##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926839_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 578609 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.50989701162616 31.0 28.0 31.0 23.0 34.0 2 28.673319979468 31.0 30.0 33.0 22.0 34.0 3 28.26062850733397 31.0 28.0 31.0 16.0 34.0 4 31.908926407988815 35.0 32.0 37.0 19.0 37.0 5 32.08176160412299 35.0 33.0 37.0 22.0 37.0 6 31.28073016493003 35.0 32.0 37.0 17.0 37.0 7 31.338285439735643 35.0 32.0 37.0 17.0 37.0 8 31.242668192164313 35.0 32.0 37.0 17.0 37.0 9 33.40768636505827 37.0 33.0 39.0 18.0 39.0 10 33.656498602683335 37.0 34.0 39.0 22.0 39.0 11 33.817403462441824 38.0 34.0 39.0 23.0 39.0 12 33.71341786940749 38.0 34.0 39.0 22.0 39.0 13 33.8356644988239 38.0 34.0 39.0 23.0 39.0 14 34.89644993423884 39.0 35.0 41.0 24.0 41.0 15 34.47369985603404 39.0 34.0 41.0 18.0 41.0 16 34.56397152481209 39.0 34.0 41.0 19.0 41.0 17 34.80549905030858 39.0 35.0 41.0 23.0 41.0 18 34.852646605911765 39.0 35.0 41.0 24.0 41.0 19 34.22634974568318 39.0 34.0 41.0 18.0 41.0 20 34.506725612633055 39.0 34.0 41.0 18.0 41.0 21 34.49518932474262 39.0 34.0 41.0 18.0 41.0 22 34.521218992445675 39.0 34.0 41.0 18.0 41.0 23 34.52673567123913 39.0 35.0 41.0 18.0 41.0 24 33.22426370830734 39.0 33.0 41.0 2.0 41.0 25 33.806181722026444 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.08049514814073433 1101 2 -0.17764707336428742 1101 3 -0.8962702171938766 1101 4 -0.3802681692376737 1101 5 -0.16525952834103563 1101 6 -2.32491317681529 1101 7 -0.7413587294235597 1101 8 -0.34193244721913274 1101 9 -0.30880528264749785 1101 10 -9.009973669321187 1101 11 -3.9618591000233785 1101 12 -1.3913699477401593 1101 13 -0.6600501762042228 1101 14 -0.636240228028143 1101 15 -2.3945257634775743 1101 16 -0.4813356415449306 1101 17 -0.2736448598745227 1101 18 -0.18292960364217947 1101 19 -0.876578333421584 1101 20 -0.2602537823831099 1101 21 -0.22626232723510498 1101 22 0.1143056961891844 1101 23 -0.024859560240052758 1101 24 -0.11991042229337268 1101 25 -0.2886686852375746 1102 1 -0.2124368928101319 1102 2 -0.5453571467174712 1102 3 -0.7938292408033192 1102 4 -0.56522044196171 1102 5 -0.6037010971393748 1102 6 -1.3199076158720509 1102 7 -0.5185649855915067 1102 8 0.30837607476617634 1102 9 0.036743613067372394 1102 10 -0.15002826767298671 1102 11 -0.4126899661776662 1102 12 -0.008272936693749955 1102 13 -0.3307098991681414 1102 14 -0.4546344896473826 1102 15 0.20676813486468149 1102 16 -0.11230404314044762 1102 17 -0.08850745971872698 1102 18 -0.30480611225274146 1102 19 0.25591736948591404 1102 20 -0.08312243162216504 1102 21 -0.31789613378263937 1102 22 -0.3091191897794374 1102 23 -0.8162252168229713 1102 24 -4.20480274221066 1102 25 -2.2045994703630143 1103 1 0.03183048121716325 1103 2 -0.2096667669897485 1103 3 0.02563689336921371 1103 4 -0.027874554161460452 1103 5 -0.4845071803618133 1103 6 0.39807427478803703 1103 7 0.3928940493171531 1103 8 0.6085760561150657 1103 9 0.3209051198402406 1103 10 0.30971863972128943 1103 11 0.28729656357603517 1103 12 -0.015311510446302634 1103 13 0.08999845815752394 1103 14 0.06941730122926515 1103 15 0.584276968237738 1103 16 0.363658968451368 1103 17 0.15015569974974596 1103 18 -0.0661281503158051 1103 19 -1.5574901143460878 1103 20 -0.11702156464158264 1103 21 -0.011045403354827954 1103 22 0.18103642857147406 1103 23 0.13461726566941223 1103 24 1.454579136311402 1103 25 0.7804988846386678 1104 1 -0.021437958024950632 1104 2 0.09086494775412035 1104 3 -1.0676317195412928 1104 4 -0.500504414705734 1104 5 -0.0759406972047536 1104 6 -1.2301393141851804 1104 7 -0.1322372150466684 1104 8 0.4833770610761903 1104 9 0.47786372308321035 1104 10 0.7532879884069104 1104 11 0.48225815391543847 1104 12 0.5736443372580879 1104 13 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24 -2.1643164281631293 2214 25 -1.1322717344877873 2215 1 0.3752473612193512 2215 2 0.10670039761173555 2215 3 0.5293164108999839 2215 4 0.7472783280380959 2215 5 0.7698450426280061 2215 6 1.3453945705762997 2215 7 0.6536350419604986 2215 8 -0.6764703228092479 2215 9 0.0052359280261455865 2215 10 -0.07254809527615436 2215 11 -0.1962689715503103 2215 12 -0.3552313922071022 2215 13 -4.597663281202813E-4 2215 14 -0.03564722260941977 2215 15 -0.13129495922541423 2215 16 0.12056943411931087 2215 17 0.03951096076421834 2215 18 -0.810365713142275 2215 19 0.49337628747934303 2215 20 -3.652228762679176 2215 21 -1.7622115648731054 2215 22 -0.9734176960672016 2215 23 -0.7345728837585952 2215 24 -0.6951497006661711 2215 25 -0.3384038117717054 2216 1 -0.39141940026739164 2216 2 -0.9502494871344673 2216 3 -0.22567041385070752 2216 4 -0.09596607354767528 2216 5 -0.35477792905208005 2216 6 -1.2753080651373025 2216 7 -9.509517118657122 2216 8 -11.350292112272651 2216 9 -6.056745109855427 2216 10 -3.3543400808938486 2216 11 -2.310106159793758 2216 12 -1.820361784032091 2216 13 -1.6318533654041971 2216 14 -1.7922184907994634 2216 15 -3.0526123134798446 2216 16 -2.0103600206937102 2216 17 -2.1102412269719437 2216 18 -1.8955131915446373 2216 19 -1.2754172882709938 2216 20 -2.1524634423627447 2216 21 -4.676057097283081 2216 22 -3.378708087285343 2216 23 -3.2169813894985317 2216 24 -3.1483801731830496 2216 25 -2.693305867376097 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29051.0 3 14.0 4 221.0 5 1340.0 6 1822.0 7 1553.0 8 1155.0 9 1015.0 10 903.0 11 871.0 12 952.0 13 946.0 14 950.0 15 954.0 16 971.0 17 1167.0 18 1338.0 19 1688.0 20 2209.0 21 2844.0 22 3544.0 23 4274.0 24 5091.0 25 5723.0 26 6643.0 27 7496.0 28 8315.0 29 9929.0 30 11908.0 31 14553.0 32 19057.0 33 25200.0 34 34101.0 35 47844.0 36 69429.0 37 109255.0 38 144283.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.11101257644856 18.577324980211277 12.865008859459357 25.446653583880806 2 13.829268039417256 19.45681858301403 43.37559577583253 23.338317601736186 3 19.064398941482096 24.011187475012505 29.903130727653178 27.021282855852224 4 11.665776043801351 16.992911333738004 38.59008347643827 32.751229146022375 5 12.543841908110473 37.75200994297311 36.9183006366476 12.78584751226882 6 31.400247188874776 37.14678351958929 18.52965834348882 12.92331094804712 7 27.70689153017212 31.741571085807802 22.3194596618726 18.232077722147483 8 24.09832996460232 38.98424959349875 20.558562181689588 16.358858260209335 9 25.946982137239377 15.94857239655536 20.29257314328582 37.811872322919434 10 14.541223415750773 29.515713255774152 34.86861100593537 21.07445232253971 11 34.18485785549954 22.764899374450113 24.550974796510474 18.49926797353988 12 23.13561314231982 24.829567453952098 32.185996183417224 19.84882322031086 13 29.01167775211914 22.291611930804653 25.604802466916492 23.091907850159714 14 20.27729186688884 22.703811769261083 27.62219026349814 29.39670610035193 15 22.77551344941905 31.819171774369188 23.129509211149486 22.275805565062278 16 21.427966431293914 28.46010907236632 28.749989196477188 21.36193529986258 17 21.252690077180368 29.143237426860154 28.145337631694943 21.45873486426454 18 21.003596991732625 28.13760178656927 30.279894700270855 20.57890652142725 19 23.676090545294254 27.02477981659306 27.018038185291143 22.281091452821546 20 23.215683969806076 28.015978510942446 28.207672243473514 20.560665275777968 21 22.551076848618937 27.2411587789954 26.973070142081788 23.23469423030387 22 22.44545198409063 28.783617704964502 27.495345938910372 21.275584372034494 23 21.923124125998026 28.3023662226002 27.853978361915033 21.92053128948674 24 23.18834567491513 27.401077209212282 28.23352900098871 21.177048114883878 25 22.388157735533362 27.77639498183352 28.11984692448897 21.71560035814415 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 153.0 1 153.0 2 223.5 3 294.0 4 294.0 5 294.0 6 369.5 7 445.0 8 445.0 9 445.0 10 730.5 11 1016.0 12 1016.0 13 1016.0 14 1710.5 15 2405.0 16 2405.0 17 2405.0 18 4355.0 19 6305.0 20 6305.0 21 6305.0 22 10400.5 23 14496.0 24 14496.0 25 14496.0 26 21187.0 27 27878.0 28 27878.0 29 27878.0 30 37150.0 31 46422.0 32 46422.0 33 46422.0 34 52493.5 35 58565.0 36 58565.0 37 58565.0 38 63229.0 39 67893.0 40 67893.0 41 67893.0 42 70999.5 43 74106.0 44 74106.0 45 74106.0 46 75869.0 47 77632.0 48 77632.0 49 77632.0 50 73605.0 51 69578.0 52 69578.0 53 69578.0 54 61033.5 55 52489.0 56 52489.0 57 52489.0 58 44470.5 59 36452.0 60 36452.0 61 36452.0 62 29375.0 63 22298.0 64 22298.0 65 22298.0 66 17060.0 67 11822.0 68 11822.0 69 11822.0 70 8558.5 71 5295.0 72 5295.0 73 5295.0 74 3702.5 75 2110.0 76 2110.0 77 2110.0 78 1393.5 79 677.0 80 677.0 81 677.0 82 434.5 83 192.0 84 192.0 85 192.0 86 127.5 87 63.0 88 63.0 89 63.0 90 39.0 91 15.0 92 15.0 93 15.0 94 9.5 95 4.0 96 4.0 97 4.0 98 4.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21776363658359962 2 0.13670717185525977 3 0.14171919206234262 4 0.06256383844703418 5 0.019011111130314253 6 0.01797414143229711 7 0.3084984851600995 8 0.2998570710099566 9 0.015036060621248548 10 0.03663959599660565 11 0.013480606074222834 12 0.013999090923231405 13 0.01261646465920855 14 0.014690404055242834 15 0.012270808093202837 16 0.016245858602268543 17 0.016245858602268543 18 0.01209797981019998 19 0.01970242426232568 20 0.014171919206234262 21 0.011925151527197123 22 0.014171919206234262 23 0.015900202036262828 24 0.013307777791219976 25 0.012270808093202837 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 578609.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.48382819585763 #Duplication Level Percentage of deduplicated Percentage of total 1 93.82293512424215 80.20343666991813 2 4.58675678015829 7.841870571424728 3 0.749397345347957 1.9218406176046963 4 0.27918237246437566 0.9546231185222649 5 0.1425953385579927 0.6094797711410801 6 0.09200416702695671 0.47189210444612145 7 0.05502658931507471 0.32927184550497035 8 0.046092129265836376 0.31521053274736144 9 0.034140820125778105 0.2626639201887924 >10 0.16170429717537346 2.613991194594433 >50 0.014576433982246525 0.8480048882548816 >100 0.013969007289937297 2.398286004331097 >500 0.0010122469050249502 0.6136760117745168 >1k 6.0734814301497E-4 0.6157527495470194 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1366 0.23608343458190245 No Hit CTGTAGGACGTGGAATATGGCAAGA 1144 0.19771555575526822 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1048 0.181124040586994 No Hit GTCCTACAGTGTGCATTTCTCATTT 967 0.1671249496637626 No Hit CTTTAGGACGTGAAATATGGCGAGG 813 0.14050939408132262 No Hit GTATCAACGCAGAGTACTTTTTTTT 677 0.11700474759293408 No Hit CTGTAGGACCTGGAATATGGCGAGA 588 0.10162303040567983 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 1.7282828300285686E-4 0.0 13 0.0 0.0 0.0 1.7282828300285686E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 75 9.866872E-7 13.904939 18 GTATAGA 100 9.424184E-9 13.358334 1 TGTAGGA 650 0.0 12.761164 2 GTTTAAG 75 1.4098567E-5 12.722222 1 CTGTAGG 650 0.0 12.477565 1 AATCCCG 115 5.456968E-9 12.366053 19 TAGGACC 285 0.0 12.36484 4 GTCTTAT 55 0.00296558 12.143939 1 AGGACCT 425 0.0 12.096172 5 CCAACGT 55 0.0031116193 12.066269 19 GTAGGAC 645 0.0 11.973185 3 ACTGTTC 235 0.0 11.738065 8 GGACCTG 415 0.0 11.470042 6 GTATAAA 100 1.8238334E-6 11.450001 1 CTTTAGG 325 0.0 11.450001 1 TAGGACA 200 0.0 11.429122 4 GACAGCC 75 2.0291985E-4 11.424163 7 AGGACGT 500 0.0 11.424163 5 GTTCTAG 110 4.6746572E-7 11.276515 1 CGTGGAA 280 0.0 11.178441 9 >>END_MODULE