##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926840_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 485181 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.32323607066229 31.0 28.0 31.0 22.0 34.0 2 28.44590369367308 31.0 28.0 31.0 22.0 34.0 3 28.046799029640486 31.0 28.0 31.0 16.0 34.0 4 31.722546843343 35.0 32.0 37.0 19.0 37.0 5 31.86434340998514 35.0 32.0 37.0 19.0 37.0 6 31.071167255106857 35.0 32.0 37.0 17.0 37.0 7 31.14122976785983 35.0 32.0 37.0 17.0 37.0 8 31.03193035176563 35.0 32.0 37.0 13.0 37.0 9 33.17829634713643 37.0 33.0 39.0 17.0 39.0 10 33.41856132041444 37.0 33.0 39.0 18.0 39.0 11 33.59324458294945 38.0 34.0 39.0 22.0 39.0 12 33.469031145077814 38.0 34.0 39.0 18.0 39.0 13 33.60004616833718 38.0 34.0 39.0 22.0 39.0 14 34.63515265437022 39.0 34.0 41.0 21.0 41.0 15 34.17599823570997 39.0 33.0 41.0 17.0 41.0 16 34.28529765180417 39.0 34.0 41.0 17.0 41.0 17 34.5217496150921 39.0 34.0 41.0 19.0 41.0 18 34.57139500516302 39.0 34.0 41.0 19.0 41.0 19 33.92400568035434 39.0 33.0 41.0 11.0 41.0 20 34.193599914258805 39.0 34.0 41.0 18.0 41.0 21 34.19282700682838 39.0 34.0 41.0 16.0 41.0 22 34.219091431857386 39.0 34.0 41.0 16.0 41.0 23 34.20593139467539 39.0 34.0 41.0 10.0 41.0 24 32.898056601556945 39.0 32.0 40.0 2.0 41.0 25 33.47359645163351 39.0 34.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.18917565797438485 1101 2 -0.1853368539918847 1101 3 -0.8614533190861486 1101 4 -0.5934220912851593 1101 5 -0.22359676148996144 1101 6 -2.6845528832465604 1101 7 -0.6073738529150141 1101 8 -0.165950516823127 1101 9 -0.152721544359828 1101 10 -9.103980814026489 1101 11 -3.7593110309279076 1101 12 -1.6539619400036791 1101 13 -0.6372419170577928 1101 14 -0.40796585893027526 1101 15 -1.8634521226112852 1101 16 -0.33188726415946235 1101 17 -0.28040045780659995 1101 18 -0.07253569681550687 1101 19 -0.5181167188540385 1101 20 0.04207662033070392 1101 21 -0.042742304019753874 1101 22 0.042220203665387146 1101 23 0.0016464722242872654 1101 24 -0.13182379699760816 1101 25 -0.1355658426956765 1102 1 -0.49553403947727404 1102 2 -0.5928513048589359 1102 3 -0.8050949375832595 1102 4 -0.7364856751001874 1102 5 -0.4793771083107714 1102 6 -1.6065182011656347 1102 7 -0.7793391708340884 1102 8 -0.13993895612948748 1102 9 -0.25243252701879015 1102 10 -0.33663977356405894 1102 11 -0.6263630540492926 1102 12 -0.3765052926048327 1102 13 -0.5433112812196441 1102 14 -0.6391797317626384 1102 15 -0.20449258503903422 1102 16 -0.26685836242536 1102 17 -0.4639264693672942 1102 18 -0.8817842517287957 1102 19 -0.4241860830158899 1102 20 -0.4015649981664069 1102 21 -0.46904288205443834 1102 22 -0.4751208367970392 1102 23 -0.9997986144809161 1102 24 -4.110147496419575 1102 25 -2.2714039929846948 1103 1 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-0.11054022086744908 2214 23 -0.39717343084163304 2214 24 -1.5553509069615004 2214 25 -0.6550419043710178 2215 1 0.7660675046719128 2215 2 0.2330029813805723 2215 3 0.6516290559623634 2215 4 1.014026658348886 2215 5 1.0728928182264 2215 6 2.1212131658966022 2215 7 1.4156045575917346 2215 8 -0.142107437239531 2215 9 0.4266952465604632 2215 10 0.08818167567577717 2215 11 0.03068331808520952 2215 12 0.032915515282034846 2215 13 0.40340944428537284 2215 14 0.5159517728296379 2215 15 0.37053958473567405 2215 16 0.7401406912045516 2215 17 0.3525373064451216 2215 18 -0.6385347981043381 2215 19 0.6637037840157092 2215 20 -3.3556098792573863 2215 21 -1.5680832355474976 2215 22 -0.903802659002011 2215 23 -0.6240590500834955 2215 24 -0.1364075643675875 2215 25 -0.20715295299885383 2216 1 -0.4453515022092489 2216 2 -1.0082385204895346 2216 3 -0.0634950322322112 2216 4 -0.4041867196190019 2216 5 -0.383962512089969 2216 6 -1.4120416436832954 2216 7 -10.141504941716015 2216 8 -11.283024512023072 2216 9 -6.025729690930493 2216 10 -3.4420685671279294 2216 11 -2.389497286547691 2216 12 -1.9993866771838142 2216 13 -1.6814210063996455 2216 14 -1.725541831617285 2216 15 -2.8082920644697857 2216 16 -1.8057895053114734 2216 17 -2.003964666980785 2216 18 -1.7728889402323347 2216 19 -1.2604298716782907 2216 20 -1.9633589338727262 2216 21 -4.960516025413799 2216 22 -3.1862691314257106 2216 23 -2.9659514725412883 2216 24 -2.88536299180544 2216 25 -2.512961644338514 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26018.0 3 12.0 4 228.0 5 1201.0 6 1632.0 7 1330.0 8 1040.0 9 886.0 10 917.0 11 844.0 12 842.0 13 796.0 14 818.0 15 872.0 16 933.0 17 965.0 18 1173.0 19 1440.0 20 1879.0 21 2443.0 22 3182.0 23 3800.0 24 4501.0 25 5126.0 26 5806.0 27 6582.0 28 7366.0 29 8639.0 30 10484.0 31 12777.0 32 16392.0 33 21418.0 34 28866.0 35 39758.0 36 57670.0 37 89902.0 38 116643.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.09057483040974 17.91645665843159 12.68270716451701 25.31026134664166 2 14.762766679806738 19.140061462510552 41.91524447755834 24.181927380124367 3 19.274445214979195 23.458861700019813 29.591011161746252 27.675681923254743 4 12.208251862485458 16.574470542616474 37.333036325685384 33.88424126921269 5 13.206092391931787 37.16870351128923 36.200996157208124 13.424207939570861 6 32.475730457065225 36.13538580790932 17.887616915392115 13.501266819633335 7 28.251353804609657 31.67665004952061 21.450798842933853 18.62119730293588 8 25.091278980817975 37.62926881711538 20.022866263371387 17.25658593869526 9 26.069272886191598 16.02940448900836 19.7734056764022 38.127916948397846 10 15.102563415302969 29.14454544892241 33.683149250438646 22.06974188533598 11 34.91422493228284 22.82800047824599 23.208742006901584 19.049032582569584 12 23.11461554318697 24.732014017728304 31.02618016903731 21.127190270047414 13 28.912617756797427 21.79320154191833 25.377543237616212 23.91663746366803 14 21.07421150278293 21.936507936507937 26.814677386105956 30.174603174603178 15 23.579406077746672 30.128176057586465 22.83710632964623 23.455311535020634 16 22.72561416818014 28.175152086120637 26.805500868916297 22.293732876782926 17 22.206784238138923 28.412166688999065 26.977396180129666 22.40365289273235 18 22.116336021372383 27.85645376737743 28.32768515311928 21.699525058130906 19 24.154402139068303 26.906988873793736 26.569512504355032 22.369096482782933 20 23.52123883224559 27.39052817757915 27.343115468499406 21.74511752167585 21 23.174552785530825 26.751237853370547 26.788754808148184 23.28545455295044 22 23.292895352552538 27.325218251718702 26.944063656321827 22.437822739406933 23 22.96944347671928 27.19949041539803 26.98943106693245 22.841635040950234 24 23.647941094015405 26.94285031085605 27.154966275829086 22.25424231929946 25 23.268963583817413 26.789785493719005 27.254830823270403 22.68642009919318 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 147.0 1 147.0 2 199.0 3 251.0 4 251.0 5 251.0 6 343.0 7 435.0 8 435.0 9 435.0 10 614.0 11 793.0 12 793.0 13 793.0 14 1373.5 15 1954.0 16 1954.0 17 1954.0 18 3416.5 19 4879.0 20 4879.0 21 4879.0 22 7802.0 23 10725.0 24 10725.0 25 10725.0 26 15290.5 27 19856.0 28 19856.0 29 19856.0 30 26238.5 31 32621.0 32 32621.0 33 32621.0 34 37635.5 35 42650.0 36 42650.0 37 42650.0 38 47300.0 39 51950.0 40 51950.0 41 51950.0 42 55696.5 43 59443.0 44 59443.0 45 59443.0 46 61892.0 47 64341.0 48 64341.0 49 64341.0 50 63046.5 51 61752.0 52 61752.0 53 61752.0 54 55902.0 55 50052.0 56 50052.0 57 50052.0 58 43278.0 59 36504.0 60 36504.0 61 36504.0 62 30103.0 63 23702.0 64 23702.0 65 23702.0 66 18454.5 67 13207.0 68 13207.0 69 13207.0 70 9696.5 71 6186.0 72 6186.0 73 6186.0 74 4329.5 75 2473.0 76 2473.0 77 2473.0 78 1687.5 79 902.0 80 902.0 81 902.0 82 580.5 83 259.0 84 259.0 85 259.0 86 167.0 87 75.0 88 75.0 89 75.0 90 45.5 91 16.0 92 16.0 93 16.0 94 10.5 95 5.0 96 5.0 97 5.0 98 4.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2211545794249981 2 0.13561949045820013 3 0.13788668558744058 4 0.07110748359890433 5 0.02411471182919364 6 0.022259733996178744 7 0.3182317526861109 8 0.30895686352103646 9 0.017519235089585125 10 0.033801818290493654 11 0.015458148608457463 12 0.01669480049713406 13 0.014633714016006397 14 0.01669480049713406 15 0.013809279423555334 16 0.01937421292260002 17 0.01772534373769789 18 0.014221496719780865 19 0.022671951292404277 20 0.016282583200908526 21 0.013809279423555334 22 0.015664257256570228 23 0.016488691849021294 24 0.014221496719780865 25 0.013809279423555334 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 485181.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.35573514015802 #Duplication Level Percentage of deduplicated Percentage of total 1 93.44095952011526 81.62603711081417 2 4.834807027018172 8.446962442119487 3 0.8934714083647936 2.341495551132566 4 0.33094778315321205 1.1564074756141776 5 0.15886815641008672 0.6939022296782367 6 0.07797781520563091 0.40870856231467684 7 0.05384270240119391 0.3292428195132332 8 0.03475472404415119 0.2428819574776122 9 0.028301122484930608 0.22250388239664917 >10 0.12477636615969682 1.9045727243578467 >50 0.011593061929932116 0.7084908859380674 >100 0.008990533733963824 1.5447079961763155 >500 7.097789789967384E-4 0.3740863624669882 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 690 0.14221496719780866 No Hit GTCCTACAGTGGACATTTCTAAATT 615 0.1267568185893512 No Hit CTGTAGGACGTGGAATATGGCAAGA 505 0.10408486729694691 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 2.0610864811276616E-4 13 0.0 0.0 0.0 0.0 2.0610864811276616E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAT 90 1.2551027E-10 15.902569 1 TATACTG 115 2.3646862E-11 14.068407 5 TATTAAG 50 0.0014553198 13.34985 2 TTAGAGT 50 0.0014713185 13.330503 4 ATACTCC 50 0.0014770689 13.323607 6 CTCTTAA 80 1.8960473E-6 13.119619 1 TAGGACA 155 0.0 12.900487 4 TAGGACC 160 0.0 12.497347 4 GTAGGAC 360 0.0 12.447974 3 TGTAGGA 370 0.0 12.370517 2 GTTACAC 70 1.0515725E-4 12.258796 3 TAATACT 70 1.064882E-4 12.242301 4 ATAGGAC 55 0.0029808462 12.134969 3 GCTAGAC 55 0.0029808462 12.134969 3 GTCTTGT 75 1.9860148E-4 11.44985 1 GGACGTG 250 0.0 11.420236 6 TGGCGAG 300 0.0 11.379034 18 GGCGAGA 175 1.8189894E-12 11.379033 19 GTCTTAG 85 5.0743474E-5 11.225343 1 TAGAACT 85 5.1826755E-5 11.202104 4 >>END_MODULE