##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926837_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 503245 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.51263102465002 31.0 28.0 31.0 23.0 34.0 2 28.6748104799849 31.0 30.0 33.0 22.0 34.0 3 28.262075132390784 31.0 28.0 31.0 16.0 34.0 4 31.909394032727597 35.0 32.0 37.0 19.0 37.0 5 32.05750876809506 35.0 33.0 37.0 20.0 37.0 6 31.268288805651324 35.0 32.0 37.0 17.0 37.0 7 31.341092310902244 35.0 32.0 37.0 17.0 37.0 8 31.230152311498376 35.0 32.0 37.0 17.0 37.0 9 33.33777186062455 37.0 33.0 39.0 18.0 39.0 10 33.57494659658814 37.0 34.0 39.0 19.0 39.0 11 33.7341493705849 38.0 34.0 39.0 23.0 39.0 12 33.6249600095381 38.0 34.0 39.0 21.0 39.0 13 33.746932408667746 38.0 34.0 39.0 23.0 39.0 14 34.78981808065654 39.0 35.0 41.0 23.0 41.0 15 34.361259426323166 39.0 34.0 41.0 17.0 41.0 16 34.44392294011863 39.0 34.0 41.0 18.0 41.0 17 34.686150880783714 39.0 34.0 41.0 22.0 41.0 18 34.74841081381832 39.0 35.0 41.0 22.0 41.0 19 34.12360182416119 39.0 34.0 41.0 16.0 41.0 20 34.39006646861866 39.0 34.0 41.0 18.0 41.0 21 34.38388458901728 39.0 34.0 41.0 18.0 41.0 22 34.41796540452464 39.0 34.0 41.0 18.0 41.0 23 34.427879064869 39.0 34.0 41.0 17.0 41.0 24 33.129275005216144 39.0 32.0 41.0 2.0 41.0 25 33.6886824508937 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.20075001754529254 1101 2 -0.2975305750384365 1101 3 -1.3095135750305147 1101 4 -0.519275058939364 1101 5 -0.04412479269754499 1101 6 -2.072940282440438 1101 7 -0.41736528974285747 1101 8 0.10263417180891565 1101 9 -0.13086674493852968 1101 10 -9.126956137696531 1101 11 -3.777888678093092 1101 12 -1.3245008648562475 1101 13 -0.6099195840008989 1101 14 -0.4271186230685373 1101 15 -1.659271284812057 1101 16 0.03655892714347431 1101 17 0.15740731036220268 1101 18 0.19468863343848852 1101 19 -0.6422733715336122 1101 20 0.3293680736400404 1101 21 0.016989171177662854 1101 22 0.19236804439438515 1101 23 0.10660555699482899 1101 24 0.23894921872457076 1101 25 0.3020997212143257 1102 1 -0.43206657711915497 1102 2 -0.4795837954210107 1102 3 -0.7801270239123745 1102 4 -0.6949260909658932 1102 5 -0.5995857463625356 1102 6 -1.3329186170921368 1102 7 -0.3454604925827347 1102 8 0.058491024644308 1102 9 0.10248191519995942 1102 10 0.45171128751709944 1102 11 -0.10739095957895728 1102 12 0.10013801353334628 1102 13 -0.05680051420808496 1102 14 -0.26154821263488515 1102 15 0.43907419956044436 1102 16 0.27374383465659236 1102 17 0.13384819240684465 1102 18 -0.39116225752948 1102 19 0.19621457419390964 1102 20 0.07619328181831975 1102 21 0.025474116488240384 1102 22 0.046892634175044634 1102 23 -0.43597698369588755 1102 24 -3.861401089694912 1102 25 -2.2434462182863584 1103 1 -0.261052476473008 1103 2 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0.3863969360176718 2215 2 0.25934243998521467 2215 3 0.4705455375257088 2215 4 0.8583764287895868 2215 5 0.705403849193047 2215 6 1.5572804610935371 2215 7 1.1623217725393644 2215 8 -0.14318341024202041 2215 9 0.5382304193584986 2215 10 0.3549953320909083 2215 11 0.34041827282585757 2215 12 -0.09631475158550984 2215 13 0.34830187719349937 2215 14 0.3311777518020165 2215 15 0.4413884591594339 2215 16 0.45398705880268153 2215 17 -0.11212454211451472 2215 18 -0.8473306993261716 2215 19 0.4918142396958842 2215 20 -3.338606959331422 2215 21 -1.7392266179812594 2215 22 -0.7047263751662669 2215 23 -0.7224071248203501 2215 24 -0.2187953760018857 2215 25 0.16718900383718704 2216 1 -0.49681215259051825 2216 2 -0.9009710898609136 2216 3 -0.11784477790890335 2216 4 -0.015205759291788468 2216 5 -0.3049536468473839 2216 6 -1.4258910540310943 2216 7 -9.534687221613261 2216 8 -10.782973729329612 2216 9 -5.882977463767094 2216 10 -3.248793588428125 2216 11 -2.4178250624470294 2216 12 -1.9519242900317977 2216 13 -1.8433620279960756 2216 14 -2.0783053779647958 2216 15 -2.973914646654279 2216 16 -2.0195784299526665 2216 17 -2.1102850069820036 2216 18 -1.8785258231229776 2216 19 -1.2020753725405484 2216 20 -2.1298158078868 2216 21 -4.65568041172893 2216 22 -2.946370841296641 2216 23 -3.021075875423744 2216 24 -3.25586005196973 2216 25 -2.4900860314531883 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25841.0 3 7.0 4 211.0 5 1214.0 6 1673.0 7 1462.0 8 1047.0 9 972.0 10 877.0 11 810.0 12 822.0 13 812.0 14 820.0 15 875.0 16 921.0 17 1036.0 18 1162.0 19 1469.0 20 1856.0 21 2407.0 22 3143.0 23 3746.0 24 4449.0 25 5024.0 26 5738.0 27 6462.0 28 7250.0 29 8666.0 30 10333.0 31 12597.0 32 16625.0 33 21693.0 34 29737.0 35 41501.0 36 59873.0 37 94176.0 38 125938.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.585142401911966 19.222067317267477 13.076279625572596 25.116510655247957 2 13.74200120976728 20.5093359014358 43.03687402502308 22.711788863773837 3 18.467425174006998 25.02735959058114 30.598369169422522 25.906846065989342 4 11.492684828152623 17.926894019993675 39.487212121091595 31.093209030762104 5 12.318214070633411 38.24674589025168 36.978691518942306 12.456348520172599 6 30.056108870687236 37.93917663263298 19.244567496606237 12.760147000073541 7 26.618670621158124 32.48921683529065 22.9374456855382 17.954666858013027 8 23.57305162447678 38.53836954355192 21.51365357783536 16.37492525413594 9 25.722553692168876 16.241607803699125 20.79952936125896 37.23630914287304 10 14.074377405298813 30.102612830380714 35.32330396615884 20.499705798161635 11 33.266355827440506 23.440735838331804 25.045811024060228 18.247097310167465 12 22.46780787477318 25.68234983136275 32.53138719787897 19.3184550959851 13 28.14554531795308 22.98412420799911 26.11019246515991 22.7601380088879 14 19.983583522308322 23.405604259539345 28.30769169616399 28.30312052198834 15 22.656302791568454 31.584909710269937 23.849367395529832 21.909420102631778 16 20.96827728211376 29.156041745753182 28.88454727402458 20.991133698108484 17 20.900212263588447 29.32735497308943 28.410726068671643 21.361706694650483 18 20.4897816413369 28.875930861370968 30.23175392467252 20.402533572619607 19 22.911051748674282 27.71285011249712 27.868875267329724 21.507222871498875 20 22.48445797691354 28.60606735141667 28.69768976372945 20.211784907940345 21 22.367327956625953 27.745956538502814 27.608623931250225 22.27809157362101 22 21.953182761334634 28.935025082083204 28.17083641393785 20.940955742644313 23 21.670519070891526 28.48500146079988 28.16402298325148 21.68045648505711 24 22.54038981713534 27.961737740952664 28.545052359194607 20.95282008271739 25 21.973570978557476 28.039049286607217 28.60884929018461 21.378530444650696 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 240.0 1 240.0 2 315.5 3 391.0 4 391.0 5 391.0 6 505.0 7 619.0 8 619.0 9 619.0 10 913.5 11 1208.0 12 1208.0 13 1208.0 14 1996.0 15 2784.0 16 2784.0 17 2784.0 18 4884.0 19 6984.0 20 6984.0 21 6984.0 22 10841.5 23 14699.0 24 14699.0 25 14699.0 26 20921.0 27 27143.0 28 27143.0 29 27143.0 30 35210.5 31 43278.0 32 43278.0 33 43278.0 34 48747.5 35 54217.0 36 54217.0 37 54217.0 38 57536.5 39 60856.0 40 60856.0 41 60856.0 42 62854.5 43 64853.0 44 64853.0 45 64853.0 46 65489.0 47 66125.0 48 66125.0 49 66125.0 50 62123.5 51 58122.0 52 58122.0 53 58122.0 54 50308.0 55 42494.0 56 42494.0 57 42494.0 58 35490.5 59 28487.0 60 28487.0 61 28487.0 62 22627.0 63 16767.0 64 16767.0 65 16767.0 66 12617.5 67 8468.0 68 8468.0 69 8468.0 70 5993.5 71 3519.0 72 3519.0 73 3519.0 74 2429.0 75 1339.0 76 1339.0 77 1339.0 78 900.5 79 462.0 80 462.0 81 462.0 82 299.0 83 136.0 84 136.0 85 136.0 86 87.5 87 39.0 88 39.0 89 39.0 90 25.5 91 12.0 92 12.0 93 12.0 94 7.0 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22752337330723604 2 0.13293723732973006 3 0.13492434102673648 4 0.07670220270444814 5 0.022255561406471995 6 0.02305040288527457 7 0.30541783822988805 8 0.3070075211874932 9 0.01967232660036364 10 0.037556259873421496 11 0.018082643642758498 12 0.018678774751860427 13 0.017685222903357212 14 0.01828135401245914 15 0.017287802163955927 16 0.020665878448866856 17 0.019274905860962352 18 0.016294250315452712 19 0.02404395473377778 20 0.018480064382159784 21 0.017287802163955927 22 0.019274905860962352 23 0.019076195491261713 24 0.01708909179425528 25 0.01708909179425528 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 503245.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.7923746671415 #Duplication Level Percentage of deduplicated Percentage of total 1 94.83907686566641 83.26147769276419 2 3.876715304613085 6.806920850008671 3 0.6063962492953353 1.5971090014465623 4 0.22760520528965875 0.7992800583592551 5 0.11638794286078044 0.5108986943185747 6 0.06931057884719996 0.36509641839298995 7 0.047243252387051925 0.2903318119840873 8 0.02597404179729772 0.1824258247270684 9 0.022505589729043023 0.17782372490374052 >10 0.14385430288739973 2.4436057428382427 >50 0.01133212522657588 0.6932282352559592 >100 0.01223868726016355 2.0699338761068606 >500 0.0013598541400158225 0.8018680688938639 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 847 0.16830768313644445 No Hit GTCCTACAGTGGACATTTCTAAATT 799 0.15876958539081362 No Hit CTGTAGGACGTGGAATATGGCAAGA 677 0.13452692028733518 No Hit GTCCTAAAGTGTGTATTTCTCATTT 621 0.12339913958409919 No Hit CTTTAGGACGTGAAATATGGCGAGG 567 0.11266877962026448 No Hit GGTATCAACGCAGAGTACTTTTTTT 519 0.10313068187463362 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 1.9871036970064282E-4 0.0 0.0 11 0.0 0.0 1.9871036970064282E-4 0.0 0.0 12 0.0 0.0 1.9871036970064282E-4 0.0 0.0 13 0.0 0.0 1.9871036970064282E-4 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACAGTG 105 1.9363142E-8 12.687479 7 GTCCTAC 500 0.0 12.597041 1 GTATTGG 70 1.0429088E-4 12.269846 1 GTGTAGA 95 9.79866E-7 12.0545845 1 TCCTACA 535 0.0 11.763522 2 TATTGTA 65 7.759511E-4 11.736101 2 TATGAAC 75 1.9978645E-4 11.4426985 3 TAGGACC 125 1.7673301E-8 11.422149 4 GTGTAGG 85 5.066166E-5 11.227309 1 CCCTTAC 60 0.0056713293 11.133749 1 TACTATA 60 0.005705218 11.124846 2 GGTATCA 335 0.0 11.110009 1 TTAACCC 60 0.0057821013 11.104867 4 TGTAGAA 250 0.0 11.0612755 2 CCTACAG 520 0.0 11.002595 3 AATGTCC 105 3.411571E-6 10.867394 8 CATTTCT 610 0.0 10.72718 14 TTAATCC 80 3.694214E-4 10.708265 4 TCCAACG 80 3.822214E-4 10.668881 18 CCAGCAC 110 5.7550133E-6 10.402453 3 >>END_MODULE