08879224331868585 2211 12 -0.05962574751053751 2211 13 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-0.12071396314269833 2214 10 -0.18310722078126673 2214 11 -0.07075242027910633 2214 12 -0.04747881757936767 2214 13 -0.197985262499202 2214 14 0.01907881289865543 2214 15 0.008927925125298941 2214 16 0.001964965979880162 2214 17 -0.15460421051270856 2214 18 -0.07301310650468906 2214 19 -0.0033888581876269086 2214 20 -0.007808864693032547 2214 21 0.0909073143969934 2214 22 0.27128356122225483 2214 23 -0.05522509660462305 2214 24 -0.22324263540482292 2214 25 -0.10971512715997989 2215 1 0.02714395881925924 2215 2 -0.13859479066243452 2215 3 -0.2357990997266164 2215 4 -0.03267003146671499 2215 5 -0.08321015997935177 2215 6 0.010305182071199681 2215 7 -0.4185802813541244 2215 8 -0.3616820863556782 2215 9 -0.48052475410278817 2215 10 -0.23149332161909086 2215 11 -0.7382716434235732 2215 12 -0.6597140321701076 2215 13 -0.3847804409510829 2215 14 -0.5214019119585132 2215 15 -0.6381681288490029 2215 16 -0.6219918168814615 2215 17 -0.540067255926779 2215 18 -0.6695050045099009 2215 19 -0.6533886956927404 2215 20 -0.8112241821105357 2215 21 -0.739318845978957 2215 22 -0.6449153910107484 2215 23 -0.7387370659775883 2215 24 -0.8227333764464859 2215 25 -0.7680428273374247 2216 1 -0.7192524914127851 2216 2 -0.6431313160038492 2216 3 -2.248030885030296 2216 4 -2.145456131351075 2216 5 -1.0733871938829367 2216 6 -0.8867030678400027 2216 7 -1.0035286120979663 2216 8 -1.009501271733761 2216 9 -0.967750100738769 2216 10 -1.0175317650943256 2216 11 -0.9892669015341937 2216 12 -1.1772650828091784 2216 13 -0.9622750669781155 2216 14 -1.4787341657278716 2216 15 -1.2945385382472665 2216 16 -1.1714323681997456 2216 17 -1.2729879534238648 2216 18 -1.177798681822395 2216 19 -1.312564344230701 2216 20 -1.2130197708235926 2216 21 -1.204511324268445 2216 22 -1.135673167490502 2216 23 -1.2617226888113748 2216 24 -1.4268529454513228 2216 25 -1.277310300534893 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12794.0 3 7.0 4 184.0 5 1224.0 6 1716.0 7 1176.0 8 936.0 9 889.0 10 736.0 11 703.0 12 642.0 13 645.0 14 618.0 15 661.0 16 719.0 17 721.0 18 808.0 19 1036.0 20 1186.0 21 1657.0 22 2050.0 23 2627.0 24 3075.0 25 3659.0 26 4550.0 27 5025.0 28 5821.0 29 7327.0 30 9632.0 31 12410.0 32 16555.0 33 22286.0 34 30885.0 35 44032.0 36 66492.0 37 116981.0 38 276788.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.67851198503238 19.47841114796321 11.19443330782034 27.648643559184073 2 14.219268901195518 20.67245419053881 40.5001647576544 24.608112150611277 3 18.823948843595208 24.454035848927795 29.279456273562367 27.44255903391463 4 12.230509379555345 17.38755834254831 37.841162649240886 32.54076962865546 5 12.990391645859184 38.56053687854397 35.37858492941637 13.070486546180474 6 31.932402152999344 37.87963662047298 17.61550451788889 12.572456708638788 7 28.31359757253935 31.70187748909539 21.3970036032619 18.587521335103357 8 24.943226035470644 38.46794489625952 19.675573011242534 16.913256057027304 9 26.0616357830384 15.829913758447804 19.835405304955287 38.273045153558506 10 14.82515806463357 29.83416865890805 33.922005266798536 21.418668009659843 11 34.40211521425191 22.685157093795127 24.268988993119184 18.643738698833783 12 23.176558745722396 25.044747955245978 31.6606193723758 20.11807392665583 13 29.02759975426435 22.13319782478441 25.64479063170748 23.19441178924376 14 20.584358357110244 22.730271989661023 27.34731582563902 29.33805382758971 15 22.734719386439416 31.58787319828771 23.011100762399998 22.66630665287288 16 21.861509290860827 28.591277967387178 27.975578308684113 21.571634433067878 17 21.387030716723547 29.23488813045127 27.766856276071294 21.611224876753887 18 21.294023778680494 28.13274559117537 29.631275730152325 20.941954899991806 19 23.363483711059978 27.114862169340658 27.287785684056605 22.23386843554276 20 23.25911229023416 27.842068299135224 28.069145555459485 20.829673855171126 21 22.58336114566583 27.054977396158865 27.071057981128067 23.290603477047238 22 22.75014296484051 28.42444402983377 27.136471127169692 21.688941878156033 23 22.19208710464723 28.093918419787244 27.522514118252023 22.191480357313505 24 22.92658952873863 27.152439431355536 28.350008797849686 21.57096224205615 25 22.513694193601236 27.721052575691612 27.987275890786243 21.777977339920906 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 144.0 1 144.0 2 155.0 3 166.0 4 166.0 5 166.0 6 311.0 7 456.0 8 456.0 9 456.0 10 768.5 11 1081.0 12 1081.0 13 1081.0 14 1812.0 15 2543.0 16 2543.0 17 2543.0 18 4809.0 19 7075.0 20 7075.0 21 7075.0 22 11276.5 23 15478.0 24 15478.0 25 15478.0 26 22617.5 27 29757.0 28 29757.0 29 29757.0 30 40024.0 31 50291.0 32 50291.0 33 50291.0 34 57155.0 35 64019.0 36 64019.0 37 64019.0 38 69546.0 39 75073.0 40 75073.0 41 75073.0 42 79287.0 43 83501.0 44 83501.0 45 83501.0 46 86174.0 47 88847.0 48 88847.0 49 88847.0 50 85319.0 51 81791.0 52 81791.0 53 81791.0 54 72024.0 55 62257.0 56 62257.0 57 62257.0 58 53241.0 59 44225.0 60 44225.0 61 44225.0 62 35768.0 63 27311.0 64 27311.0 65 27311.0 66 21075.5 67 14840.0 68 14840.0 69 14840.0 70 10724.5 71 6609.0 72 6609.0 73 6609.0 74 4566.5 75 2524.0 76 2524.0 77 2524.0 78 1715.0 79 906.0 80 906.0 81 906.0 82 581.0 83 256.0 84 256.0 85 256.0 86 172.0 87 88.0 88 88.0 89 88.0 90 50.5 91 13.0 92 13.0 93 13.0 94 7.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11604042757484609 2 0.10769765173613165 3 0.014865309676254792 4 0.0 5 0.005309039170090997 6 0.35024489839257467 7 0.01941591467918993 8 0.00910121000587028 9 0.008797836339007938 10 0.004398918169503969 11 0.004398918169503969 12 7.584341671558568E-4 13 0.0012134946674493706 14 0.0 15 0.0028820498351922554 16 4.55060500293514E-4 17 4.55060500293514E-4 18 0.0010618078340181992 19 3.0337366686234265E-4 20 9.10121000587028E-4 21 0.011073138840475508 22 0.0 23 0.0 24 1.5168683343117132E-4 25 0.004853978669797482 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 659253.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.08629259394995 #Duplication Level Percentage of deduplicated Percentage of total 1 91.73599867798579 76.22004027557333 2 6.250647003939126 10.386861717415657 3 1.053157356009328 2.625088206865852 4 0.32495405657174364 1.0799691129564342 5 0.174566960827602 0.725206079227937 6 0.10171771779490504 0.5070808837617785 7 0.07227892728560237 0.4203771670579874 8 0.045500765843592084 0.30243919553035964 9 0.031972740411614416 0.23908468183828227 >10 0.17686364491932477 2.682754043381486 >50 0.017541650069179553 1.0106610931025561 >100 0.01260783192179651 2.2005889103998055 >500 0.0016444998153146851 1.0325081184546303 >1k 5.481666051048949E-4 0.5673405144339 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1276 0.19355239945817462 No Hit CTGTAGGACGTGGAATATGGCAAGA 1240 0.18809167345465247 No Hit GTATCAACGCAGAGTACTTTTTTTT 1221 0.1852096236194602 No Hit CTTTAGGACGTGAAATATGGCGAGG 986 0.14956321776313494 No Hit GTCCTAAAGTGTGTATTTCTCATTT 968 0.14683285476137384 No Hit GTCCTACAGTGTGCATTTCTCATTT 941 0.14273731025873224 No Hit GGTATCAACGCAGAGTACTTTTTTT 820 0.1243832034135605 No Hit CTGTAGGACCTGGAATATGGCGAGA 745 0.11300669090622265 No Hit TATCAACGCAGAGTACTTTTTTTTT 717 0.10875945957014985 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.033736668623427E-4 0.0 10 0.0 0.0 0.0 3.033736668623427E-4 0.0 11 0.0 0.0 0.0 3.033736668623427E-4 0.0 12 0.0 0.0 0.0 3.033736668623427E-4 0.0 13 0.0 0.0 0.0 3.033736668623427E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTCGAG 35 1.00203215E-4 18.977243 18 ATGTCGA 40 2.7834196E-4 16.605087 17 GTCGAGG 35 0.0021858441 16.266209 19 TATGTCG 35 0.0021858441 16.266209 16 GTTCCAA 45 6.5810827E-4 14.828692 1 GTCCTAT 95 4.651156E-9 14.048236 1 TTAGAAC 70 7.0662336E-6 13.60471 3 TCTGAAC 50 0.0014705457 13.332615 3 TCCTATA 115 3.5470293E-10 13.262931 2 GGATAGA 55 0.0029872418 12.132567 1 GTCCTAC 695 0.0 11.795898 1 GACGTGA 330 0.0 11.788895 7 TAGGCCT 90 7.241948E-6 11.639584 4 TAGGACC 360 0.0 11.639584 4 AGGACGT 680 0.0 11.624023 5 GGACGTG 665 0.0 11.599805 6 TTTAGGA 420 0.0 11.575459 2 TAGGACG 695 0.0 11.510172 4 GTTTAGA 75 2.0052382E-4 11.439278 1 TTAGGAC 420 0.0 11.337258 3 >>END_MODULE