FastQCFastQC Report
Thu 26 May 2016
SRR1926838_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926838_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644497
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT14910.23134320252848345No Hit
CTGTAGGACGTGGAATATGGCAAGA12440.19301874174744027No Hit
GTCCTAAAGTGTGTATTTCTCATTT11080.17191701435382942No Hit
GTCCTACAGTGTGCATTTCTCATTT10170.15779747617133982No Hit
GTATCAACGCAGAGTACTTTTTTTT9070.1407299025441546No Hit
CTTTAGGACGTGAAATATGGCGAGG8450.131109997408832No Hit
CTGTAGGACCTGGAATATGGCGAGA6690.10380187960533564No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTAC508.36564E-515.270241
TAGTACC653.2757325E-614.6531624
TAGGACC3350.014.2157554
TTAACCT751.4367357E-512.6994094
CTAGCCC550.002985368512.13351253
ACTTTTA550.003041670412.10329158
TGTAGGA8700.011.8346572
AACAGAT657.881632E-411.7152247
GATATAC2150.011.5414591
GTAGGAC8700.011.5059173
CTCTAGG1001.8197697E-611.452681
AGGACGT6250.011.4223445
GGACGTG6050.011.3279456
CTGTAGG7950.011.2846111
GACGTGG3950.011.0849757
GTATGAA700.001433126710.9073131
AATGTCC1408.172719E-910.8682618
TCCAACG700.001514497710.83537118
GTCCTAC8350.010.7440311
ATTTCTA5300.010.73398315
Produced by FastQC (version 0.11.5)