FastQCFastQC Report
Thu 26 May 2016
SRR1926840_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926840_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485181
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12500.2576358101409577No Hit
GGTATCAACGCAGAGTACTTTTTTT7860.1620013974166342No Hit
TATCAACGCAGAGTACTTTTTTTTT6280.12943623101481716No Hit
GTCCTACAGTGGACATTTCTAAATT5990.12345908021954694No Hit
CTGTAGGACGTGGAATATGGCAAGA5570.11480251699881076No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGG350.002127105116.3314063
GTGTTAA400.00514666814.3018231
CTTAGTT400.005175043414.2899813
CCAACGT400.005315267514.23252619
ACAATAC551.9098203E-413.8569513
TCCAACG707.330247E-613.55478718
TAGGACC1500.013.3345564
TCAGGAC655.2962554E-513.1907523
TATACTG1001.3867611E-712.3820865
AGGACCT2900.012.1521815
TTACCCA550.003004231512.1223224
GGCGAGA2350.012.11278819
CCTGAAC951.0004096E-612.03366853
TGGCGAG3200.011.86043818
CAGAAGT1052.6151793E-711.7924624
TCTTAGA752.0015238E-411.4402732
AATCCCG855.3718584E-511.162765519
CTCTTAG600.00570933211.123641
GCTTAGA600.00570933211.123641
CCCATAC600.00570933211.123641
Produced by FastQC (version 0.11.5)