FastQCFastQC Report
Thu 26 May 2016
SRR1926840_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926840_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485181
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT6900.14221496719780866No Hit
GTCCTACAGTGGACATTTCTAAATT6150.1267568185893512No Hit
CTGTAGGACGTGGAATATGGCAAGA5050.10408486729694691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT901.2551027E-1015.9025691
TATACTG1152.3646862E-1114.0684075
TATTAAG500.001455319813.349852
TTAGAGT500.001471318513.3305034
ATACTCC500.001477068913.3236076
CTCTTAA801.8960473E-613.1196191
TAGGACA1550.012.9004874
TAGGACC1600.012.4973474
GTAGGAC3600.012.4479743
TGTAGGA3700.012.3705172
GTTACAC701.0515725E-412.2587963
TAATACT701.064882E-412.2423014
ATAGGAC550.002980846212.1349693
GCTAGAC550.002980846212.1349693
GTCTTGT751.9860148E-411.449851
GGACGTG2500.011.4202366
TGGCGAG3000.011.37903418
GGCGAGA1751.8189894E-1211.37903319
GTCTTAG855.0743474E-511.2253431
TAGAACT855.1826755E-511.2021044
Produced by FastQC (version 0.11.5)