FastQCFastQC Report
Thu 26 May 2016
SRR1926839_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926839_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578609
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT13660.23608343458190245No Hit
CTGTAGGACGTGGAATATGGCAAGA11440.19771555575526822No Hit
GTCCTAAAGTGTGTATTTCTCATTT10480.181124040586994No Hit
GTCCTACAGTGTGCATTTCTCATTT9670.1671249496637626No Hit
CTTTAGGACGTGAAATATGGCGAGG8130.14050939408132262No Hit
GTATCAACGCAGAGTACTTTTTTTT6770.11700474759293408No Hit
CTGTAGGACCTGGAATATGGCGAGA5880.10162303040567983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG759.866872E-713.90493918
GTATAGA1009.424184E-913.3583341
TGTAGGA6500.012.7611642
GTTTAAG751.4098567E-512.7222221
CTGTAGG6500.012.4775651
AATCCCG1155.456968E-912.36605319
TAGGACC2850.012.364844
GTCTTAT550.0029655812.1439391
AGGACCT4250.012.0961725
CCAACGT550.003111619312.06626919
GTAGGAC6450.011.9731853
ACTGTTC2350.011.7380658
GGACCTG4150.011.4700426
GTATAAA1001.8238334E-611.4500011
CTTTAGG3250.011.4500011
TAGGACA2000.011.4291224
GACAGCC752.0291985E-411.4241637
AGGACGT5000.011.4241635
GTTCTAG1104.6746572E-711.2765151
CGTGGAA2800.011.1784419
Produced by FastQC (version 0.11.5)