FastQCFastQC Report
Thu 26 May 2016
SRR1926837_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926837_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503245
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT8470.16830768313644445No Hit
GTCCTACAGTGGACATTTCTAAATT7990.15876958539081362No Hit
CTGTAGGACGTGGAATATGGCAAGA6770.13452692028733518No Hit
GTCCTAAAGTGTGTATTTCTCATTT6210.12339913958409919No Hit
CTTTAGGACGTGAAATATGGCGAGG5670.11266877962026448No Hit
GGTATCAACGCAGAGTACTTTTTTT5190.10313068187463362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGTG1051.9363142E-812.6874797
GTCCTAC5000.012.5970411
GTATTGG701.0429088E-412.2698461
GTGTAGA959.79866E-712.05458451
TCCTACA5350.011.7635222
TATTGTA657.759511E-411.7361012
TATGAAC751.9978645E-411.44269853
TAGGACC1251.7673301E-811.4221494
GTGTAGG855.066166E-511.2273091
CCCTTAC600.005671329311.1337491
TACTATA600.00570521811.1248462
GGTATCA3350.011.1100091
TTAACCC600.005782101311.1048674
TGTAGAA2500.011.06127552
CCTACAG5200.011.0025953
AATGTCC1053.411571E-610.8673948
CATTTCT6100.010.7271814
TTAATCC803.694214E-410.7082654
TCCAACG803.822214E-410.66888118
CCAGCAC1105.7550133E-610.4024533
Produced by FastQC (version 0.11.5)