FastQCFastQC Report
Thu 26 May 2016
SRR1926839_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926839_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578609
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT14300.2471444446940853No Hit
CTGTAGGACGTGGAATATGGCAAGA13260.2291703032617882No Hit
GTATCAACGCAGAGTACTTTTTTTT12680.2191462628476225No Hit
GTCCTACAGTGTGCATTTCTCATTT10180.17593919209690828No Hit
CTTTAGGACGTGAAATATGGCGAGG10110.17472939411588828No Hit
GTCCTAAAGTGTGTATTTCTCATTT9830.16989020219180828No Hit
GGTATCAACGCAGAGTACTTTTTTT8080.13964525266630834No Hit
CTGTAGGACCTGGAATATGGCGAGA7060.12201676780001694No Hit
TATCAACGCAGAGTACTTTTTTTTT6580.11372101021587981No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTCGA456.8185886E-414.75902316
CTCGAAC456.8185886E-414.75902318
CCTACAC954.6929927E-914.0380453
CTACACT1101.7826096E-1013.8521254
ATCCCGT551.9779989E-413.80064510
TAGGACC2500.013.7136044
CCAACGA707.339351E-613.55420419
GGGTAAT500.001459575413.3454071
CCCGTTT500.001512948813.28197212
GTACTGT801.9390263E-613.0945886
AATTTTA604.1261158E-412.65168415
ACTGTTC2850.012.6494978
GGACCTG4350.012.2599276
AGGACCT4900.012.2442895
AATACAA701.0639559E-412.2442895
TGCTCAT701.1016011E-412.19878410
ATAGAGG550.003002761912.1237653
TGAACAG802.788143E-511.9041715
TAGGACA2200.011.6877314
GGACATG1900.011.528256
Produced by FastQC (version 0.11.5)