FastQCFastQC Report
Thu 26 May 2016
SRR1926838_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926838_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644497
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15330.23785991245886326No Hit
CTGTAGGACGTGGAATATGGCAAGA14930.2316535220489777No Hit
GTATCAACGCAGAGTACTTTTTTTT14900.23118804276823632No Hit
GTCCTAAAGTGTGTATTTCTCATTT10720.16633126298493242No Hit
CTTTAGGACGTGAAATATGGCGAGG10570.16400386658122534No Hit
GTCCTACAGTGTGCATTTCTCATTT10190.15810779569183409No Hit
GGTATCAACGCAGAGTACTTTTTTT9510.1475569319950287No Hit
CTGTAGGACCTGGAATATGGCGAGA8340.12940324004611348No Hit
TATCAACGCAGAGTACTTTTTTTTT7630.11838689706856664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG1101.891749E-1013.80390219
GTCTTAA500.001459989313.3452881
TCTTACA1009.573341E-913.3442482
TAGGACC3550.012.8707514
ATTATAC604.0126746E-412.6939673
CTAGACA604.0179503E-412.6919914
TAGAACC604.0179503E-412.6919914
TAGGACA2650.012.5722554
GGCGAGG3200.012.45586519
GTCTTAC1155.0367817E-912.4334981
TTAGGAC4900.012.2406113
CCTATAC701.067312E-412.240613
GTGCAGG1052.593133E-711.8019561
CTAGTAC657.868768E-411.7175073
GTCTTAT907.1650156E-611.6506491
TCCTAGA1251.7354978E-811.4379262
CTAGAGT1001.8779283E-611.42279154
GTAGGAC8400.011.33389953
TGGCGAG7400.011.28474818
ACAGCGT600.00592150911.0701638
Produced by FastQC (version 0.11.5)